BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0143.0016.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 238 6e-64 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 229 5e-61 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 218 8e-58 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 194 1e-50 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 145 6e-36 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 238 bits (608), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 154/454 (33%), Positives = 234/454 (51%), Gaps = 55/454 (12%) Query: 1 MPFNRRSKNITQGVSRSPNRSMYYALGYEKEDFDKPMIGVANGHSTITPCNSGLQKLADV 60 MP RS TQG + + R+++ A G + DF KP+I V N + P + L+ L + Sbjct: 1 MP-TYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQL 59 Query: 61 AISAIKQAGANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVI 120 I+ AG + F T + D + G M YSL SR++IAD +E VN D +V I Sbjct: 60 VAREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCI 119 Query: 121 GGCDKNMPGGMIALARTNVPGIYVYGGTIKPG--------NWKGKDLTIVSAFEAVGEFS 172 CDK PG ++A R N+P ++V GG ++ G + K L +V A G+ Sbjct: 120 SNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGD-- 177 Query: 173 AGRMSQEDFDGIERNACPSSGSCGGMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSA 232 +S D +ER+ACP+ GSC GM+TAN+M+ E LG+ L + T+ ++ Sbjct: 178 -KNISDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELF 236 Query: 233 AESARVLVEAVKK-------DIKPRDIITRKSIENAVSLIMATGGSTNAVLHFLAIAHAA 285 + R++VE K+ I PR+I T+++ ENA++L ++ GGSTN VLH LA AH A Sbjct: 237 LRAGRLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEA 296 Query: 286 EVEWTIDDFERVRQKVPVICDLKP-SGKYVATDLHKAGGIPAVLKVLHEAGMLHGDCLTI 344 +VE+T+ D +R+ ++VP +C + P + KY D+H+AGGI ++L L AG+L T+ Sbjct: 297 KVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTV 356 Query: 345 TGKTLAEELENV-------PALRA------------------------DQD----VIRPI 369 KTL E +E PA+ D D IR Sbjct: 357 HSKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDR 416 Query: 370 DKALYQQGHLAILKGNLAPEGCVAKITGXEKAFV 403 + A Q G LA+L GN+A +GC+ K G +++ + Sbjct: 417 EHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESIL 450 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 229 bits (583), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 139/394 (35%), Positives = 225/394 (57%), Gaps = 14/394 (3%) Query: 20 RSMYYALGYEKEDFD-KPMIGVANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTP 78 RS G + FD +P+IG+ N +S +TPCNS + LA+ I +AG P F Sbjct: 27 RSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPV- 85 Query: 79 TISDGMSMGTEGMK-YSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALART 137 MS+G ++ +++ R + + +E ++ +DGVV++ GCDK P ++ A Sbjct: 86 -----MSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASV 140 Query: 138 NVPGIYVYGGTIKPGNWKGKDL-TIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSCG 196 +VP I V GG + G ++G++L + ++ + AG+M+QE+F E S G C Sbjct: 141 DVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCM 200 Query: 197 GMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRK 256 M TA+TM+S EALG+GL +++ + D + A + R +V+ V++D+ I+TR+ Sbjct: 201 TMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMVEEDLVLSKILTRQ 260 Query: 257 SIENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVAT 316 + ENA+ + A GGSTNAV+H LAIA ++ + D++ + Q++P + DL+PSGK++ Sbjct: 261 AFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLME 320 Query: 317 DLHKAGGIPAVLKVLHEAGMLHGDCLTITGKTLAEELENVPALRADQDVIRPIDKALYQQ 376 D + AGG+PAV++ L ++ LT GKTL E ++ P +++VIR DK + Sbjct: 321 DFYYAGGLPAVIRQLE--SVIDKTALTANGKTLWENCQDAP--NWNEEVIRSFDKPFKEA 376 Query: 377 GHLAILKGNLAPEGCVAKITGXEKAFV-HRIRCT 409 +AILKGNLAP+G V K + A + HR R Sbjct: 377 AGIAILKGNLAPDGAVIKPSAATPALLKHRGRAV 410 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 218 bits (555), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 134/380 (35%), Positives = 216/380 (56%), Gaps = 14/380 (3%) Query: 20 RSMYYALGYEKEDFD-KPMIGVANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTP 78 RS G +F KP+IG+ N S +TPCN+ +K+A+ I +AG P F P Sbjct: 29 RSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEHVRRGIIEAGGFPVEF--P 86 Query: 79 TISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALARTN 138 S+G S ++++R + + +E ++ G +D VV++ GCDK P ++ A + Sbjct: 87 VFSNGES---NLRPTAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCD 143 Query: 139 VPGIYVYGGTIKPGNWKGKDL---TIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSC 195 VP I V GG + G +G+D+ T+V ++ + AG ++ DF E S+G+C Sbjct: 144 VPAIVVTGGPMLNGKHQGRDIGSGTVV--WQLSEQVKAGEITIHDFMAAEAGMSRSAGTC 201 Query: 196 GGMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITR 255 M TA+TM+ E+LG+ L +++ + D + A S +V+ V + + I+TR Sbjct: 202 NTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLSKILTR 261 Query: 256 KSIENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVA 315 K+ ENA+ A GGSTNAV+H AIA V+ ++D+ R+ + P I DL+PSG+Y+ Sbjct: 262 KAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRYLM 321 Query: 316 TDLHKAGGIPAVLKVLHEAGML-HGDCLTITGKTLAEELENVPALRADQDVIRPIDKALY 374 + + AGG+PAVL+ L EA +L H D LT+ G+T+ + +++ P + +V+RP+ K L Sbjct: 322 EEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIY--NDEVVRPLAKPLI 379 Query: 375 QQGHLAILKGNLAPEGCVAK 394 + G + IL+GNLAP G V K Sbjct: 380 EDGGICILRGNLAPRGAVLK 399 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 194 bits (493), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 129/391 (32%), Positives = 204/391 (52%), Gaps = 9/391 (2%) Query: 20 RSMYYALGYEKEDFDKPMIGVANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTPT 79 R M Y + E+ +P+IG+A S I+PCN +LA I+ AG P F Sbjct: 32 RFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAKRVRDGIRDAGGIPMEFPLHP 91 Query: 80 ISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALARTNV 139 I + T + I R + + ++G +D VV+ GCDK P ++A A ++ Sbjct: 92 IFENCRRPT-----AAIDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDI 146 Query: 140 PGIYVYGGTIKPGNWKGKDLTIVSA-FEAVGEFSAGRMSQEDFDGIERNACPSSGSCGGM 198 P I + GG + G G+ + SA ++ SAG + E F I + PSSG C M Sbjct: 147 PAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLEIAAASAPSSGHCNTM 206 Query: 199 YTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRKSI 258 TA+TM++ EALGM L S + P E+ A E+ R +V +D++P I+TR + Sbjct: 207 GTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMAYEDLRPSAILTRDAF 266 Query: 259 ENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVATDL 318 +A+ + A GGSTNA H +A+A A VE +D+ + VP++ +++P+GKY+ Sbjct: 267 LDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQPAGKYLGERF 326 Query: 319 HKAGGIPAVLKVLHEAGMLHGDCLTITGKTLAEELENVPALRADQDVIRPIDKALYQQGH 378 H+AGG+PA++ L +AG L + +T TGKT+AE L+ + D+++I P L ++ Sbjct: 327 HRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRAS--NDREMIYPFAAPLRERAG 384 Query: 379 LAILKGNLAPEGCVAKITGXEKAFVHRIRCT 409 +LKGNL + + K + + F R T Sbjct: 385 FLVLKGNLF-DFAIMKTSVISETFRERYLST 414 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 145 bits (367), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 113/382 (29%), Positives = 183/382 (47%), Gaps = 33/382 (8%) Query: 30 KEDFDKPMIGVANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFG-TPTISDGMSMGT 88 KED P + + + ++ + + ++ + A ++AGA Q G +P + DG++ G Sbjct: 63 KED-KAPSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQ 121 Query: 89 EGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMI-ALARTNVPGIYVYGG 147 GM+ SL SR+ IA A++ D + +G CDK +PG +I AL ++P ++V G Sbjct: 122 AGMELSLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAG 181 Query: 148 TIKPGNWKGKDLTIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSCGGMYTANTMSSA 207 + G + I + +AG++ + + E + +G+C TAN+ Sbjct: 182 PMTSGMSNSDKVKIRQLY------TAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQML 235 Query: 208 FEALGMGL----FYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRKSIENAVS 263 EA+G+ L F + D +A ++A++ + R +I KSI NA+ Sbjct: 236 MEAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGR-MIDEKSIVNAIV 294 Query: 264 LIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVATDLHKAGG 323 + ATGGSTN LH +AIA AA + DDF+++ VP+I + P+G H AGG Sbjct: 295 ALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGG 354 Query: 324 IPAVLKVLHEAGMLHGDCLTITGK---------TLAEELEN----------VPALRADQD 364 VL+ L +AG++H D TI G+ LA E EN PA D+ Sbjct: 355 TGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEG 414 Query: 365 VIRPIDKALYQQGHLAILKGNL 386 V+ + K G L +L+GNL Sbjct: 415 VLGTVAKPFAADGGLKLLQGNL 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,619,062 Number of extensions: 71740 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 5 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)