BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0144.0001.p (350 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 437 e-124 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 164 8e-42 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 135 5e-33 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 100 1e-22 gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 95 8e-21 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 437 bits (1123), Expect = e-124, Method: Compositional matrix adjust. Identities = 206/241 (85%), Positives = 225/241 (93%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDVESNQEDRIFTADELIAKVQGKAGLFSTPSEPVSAA 60 MKPKIFVARAVFPEVL RL QHF+VESNQ+DRI++A EL AK QGK GLF+TPSEPV+AA Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVTAA 60 Query: 61 LFAANPQLKAVCNMAVGYNNIDVAAATKAGVQATNTPDVLNETTADFGWALLMATARRVT 120 LFAANPQLKAVCNMAVGYNNIDVAAAT+AGV ATNTPDVLNETTADFGWAL+MA ARR+T Sbjct: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRIT 120 Query: 121 ESEHWLRAGHWKKWSYDSFTGPDVHGSTLGIIGMGRIGQAIARRSLGFDMNVLYHNRSQL 180 ESEH+LRAG WKKWSYDSF GPD+HG+TLGIIGMGRIGQAIARRSLGFDM VLYHNRS+L Sbjct: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 Query: 181 APELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINLARG 240 APELEARAN+A+YV KEELLR ADHVILVLPYSK++HHTI AA+LALMKP ATL N+ARG Sbjct: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 Query: 241 G 241 G Sbjct: 241 G 241 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 164 bits (416), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 100/244 (40%), Positives = 145/244 (59%), Gaps = 10/244 (4%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDVESNQEDRIFTAD--ELIAKVQGKAGLFSTPSEPVS 58 MKP + V + + +++RL FD+ DRI + + IA + G+ S P++ Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDL--TLFDRITDDNRADFIAALATAHGMIGA-SLPIT 57 Query: 59 AALFAANPQLKAVCNMAVGYNNIDVAAATKAGVQATNTPDVLNETTADFGWALLMATARR 118 A+ A PQLK V ++VG +N D+ + G+ +TP VL E TAD +AL++A+ARR Sbjct: 58 NAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARR 117 Query: 119 VTESEHWLRAGHWKKWSYDSFTGPDVHGSTLGIIGMGRIGQAIARRS-LGFDMNVLYHNR 177 V E +++AG WK ++ G +VHG TLG+IGMGRIG A+ARR+ GF M +LYHNR Sbjct: 118 VVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNR 177 Query: 178 SQLAPELEA-RANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLIN 236 P+ EA R A+YV KE+LL AD V ++LP + ++ A + ALMK A IN Sbjct: 178 R---PDPEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFIN 234 Query: 237 LARG 240 +RG Sbjct: 235 ASRG 238 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 135 bits (340), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 84/240 (35%), Positives = 126/240 (52%), Gaps = 11/240 (4%) Query: 2 KPKIFVARAVFPEVLERLAQHFDVESNQEDRIFTADELIAKVQGKAGLFSTPSEPVSAAL 61 +P + + + +L+ L QH+ N + + EL A G+ + VS Sbjct: 6 RPHVLIVARLPQHLLDLLQQHYTCH-NLILQPHSEAELAAIAPQIRGIAANGEAKVSREF 64 Query: 62 FAANPQLKAVCNMAVGYNNIDVAAATKAGVQATNTPDVLNETTADFGWALLMATARRVTE 121 A P L+ V VGY+ +DV AA + G+ T+TPDVLN+ AD AL++ATAR V Sbjct: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVVR 124 Query: 122 SEHWLRAGHWKKWSYDSFTGPDVHGSTLGIIGMGRIGQAIARRSLGFDMNVLYHNRSQLA 181 ++ + R+G WKK + T V G+ LGI+G+GRIGQAIA+R+ FDM + YHNRS+ Sbjct: 125 ADRFARSGEWKKGPFPFTT--KVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR-- 180 Query: 182 PELEARANNAKYVGK-EELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINLARG 240 + YV L R D ++++ P + + A L + P+ LIN+ARG Sbjct: 181 -----KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 71/243 (29%), Positives = 114/243 (46%), Gaps = 15/243 (6%) Query: 1 MKPKIFVARAVFPEVLERLAQHFDV--ESNQEDRIFTADELIAKVQGK-AGLFSTPSEPV 57 MKP + V + E +A H+D+ +++ R DE+IA K A + + S + Sbjct: 1 MKPPLLVLIHLSEESTATIAAHYDILYAPDRQRR----DEMIAGPARKVAVVLTNGSTGL 56 Query: 58 SAALFAANPQLKAVCNMAVGYNNIDVAAATKAGVQATNTPDVLNETTADFGWALLMATAR 117 +A A P L+ VC + G+ N+DVA A G++ + AD LL+A R Sbjct: 57 TAEEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILR 116 Query: 118 RVTESEHWLRAGHWKKWSYDSFTGPDVHGSTLGIIGMGRIGQAIARRSLGFDMNVLYHNR 177 + + + R G W++ P + G +GI+GMG IG+ IARR+ FD + Y NR Sbjct: 117 NIPVLDRYTRDGGWRE---TIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNR 173 Query: 178 SQLAPELEARANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINL 237 + + + +L AD +I+ P ++ H I A L + PQ L+N+ Sbjct: 174 KK-----RDDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNI 228 Query: 238 ARG 240 RG Sbjct: 229 GRG 231 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 95.1 bits (235), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 56/174 (32%), Positives = 90/174 (51%), Gaps = 6/174 (3%) Query: 68 LKAVCNMAVGYNNIDVAAATKAGVQATNTPDVLNETTADFGWALLMATARRVTESEHWLR 127 ++ V + G+N+IDVAAA G+ D + A+F LL A R++ + R Sbjct: 69 VRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKIARASMRTR 128 Query: 128 AGHWKKWSYDSFTGPDVHGSTLGIIGMGRIGQAIARRSLGFDMNVLYHNRSQLAPELEAR 187 G+++ D G D+HG T+G+IG G+IG AR GF +L + + P+ +A Sbjct: 129 EGNFE---LDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHR-NPDFDAL 184 Query: 188 ANNAKYVGKEELLRTADHVILVLPYSKESHHTIKAADLALMKPQATLINLARGG 241 +YV + EL +D + L P ++ + + + A LAL KP L+N +RGG Sbjct: 185 GG--RYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGG 236 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 923,582 Number of extensions: 34122 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 9 Length of query: 350 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 264 Effective length of database: 1,212,660 Effective search space: 320142240 Effective search space used: 320142240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)