BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0141.0017.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 731 0.0 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 128 1e-30 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 113 4e-26 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 86 5e-18 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 62 1e-10 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 731 bits (1888), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/474 (75%), Positives = 404/474 (85%), Gaps = 2/474 (0%) Query: 13 MEEDVILMVRELKRGGFEPAWQRVD-TGPDMEAALRDKWDIVISDYSMPMFSAVDALELV 71 MEED LMVRELKRGGFEPAW+RVD + A WDIVISDYSMPMFS V+AL+LV Sbjct: 1 MEEDAFLMVRELKRGGFEPAWERVDDEQALLAALQAQPWDIVISDYSMPMFSGVEALKLV 60 Query: 72 KRQTDQIPFIIVSGAIGEQTAVEVMKAGAQDYFLKSAITRLPIAVERELRDAQVRRKQRA 131 K DQ PFIIVSG IGEQTAVEVMKAGAQDYFLKSAI RLP AV+RELRDAQ RRKQRA Sbjct: 61 KAHNDQTPFIIVSGVIGEQTAVEVMKAGAQDYFLKSAIGRLPHAVDRELRDAQARRKQRA 120 Query: 132 SAAALREMQARFYAFMSAAPMPAWIKDADLRYLYVNPAQSAFFGMTPNDMIGLSDYDIMS 191 SA ALREMQARF AFM+AAPMP WIKDA LRY YVNPAQSAF+GMTP DM GL+D ++MS Sbjct: 121 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPGDMDGLTDLELMS 180 Query: 192 GGAAQNAQACDRKVLDSNREMHTQETVFDADHNPKIMDMVRFPIGSDGK-ILAAGLAMDV 250 GAA +Q DRKVL+ E+HTQETVFD +HN +I+D+VRFPI +GK + AGLA+DV Sbjct: 181 SGAAAASQENDRKVLEQKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDV 240 Query: 251 TERVNSQKELEEAMQRQKMLASRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKT 310 TER S+++LE A+QRQ++L+SRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKK Sbjct: 241 TEREQSRRDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKM 300 Query: 311 GSLEGPSLQNGIDIVAAVLSQVRSLSLDLRPPQLDNLGLIATLRSYVENKSTLAGVKGWF 370 GSLEGPSLQNGIDIV AVL+QVRSLSLDLRPPQLDNLGLIA LRSYVE KS LA V GWF Sbjct: 301 GSLEGPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWF 360 Query: 371 ESSPLSVELHHDIENTCFRIVQEAVTNILRHAQADNIWVKVSQRQDQLSVSIRDDGKGFD 430 ESS +LHHDIENT FRIVQEAVTNILRHA+ NIWVK+ ++ DQ+ +SIRDDG+GFD Sbjct: 361 ESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFD 420 Query: 431 LEKARSNAISGTSFGLLNMEERAVLVGGTMDIISAPGKGVEIILQLPVTPVMRR 484 +E+ARSNAISGTSFGLLNMEERAVLVGG+M+I SAPG G EI++ LPV P++RR Sbjct: 421 IERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPIVRR 474 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 128 bits (322), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 106/386 (27%), Positives = 185/386 (47%), Gaps = 43/386 (11%) Query: 122 DAQVRRKQRASAAALREMQARFYAFMSAAPMPAWIKDADLRYLYVNPAQSAFFGMTPNDM 181 D++ + R +A L E A A + P ++K+ ++ NPA G T + Sbjct: 301 DSEYGERTR-NAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEET 359 Query: 182 IGLSDYDI-MSGGAAQNAQACDRKVLDSNREMHTQETVFDADHNPK---IMDMVRFP-IG 236 + S ++ + A DR+V+ S + ++T+ H P+ + P G Sbjct: 360 MYRSSRELFLVPEEADTIDRDDRRVMASGKPEKLEQTL----HLPEGVVTFQTAKVPWFG 415 Query: 237 SDGKILAA-GLAMDVTERVNSQKELEEAMQRQKMLASRVIE--------------VQERE 281 DG + G++ D+TER ++ EL QR+ L VI+ V+E E Sbjct: 416 KDGSVQGVIGISTDITERKQAEDELR---QRESQLEKTVIQRTALLRELTNHLETVREEE 472 Query: 282 RQHLARGLHDDVGQSLTALKINLETIKKTGSLEGPSLQNGID-------IVAAVLSQVRS 334 ++ +AR LHD++G SLTAL ++LE + + P + D ++ ++++ R Sbjct: 473 KRAIARELHDNMGASLTALSMHLEGVYQIL----PPDEKWADRKVRMQGLMKSLVATTRR 528 Query: 335 LSLDLRPPQLDNLGLIATLRSYVENKSTLAGVKGWFESSPLSVELHHDIENTCFRIVQEA 394 + +LRP LD GL A + ++ G+ VE+ H++E +R++QE Sbjct: 529 IQTELRPNTLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEM 588 Query: 395 VTNILRHAQADNIWVKVSQRQDQLSVSIRDDGKGFDLEKARSNAISGTSFGLLNMEERAV 454 + N+ +HA+A + V + +D +++++RDDG G E+ R N ++GL + ERA Sbjct: 589 LNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGIP-EERRDNH---KTYGLRGLRERAT 644 Query: 455 LVGGTMDIISAPGKGVEIILQLPVTP 480 GG +DI S PGKG I + LP+ P Sbjct: 645 FFGGEVDIRSTPGKGALITISLPIRP 670 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 113 bits (282), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 85/240 (35%), Positives = 127/240 (52%), Gaps = 17/240 (7%) Query: 256 SQKELEEAMQRQKMLASRVIEVQERERQHLARGLHDDVGQSLTALKINLET--IKKTGSL 313 S+ + +A RQ LA RV+ QE ER L+R LHD + Q L ++K+ +E ++ G+ Sbjct: 226 SEHRVADAKLRQ--LAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTP 283 Query: 314 E-GPSLQNGIDIVA----AVLSQVRSLSLDLRPPQLDNLGLIATLRSYVEN--KSTLAGV 366 E + + G + A VL++VR +S DLRP LD+LGL A L + AGV Sbjct: 284 EQAATARAGFEKTATQINGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGV 343 Query: 367 KGWFESSPLS---VELHHDIENTCFRIVQEAVTNILRHAQADNIWVKVSQRQDQLSVSIR 423 + FE+ + L + FRI QEA+TNI RHA A I V++ Q + L + ++ Sbjct: 344 EIVFENQAQADDGATLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQ 403 Query: 424 DDGKGFDLEKARSNAISGTSFGLLNMEERAVLVGGTMDIISAP-GKGVEIILQLPVTPVM 482 D+G GFDL + ++ G GL NM ER VGG + I S+P G + ++ P + Sbjct: 404 DNGHGFDLGQVDDHSRHG--IGLRNMRERIEAVGGELGIDSSPAGTSISAVIARASLPSL 461 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 86.3 bits (212), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 69/231 (29%), Positives = 115/231 (49%), Gaps = 23/231 (9%) Query: 258 KELEEAMQRQKMLASRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKTGSLE--- 314 + L A++ Q+++A ER LA+GLHD + Q L L + ++ ++ + + + Sbjct: 423 RHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLD 482 Query: 315 -----GPSLQNGIDIVAAVLSQVRSLSLDLRPPQLDNLGLIATLRSYVENKSTLAGVKGW 369 P L+ G V VR L L+ R +L + L + +R ++ +G++G Sbjct: 483 EIRDIAPLLRTG---VQENYEDVRELLLNFR-TRLQDSNLDSEMRKALDKLQRQSGLQGQ 538 Query: 370 F----ESSPLSVELHHDIENTCFRIVQEAVTNILRHAQADNIWVKVSQRQDQLSVSIRDD 425 + PL+ E + I+QEA++NI +HA+A + + V RQD +++ DD Sbjct: 539 ITLTGDGPPLAPEQQLQV----LFILQEALSNIRKHARAQQVQISVDNRQD-FRMTVSDD 593 Query: 426 GKGFDLEKARSNAISGTSFGLLNMEERAVLVGGTMDIISAPGKGVEIILQL 476 G GF LE AR+ + GL M+ERA +G ++I S PG G I L L Sbjct: 594 GHGFTLESARAKGLE--HVGLRIMQERAERLGANLEIDSQPGHGTRIALTL 642 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 56/211 (26%), Positives = 97/211 (45%), Gaps = 7/211 (3%) Query: 274 VIEVQERERQHLARGLHDDVGQSLTALKINLETIKKTGSLEG-----PSLQNGIDIVAAV 328 I ++E+ER+ +AR LHDD+G L + + L +++ + +G ++ Sbjct: 147 AIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQA 206 Query: 329 LSQVRSLSLDLRPPQLDNLGLIATLRSYVENKSTLAGVKGWFESSPLSVELHHDIENTCF 388 ++ + S+ +L PP ++ GL+ L+ + + P + + Sbjct: 207 MASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILGLL 265 Query: 389 RIVQEAVTNILRHAQADNIWVKVSQRQDQLSVSIRDDGKGFDLEKARSNAISGTSFGLLN 448 RI++EA +N RH +A + V + RQ +L + I DDG G A+ A GL N Sbjct: 266 RILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVA-PRHGHGLRN 324 Query: 449 MEERAVLVGGTMDIISAPGKGVEIILQLPVT 479 M ERA +GG + I + GV I +PV Sbjct: 325 MRERAQALGGELFIEAGQDGGVVIRTLIPVA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,431,909 Number of extensions: 56961 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 288 Number of HSP's successfully gapped: 6 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)