BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0141.0018.p (570 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 661 0.0 gi|300311810|ref|YP_003775902.1| two component sensor histidine ... 139 6e-34 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 82 1e-16 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 79 1e-15 gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 79 1e-15 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 661 bits (1706), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/552 (59%), Positives = 410/552 (74%), Gaps = 7/552 (1%) Query: 22 FEARAIWVQHTNTVLQRLEETHSTLRNLVANARGYALSGRQEYLLGFEADRSLLYELLNG 81 FE RA+W+QHT+TV+ LEET+S LR V N+RGY LSG +EY FEA + L+ Sbjct: 62 FEQRAVWLQHTHTVINVLEETNSALRETVGNSRGYVLSGNEEYRTSFEAGVRHVQALMQD 121 Query: 82 LRALQLDNQEQLDRLTHLSSSIAQRIELARTLIEADRRVRIDNAYE---HGRDLSEDIRK 138 LR L DN+ Q++RL + QR+ L+R ++ +R + A E G+ LS++IR+ Sbjct: 122 LRRLMSDNERQIERLDSTEDLVNQRLALSREILA----LRPEQARERVLEGKALSDEIRR 177 Query: 139 QFVALKQVEQTLLSERSADTRESIRWTLAAILGGSFVSVVILIAVYLRLKRETMRRREAQ 198 +F +K E LL++RS DT S T+ ++ GS +S+ +L+ VY +L+RE RR +AQ Sbjct: 178 RFAEMKGNENALLAKRSQDTSASADLTIFMVVSGSLLSMALLVLVYSKLRREMDRRIDAQ 237 Query: 199 TKAQLYAVEIESLYNQAPCGYHSIDEESGLIVQINDTELAWLGYAREQVVGKMTQADLMT 258 +AQ Y+ EIE LYN APCGYHS+DE I++INDTEL WLGY REQVVG+MT DL+ Sbjct: 238 QRAQAYSDEIEDLYNNAPCGYHSVDESDKKIIKINDTELKWLGYTREQVVGRMTHTDLLA 297 Query: 259 PSSAERYRKELRPRFLRNEAVGGVDVDYLRADGTHFSTLMSISTVPHPDDGHVISRNVIY 318 P+SAERY +EL+P+F+ + G+D++Y RADG+ F+ L++ + +P D +ISR VIY Sbjct: 298 PASAERYLRELQPQFMLKREISGIDLNYRRADGSEFTALVNATAIPSRDRSKLISRTVIY 357 Query: 319 DISGRKQTEKEIEELNASLKRQTRHLSNINKELESFSYSVSHDLRAPLRAISGYAMMVEE 378 DIS RK+ E+EIE LNA LKRQ HL ++NKELESFSYSVSHDLRAPLRAISGYAM++EE Sbjct: 358 DISDRKRAEEEIEALNADLKRQALHLHSVNKELESFSYSVSHDLRAPLRAISGYAMILEE 417 Query: 379 DYAKELDAKGQEMLRVIRRNVGKMDALISDLLKLSKSATDELTPGHFSMQDQVEQCIAGL 438 DYA +D KG+E L+VIRRNV KMD LI+DLLKL+KS T ELT FSM D V Q IAGL Sbjct: 418 DYADAIDEKGREQLQVIRRNVRKMDELINDLLKLAKSTTGELTLERFSMDDLVRQVIAGL 477 Query: 439 RRENDSVKFVVDPLEDVIASKDLLGQVWENLLSNAVKFSKKAERAVVRVSSTSTPEEVIY 498 RREN +V F V PLE +A++ L+ QVWENLLSNAVKFS K+E+ VRVS PEE IY Sbjct: 478 RRENPNVLFDVPPLEGAVANRGLITQVWENLLSNAVKFSSKSEQPAVRVSMQVEPEEYIY 537 Query: 499 QVQDNGIGFDMRYAHKLFGTFQRLHRQEDYAGTGIGLALVQRIVVRHGGRVWAEGSPGEG 558 V DNG+GFDMRYAHKLFGTFQRLHRQE+YAGTGIGLALVQRIV+RH GRVWA+ PGEG Sbjct: 538 GVHDNGVGFDMRYAHKLFGTFQRLHRQEEYAGTGIGLALVQRIVIRHTGRVWAQSKPGEG 597 Query: 559 ASFYFALPRQGL 570 ASFYF+LPR+GL Sbjct: 598 ASFYFSLPRKGL 609 >gi|300311810|ref|YP_003775902.