BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0143.0017.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 698 0.0 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 354 7e-99 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 249 3e-67 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 184 1e-47 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 129 3e-31 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/380 (91%), Positives = 361/380 (95%) Query: 1 MKLKSTIIPLTGAIALAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAIDELNA 60 MKLKS IIPLT AI LAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMA+DELNA Sbjct: 1 MKLKSAIIPLTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELNA 60 Query: 61 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDA 120 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIY+DA Sbjct: 61 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA 120 Query: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQ 180 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGA LGKYAV L TK +AVIDDRTAYGQ Sbjct: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ 180 Query: 181 GVAEEFAKGAQGAGATIVGKQFTNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQ 240 GVA+EFAKGA+ AG TIVG Q+TNDKATDFNAILTS+K KKP+VVFFGGMDAVGGPMLRQ Sbjct: 181 GVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQ 240 Query: 241 MKQLGINAKFMGGDGICTGSLPGLAGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKK 300 MKQLGI+AKFMGGDGICTGSLPGLAGDGL DDQV+CAEAGGVDE+GKKGMDDFRAAYKKK Sbjct: 241 MKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKK 300 Query: 301 FGIDVVYNAAYAYDATMTVADAMKKANSAEPAKYLPELAKISHKGVTGTIAFDAKGDIKN 360 FGIDVVYNAAYAYDATMTVADAM KA SA+P KYLPELAKISHKGVTG IAFDAKGDIK+ Sbjct: 301 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKD 360 Query: 361 GSLTLYTYKKGERTLLAVVK 380 GSLTLYTYK G+RTLLAV K Sbjct: 361 GSLTLYTYKGGQRTLLAVTK 380 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 354 bits (908), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 193/365 (52%), Positives = 242/365 (66%), Gaps = 4/365 (1%) Query: 7 IIPLTGAIALAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFTIG 66 II + A+ AFA A +Q VVKI PL+GP A GKDN+ G MAI+ LNA+ T+G Sbjct: 10 IIAASLALIPAFAMAQETQ-VVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITVG 68 Query: 67 GKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQVS 126 GKK+KF+++ EDD +DPK AVA KL DQ V A++G NSG TIPAS++YNDAGI V+ Sbjct: 69 GKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI-VVA 127 Query: 127 PSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEEF 186 A+NPK TQQGF T FRV A+D+QLG + YA LK K VAVIDDRTAYGQG+A+EF Sbjct: 128 TVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQEF 187 Query: 187 AKGAQGAGATIVGKQFTNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLGI 246 K A+ G +V FTNDKATDF AILTSIK KKP+ VF GG GGP+ RQMKQLG+ Sbjct: 188 IKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLGV 247 Query: 247 NAKFMGGDGICTGSLPGLAGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDVV 306 + MGGDGIC+ + L GD I + V C + G + + K+G F Y+KK+ Sbjct: 248 DVPLMGGDGICSPEMGRLGGDA-IGESVYCTQGGTMLDKAKEG-KVFSDEYQKKYNRPAE 305 Query: 307 YNAAYAYDATMTVADAMKKANSAEPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTLY 366 A YD M +A AMK+ANS +P ++ P LAKIS+KGV G FDA D+K +T+Y Sbjct: 306 TYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSPVTVY 365 Query: 367 TYKKG 371 +K G Sbjct: 366 RFKDG 370 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 249 bits (635), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 125/342 (36%), Positives = 200/342 (58%), Gaps = 3/342 (0%) Query: 28 VKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFTIGGKKVKFELVGEDDASDPKQAT 87 + I GPLSGP+ +GK +NG ++A+ E N +G IGGK + L+ +DD +DP A Sbjct: 40 LAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRADPATAE 99 Query: 88 AVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQVSPSATNPKYTQQGFKTAFRVVA 147 VA LV Q+V AV+GH SGT++ A+ +Y+ AG+ QV+PSA + + T + FR + Sbjct: 