BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0143.0009.p (470 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 426 e-120 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 90 4e-19 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 84 2e-17 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 76 5e-15 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 59 1e-09 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 426 bits (1095), Expect = e-120, Method: Compositional matrix adjust. Identities = 198/220 (90%), Positives = 212/220 (96%) Query: 2 QRGALSCTNLAHGFAAFPANDKLKLKQDRAPSVAIVSSYNDMLSAHQPFERYPQIIRDAV 61 QRGAL+CTNLAHGFAAFPANDKLKLK+D+APS+AIVS+YNDMLSAHQPFER+PQII+DA Sbjct: 38 QRGALACTNLAHGFAAFPANDKLKLKEDKAPSLAIVSAYNDMLSAHQPFERFPQIIKDAA 97 Query: 62 REDGGVAQFAGGVPAMCDGITQGQTGMELSLFSRDAIAMGTAVALSHNMFDAALYLGVCD 121 RE G VAQFAGG PAMCDG+TQGQ GMELSLFSRDAIAM TA+ALSHNMFDAALYLGVCD Sbjct: 98 REAGAVAQFAGGSPAMCDGVTQGQAGMELSLFSRDAIAMATAIALSHNMFDAALYLGVCD 157 Query: 122 KIVPGLLIGALHFGHIPAVFVPAGPMTSGMTNSEKVKIRQLYTEGKIGRAELLEAESKSY 181 KIVPGLLIGALHFGH+PAVFVPAGPMTSGM+NS+KVKIRQLYT GKIGRAELLEAESKSY Sbjct: 158 KIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKVKIRQLYTAGKIGRAELLEAESKSY 217 Query: 182 HDAGTCTFYGTANSNQMLMEAMGLHLPGSAFITPNTPMRD 221 H AGTCTFYGTANSNQMLMEAMGLHLPG+AFITPNTP+RD Sbjct: 218 HGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRD 257 Score = 339 bits (870), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 172/256 (67%), Positives = 194/256 (75%), Gaps = 9/256 (3%) Query: 221 DALTAAAAKXRAHATEPLFDEAGK------VTWKATPKDSGDDTVLRPAANPFSADGGLK 274 D T R H EP+ G+ VTW P SGD+ VL A PF+ADGGLK Sbjct: 371 DVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGTVAKPFAADGGLK 430 Query: 275 LLQGNLGRSVIKVSAVKHEHRAVQAPAIVFHSQEAFMKAFKAGELDKDFVAVLTFQGPRA 334 LLQGNLGR+VIK+SAVK EHR V+APA+VFHSQEAFM AFKAG+LD+DFVAV+TFQGPRA Sbjct: 431 LLQGNLGRAVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQGPRA 490 Query: 335 NGMPELHALTPALGILQDKGSGASGKVPAARXRMSGASGKVPAAIHVTPEVLAGGPLGFV 394 NGMPELHALTPALGILQDKG + RMSGASGKVPAAIHVTPEVL GGPLG V Sbjct: 491 NGMPELHALTPALGILQDKGRHVA---LVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRV 547 Query: 395 RTGDMIRLDAEAGILEALVSEAEWASRTSETADLSGNQTGMGRELFSVFRASVSAAEEGG 454 R GD+IRLDAEAG LEA V EWA+R+ +++DLSGNQ GMGRELFS+FR +V AEEGG Sbjct: 548 RDGDIIRLDAEAGTLEAKVDATEWAARSQDSSDLSGNQHGMGRELFSLFRNAVGTAEEGG 607 Query: 455 TSFGLPPVLENEKATV 470 +FGLPP+ E EK TV Sbjct: 608 ATFGLPPIFEKEKVTV 623 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 90.1 bits (222), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 64/203 (31%), Positives = 97/203 (47%), Gaps = 13/203 (6%) Query: 32 PSVAIVSSYNDMLSAHQPFERYPQIIRDAVREDGGVAQFAGGVPAMCDGITQGQTGMELS 91 P +A+V+S+ + H + Q++ + GGVA+ + A+ D T G M S Sbjct: 35 PIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTI-AVDDSGTHGDARMLYS 93 Query: 92 LFSRDAIAMGTAVALSHNMFDAALYLGVCDKIVPGLLIGALHFGHIPAVFVPAGPMTSG- 150 L SRD IA ++ + DA + + CDKI PG+L+ A+ +IP VFV GPM +G Sbjct: 94 LPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRL-NIPVVFVSGGPMEAGK 152 Query: 151 ---MTNSEKVKIRQLYTEG-------KIGRAELLEAESKSYHDAGTCTFYGTANSNQMLM 200 N++K I+ + I A++ E E + G+C+ TANS L Sbjct: 153 VIKTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCLT 212 Query: 201 EAMGLHLPGSAFITPNTPMRDAL 223 E +GL LPG+ I R L Sbjct: 213 EVLGLSLPGNGTIVATHADRKEL 235 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 50/203 (24%), Positives = 90/203 (44%), Gaps = 12/203 (5%) Query: 259 VLRPAANPFSADGGLKLLQGNLGR--SVIKVSAVKHEHRAVQAPAIVFHSQEAFMKAFKA 316 +R + +S DGGL +L GN+ ++K + V A VF SQ+A ++ Sbjct: 412 CIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILG 471 Query: 317 GELDKDFVAVLTFQGPRANGMPELHALTPALGILQDKGSGASGKVPAARXRMSGASGKVP 376 ++ V ++ ++GP+ G P + + ++ KG G + + G+SG V Sbjct: 472 DKVKAGDVVIVRYEGPK--GGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVI 529 Query: 377 AAIHVTPEVLAGGPLGFVRTGDMIRLDAEAGILEALVSEAEWASRTS------ETADLSG 430 H +PE GG +G V GDMI +D + +S + A R + + A G Sbjct: 530 G--HASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPG 587 Query: 431 NQTGMGRELFSVFRASVSAAEEG 453 N+ + + + A ++A+ G Sbjct: 588 NRQRVVSQALQAYAALTTSADRG 610 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 98/305 (32%), Positives = 134/305 (43%), Gaps = 44/305 (14%) Query: 166 GKIGRAELLEAESKSYHDAGTCTFYGTANSNQMLMEAMG--------LHLPGSAFITPNT 217 G+IG LE E + GT T S + LME L G A + P+ Sbjct: 289 GRIGVD--LELEDWTRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPH- 345 Query: 218 PMRDALTAAAAKXRAHATEPLFDEAGKVTWKATPKDS--GDDTVLRPAANPFSADGGLKL 275 +DALT G+ W KD+ +D V+RP A P DGG+ + Sbjct: 346 --KDALTVN----------------GQTMWDNV-KDAPIYNDEVVRPLAKPLIEDGGICI 386 Query: 276 LQGNLGR--SVIKVSAVKHEHRAVQAPAIVFHSQEAFMKAFKAGELDKDFVAVLTFQ--G 331 L+GNL +V+K SA E + A+VF + + +LD D VL + G Sbjct: 387 LRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVG 446 Query: 332 PRAN-GMPELHALTPALGILQDKGSGASGKVPAARXRMSG-ASGKVPAAIHVTPEVLAGG 389 P+ GM E+ + +L +G V + RMSG A G V +HV PE AGG Sbjct: 447 PKGYPGMAEVGNMGLPPKVL---ATGVKDMVRISDARMSGTAYGTV--ILHVAPEAAAGG 501 Query: 390 PLGFVRTGDMIRLDAEAGILEALVSEAEWASRTSETAD-LSGNQTGMGRELFSVFRASVS 448 PLG V+ GD I LDA AG L+ +SE E R A L+ + M S++ V Sbjct: 502 PLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVLAERKPEMVGGYQSLYVDRVL 561 Query: 449 AAEEG 453 A+EG Sbjct: 562 QADEG 566 Score = 72.8 bits (177), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 48/190 (25%), Positives = 91/190 (47%), Gaps = 16/190 (8%) Query: 32 PSVAIVSSYNDMLSAHQPFERYPQIIRDAVREDGGVAQFAGGVPAMCDGITQGQTGME-L 90 P + I ++++++ + F + + +R + E AGG P + G++ + Sbjct: 46 PVIGICNTWSELTPCNAHFRKIAEHVRRGIIE-------AGGFPVEFPVFSNGESNLRPT 98 Query: 91 SLFSRDAIAMGTAVALSHNMFDAALYLGVCDKIVPGLLIGALHFGHIPAVFVPAGPMTSG 150 ++ +R+ +M ++ N DA + L CDK P LL+GA +PA+ V GPM +G Sbjct: 99 AMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASC-DVPAIVVTGGPMLNG 157 Query: 151 MTNSEKV-------KIRQLYTEGKIGRAELLEAESKSYHDAGTCTFYGTANSNQMLMEAM 203 + ++ + G+I + + AE+ AGTC GTA++ + E++ Sbjct: 158 KHQGRDIGSGTVVWQLSEQVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESL 217 Query: 204 GLHLPGSAFI 213 G+ LP +A I Sbjct: 218 GVSLPHNAAI 227 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 69/194 (35%), Positives = 93/194 (47%), Gaps = 22/194 (11%) Query: 243 GKVTWK-ATPKDSGDDTVLRPAANPFSADGGLKLLQGNLGR--SVIKVSA-----VKHEH 294 GK W+ + ++ V+R PF G+ +L+GNL +VIK SA +KH Sbjct: 348 GKTLWENCQDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRG 407 Query: 295 RAVQAPAIVFHSQEAFMKAFKAGELDKDFVAVLTFQ--GPRAN-GMPELHALTPALGILQ 351 RAV VF + + K LD D VL + GP+ GM E + IL+ Sbjct: 408 RAV-----VFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILR 462 Query: 352 DKGSGASGKVPAARXRMSG-ASGKVPAAIHVTPEVLAGGPLGFVRTGDMIRLDAEAGILE 410 G + V + RMSG A G V +HV+PE AGGPL V+ GD I LD EA L Sbjct: 463 ---KGITDMVRVSDARMSGTAYGTV--VLHVSPEAAAGGPLAVVQNGDFIELDVEARKLH 517 Query: 411 ALVSEAEWASRTSE 424 VS+ E A R ++ Sbjct: 518 LDVSDEELARRRAQ 531 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 55/216 (25%), Positives = 97/216 (44%), Gaps = 16/216 (7%) Query: 29 DRAPSVAIVSSYNDMLSAHQPFERYPQIIRDAVREDGGVAQFAGGVPAMCDGITQGQTGM 88 D P + I ++Y+++ + F + ++ + E AGG P ++ G+T + Sbjct: 41 DGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWE-------AGGFPLEFPVMSLGETLL 93 Query: 89 E-LSLFSRDAIAMGTAVALSHNMFDAALYLGVCDKIVPGLLIGALHFGHIPAVFVPAGPM 147 ++ R+ +M ++ N D + L CDK P LL+GA +P + V GPM Sbjct: 94 RPTAMLYRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASV-DVPTIGVSGGPM 152 Query: 148 TSGMTNSEKV-------KIRQLYTEGKIGRAELLEAESKSYHDAGTCTFYGTANSNQMLM 200 SG ++ ++ + GK+ + E EAES + G C GTA++ ++ Sbjct: 153 LSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCMTMGTASTMASMV 212 Query: 201 EAMGLHLPGSAFITPNTPMRDALTAAAAKXRAHATE 236 EA+G+ LP +A I R+ L A + E Sbjct: 213 EALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMVE 248 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 51/188 (27%), Positives = 79/188 (42%), Gaps = 19/188 (10%) Query: 252 KDSGDDTVLRPAANPFSADGGLKLLQGNL-GRSVIKVSAVKHEHRA-----------VQA 299 + S D ++ P A P G +L+GNL +++K S + R + Sbjct: 364 RASNDREMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENIFEC 423 Query: 300 PAIVFHSQEAFMKAFKAGELDKDFVAVLTFQGPRANGMP---ELHALTPALGILQDKGSG 356 A+VF + + L D +L +G G P E+ + P +++ G Sbjct: 424 RAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIK---RG 480 Query: 357 ASGKVPAARXRMSGASGKVPAAIHVTPEVLAGGPLGFVRTGDMIRLDAEAGILEALVSEA 416 S R SG S P+ ++ +PE GG L ++R GD +R+D G LVSE Sbjct: 481 VSTLPTLGDGRQSGTSDS-PSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEE 539 Query: 417 EWASRTSE 424 E A R SE Sbjct: 540 ELARRKSE 547 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 50/191 (26%), Positives = 80/191 (41%), Gaps = 18/191 (9%) Query: 32 PSVAIVSSYNDMLSAHQPFERYPQIIRDAVREDGGVAQFAGGVPAM--CDGITQGQTGME 89 P + I S +D+ ++ + +RD +R+ GG+ P C T Sbjct: 48 PIIGIAQSGSDISPCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPT------- 100 Query: 90 LSLFSRDAIAMGTAVALSHNMFDAALYLGVCDKIVPGLLIGALHFGHIPAVFVPAGPMTS 149 + R+ +G L DA + CDK P ++ A IPA+ + GPM Sbjct: 101 -AAIDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATV-DIPAIVLSGGPMLD 158 Query: 150 GMTNSEKV-------KIRQLYTEGKIGRAELLEAESKSYHDAGTCTFYGTANSNQMLMEA 202 G + E V K R+L + G I + LE + S +G C GTA++ + EA Sbjct: 159 GWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEA 218 Query: 203 MGLHLPGSAFI 213 +G+ L G + I Sbjct: 219 LGMSLTGCSAI 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,557,673 Number of extensions: 69178 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 216 Number of HSP's successfully gapped: 10 Length of query: 470 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 381 Effective length of database: 1,198,455 Effective search space: 456611355 Effective search space used: 456611355 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)