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 535 Score = 139 bits (350), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 89/280 (31%), Positives = 136/280 (48%), Gaps = 38/280 (13%) Query: 324 KQTEKEIEELNASLKRQTRHLSNINKEL-------ESFSYSVSHDLRAPLRAISGYAMMV 376 ++ ++ E+ + L+R+ R L+ +N +L E F YSVSHDLR+PL + G++ + Sbjct: 250 QEASQQSEQYRSQLERRARELTELNADLLQQTQDNEMFIYSVSHDLRSPLVNLQGFSKEI 309 Query: 377 EEDYAKELDAKG--------------------QEMLRVIRRNVGKMDALISDLLKLSKSA 416 L+A Q LR I+ V + +I +L+LS++ Sbjct: 310 GHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIIDSMLRLSRAG 369 Query: 417 TDELTPGHFSMQDQVEQCIAGLR---RENDSVKFVVDPLEDVIASKDLLGQVWENLLSNA 473 E P M ++ +A + R+ +V V L + QV NL+ NA Sbjct: 370 RVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAVVEVAPELPPAFGDPTAIEQVLGNLVGNA 429 Query: 474 VKFSKKAERAVVRV-------SSTSTPEEVIYQVQDNGIGFDMRYAHKLFGTFQRLHRQE 526 V + A + + + + PE +Y V+DNG+G Y HK+F FQRLH Sbjct: 430 VNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFIAFQRLHGNA 489 Query: 527 DYAGTGIGLALVQRIVVRHGGRVWAEGSPGEGASFYFALP 566 G GIGLALV+R+V RHGGR+W E + G+G+SFY ALP Sbjct: 490 -APGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALP 528 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 82.0 bits (201), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 70/242 (28%), Positives = 113/242 (46%), Gaps = 26/242 (10%) Query: 342 RHLSNINKELESFSYSVSHDLRAPLRAISGYAMMVEEDYA-KELDAKGQEMLRVIRRNVG 400 R ++ + + F ++SH+LR PL AI G+ + D++ + KG L+VI RN Sbjct: 41 RQADSVTRLKDEFLATLSHELRTPLNAIVGWTQYLLNDHSDADRLVKG---LQVIERNAR 97 Query: 401 KMDALISDLLKLSKSATDE--LTPGHFSMQDQVEQCIAGLRRENDSVKFVVDPLED---- 454 ++ DLL +S+ + L + E+ IA +R ++ + + P Sbjct: 98 LQAQMVEDLLDMSRMLAGKLRLETRPMDLASVAEEAIAAVRPAAEARQIELHPALGQGGV 157 Query: 455 VIASKDLLGQVWENLLSNAVKFSKKAERAVVRVSSTSTPEEVIYQVQDNGIGFDMRYAHK 514 ++ L QV NLL NA+KF+ + R V +S + V +V DNG G + Sbjct: 158 ILGDPARLQQVVWNLLMNAIKFTPRQGR--VSLSLRQHDDTVALEVSDNGRGIKGEFLGS 215 Query: 515 LFGTFQRLHRQEDY---------AGTGIGLALVQRIVVRHGGRV-WAEGSPGEGASFYFA 564 +F F QED +G G+GLA+ + +V HGG++ A PG GA+F Sbjct: 216 VFDRFT----QEDVSTGSTGSTASGLGLGLAIARHLVELHGGQIRAASAGPGMGAAFTVT 271 Query: 565 LP 566 LP Sbjct: 272 LP 273 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 79.0 bits (193), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 75/276 (27%), Positives = 123/276 (44%), Gaps = 43/276 (15%) Query: 327 EKEIEELNASLKRQTR---------------------HLSNINKELESFSYSVSHDLRAP 365 + E+EE NA L+ QT+ L N+ F ++SH+LR P Sbjct: 430 QAELEETNAQLEEQTQLLELQKDDLAKTQVVLVEKATELERANQYKSEFLANMSHELRTP 489 Query: 366 LRAISGYAMMVEEDYAKELDAKGQEMLRVIRRNVGKMDALISDLLKLSKSATDEL--TPG 423 L + A ++ ++ L + + I + ALI+D+L LSK +L P Sbjct: 490 LNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGNDLLALINDILDLSKMEAGKLDIVPE 549 Query: 424 HFSMQDQVEQCIAGL----RRENDSVKFVVDPLEDVIASKDL--LGQVWENLLSNAVKFS 477 F + ++ A +++ K V+P D+ LGQ+ +NLLSNA+KF+ Sbjct: 550 SFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFT 609 Query: 478 KKAERAVVRVSSTSTPE---EVIYQVQDNGIGFDMRYAHKLFGTFQR----LHRQEDYAG 530 +K E VS P+ + + V+D GIG +F F++ HR+ Y G Sbjct: 610 EKGE-----VSLRIYPDAGGRIAFAVRDTGIGIGEDQHQFIFEAFRQADGSTHRK--YGG 662 Query: 531 TGIGLALVQRIVVRHGGRVWAEGSPGEGASFYFALP 566 TG+GL++ + + GG + GEG+ F +LP Sbjct: 663 TGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 54/178 (30%), Positives = 94/178 (52%), Gaps = 12/178 (6%) Query: 29 VQHTNTVLQRLEETHSTLRNLVANARGYALSGRQEYLLGFEADRSLLYELLNGLRALQLD 88 V HT V ++L+ STL++ + RGY L+G + YL + R+ L + LRAL D Sbjct: 51 VSHTMKVREQLQSLVSTLKDAETSQRGYLLTGTESYLSPYNQARAALPGEIARLRALLAD 110 Query: 89 NQEQLDRLTHLSSSIAQRI-ELARTLIEADRRVRIDNAY-------EHGRDLSEDIRKQF 140 N +QL RL+ + ++ ELA T+ D R + D A + G+ + +R Sbjct: 111 NPDQLQRLSQIEGITNDKLQELAETI---DLRRKGDAAAALAVVQSDRGKMSMDRVRTLA 167 Query: 141 VALKQVEQTLLSERSADTRESIRWTLAAILGGSFVSVVILIAVYLRLKRETMRRREAQ 198 ++ E+ LL+ R +D R+++ ++ A GGS + +V+++A + R+ R+RE Q Sbjct: 168 REMEGEERDLLAARQSDWRDAVSFSTAVAWGGSGLLLVLIVAAAMTTSRD-YRQREKQ 224 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 74/253 (29%), Positives = 116/253 (45%), Gaps = 26/253 (10%) Query: 329 EIEELNASLKRQTRHLSNINKELESFSYSVSHDLRAPLRAISGYAMMVEEDYAKELDAKG 388 +I L L + L+++ E F VSH+L+ PL A+ A ++++ A +L Sbjct: 248 DIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLTDSQ 307 Query: 389 QEMLRVIRRNVGKMDALISDLLKLSKSATD--ELTPG--------HFSMQDQVEQCIA-- 436 +E+ R++R+N + I DLL+ ++ + D + P H + DQ Q +A Sbjct: 308 REIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLARE 367 Query: 437 -GLRRENDSVKFVVDPLEDVIASKDLLGQVWENLLSNAVKFSKKAERAVVRVSSTSTPEE 495 + E + VVDP + I V NLLSNAV+FS + +R + P Sbjct: 368 LKVEIEGAARTVVVDPEKMAI--------VLANLLSNAVRFSPQG--GFIRFLLSEAPGL 417 Query: 496 VIYQVQDNGIGFDMRYAHKLFGTFQRLHRQEDYA--GTGIGLALVQRIVVRHGGRVWAEG 553 V + D G G A ++F F + Q A G GIGL++V+ V H G+V+ Sbjct: 418 VRVECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVP 477 Query: 554 SPGEGASFYFALP 566 G GA F LP Sbjct: 478 REG-GAHFRIELP 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,682,543 Number of extensions: 67459 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 362 Number of HSP's successfully gapped: 30 Length of query: 570 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 480 Effective length of database: 1,193,720 Effective search space: 572985600 Effective search space used: 572985600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)