100 FVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRIFRTIP 159 Query: 148 NDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEEFAKGAQGAGATIVGKQFTNDKA 207 N+ LG + YAV L+T+ + IDDRT +GQG+AE+FA+ A GA +VG+ +DK Sbjct: 160 NNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYSVSDKT 219 Query: 208 TDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLGINAKFMGGDGICTGSLPGLAGD 267 +DFNA L + +P+++FFGG+D G + + +++L ++A+ M G T LP L Sbjct: 220 SDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPG--TLGLPFLMRA 277 Query: 268 GLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMKKAN 327 G + + E G + G FR Y + F D+ A +AY+A + +++A Sbjct: 278 GPDANGALVLEPGPPQDK-MPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAG 336 Query: 328 SAEPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTLYTYK 369 SA+P + + ++ +GV+G +AF+ +GD+ S T+Y K Sbjct: 337 SADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVK 378 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 124/349 (35%), Positives = 184/349 (52%), Gaps = 14/349 (4%) Query: 26 EVVKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFTIGGKKVKFELVGEDDASDPKQ 85 E VKIA GP++G A G + GA AI+++NA G G KFE V DDA +PKQ Sbjct: 27 ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACEPKQ 83 Query: 86 ATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQVSPSATNPKYTQ-QGFKTAFR 144 A AVA K+V Q + VIGH+ SG+TIPAS IY + G+ ++PSAT P+ T+ + K FR Sbjct: 84 AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFR 143 Query: 145 VVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEEFAKGAQGAGATIVGKQFTN 204 + D Q G +Y + LK K VAV+ D+ +YGQGVA A +V + N Sbjct: 144 TIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 205 DKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLGINAKFMGGDGICTGSLPGL 264 +D++AI+T +K++ + V+FGG G ++RQ ++ GI A FMG +G+ + + Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAI 263 Query: 265 AGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDVVYNAAY---AYDATMTVAD 321 AG + +V A + + AA K+ N + AY +AD Sbjct: 264 AGPAS-EGMLVTLPADFAADPANAALVKAFAAAKRD------PNGPFQMPAYSGVKIIAD 316 Query: 322 AMKKANSAEPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTLYTYKK 370 A+ A S +P K + S K G + +D KGD+K+ ++T+ K Sbjct: 317 AIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTWHK 365 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 103/373 (27%), Positives = 178/373 (47%), Gaps = 30/373 (8%) Query: 10 LTGAIAL-AFAGAAYSQE--VVKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFTIG 66 L G + L AF A ++QE + + G LS P + + GA++AIDE NA + Sbjct: 10 LAGVLLLWAFMQAGWAQERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANA----LA 62 Query: 67 GKK---VKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIP 123 K+ ++FEL+ +DD + A +A V +KVA VIG +S + + IY A IP Sbjct: 63 AKRRSPLRFELLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIP 122 Query: 124 QVSPSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVA 183 Q+ +AT+ ++T G + FRVV +L A L + A +L+ + + VI AY + +A Sbjct: 123 QIGFTATSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALA 182 Query: 184 EEFAKGAQGAGATIVGKQF-TNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMK 242 + + G+ I ++ ++TDF+A L + +A + +V+ F G+ + Sbjct: 183 DTLVENLGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGAN 242 Query: 243 QLGINAKFMGGDGICTGSLPGLAGDGL--IDDQVV---CAEAGGVDESGKKGMDDFRAAY 297 + A+ + G SLP L ++ ++ C + V ++ Y Sbjct: 243 RTRTTARLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWKVVTQT-----------Y 291 Query: 298 KKKFGIDVVYNAAYAYDATMTVADAMKKANSAEPAKYLPELAKISHKGVTGTIAFDAKGD 357 +++FG + YAYDA + A+ +A S K L ++ H G+ GT++FDA G Sbjct: 292 QRRFGAAPTTYSFYAYDAASVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGA 351 Query: 358 IKNGSLTLYTYKK 370 + TL Y++ Sbjct: 352 NAHPRYTLLRYQE 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,141,437 Number of extensions: 49985 Number of successful extensions: 169 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 14 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)