BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0140.0009.p
         (259 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo...   213   1e-56
gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo...   125   5e-30

>gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1
           component protein [Herbaspirillum seropedicae SmR1]
          Length = 893

 Score =  213 bits (543), Expect = 1e-56,   Method: Compositional matrix adjust.
 Identities = 113/165 (68%), Positives = 133/165 (80%), Gaps = 3/165 (1%)

Query: 95  KAGAQVRLLAAGPMLKEALSAARTLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPG 154
           K  AQ RLL +GP+LKEA++AA  LQ+ + + AEVWSVTS+TELARDG+AA+R  RL   
Sbjct: 732 KPQAQARLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELARDGVAAQRAQRLAGS 791

Query: 155 AKNDLPYVTQQLQAGKAPVIAVSDYVRTLSESIRAYALPARYVTLGTDGFGRSDTRANLR 214
           A   LPYVTQQLQ    PVIAVSDY+RTL ESIRA+ +PA YVTLGTDGFGRSDTR NLR
Sbjct: 792 AA--LPYVTQQLQGSAVPVIAVSDYIRTLPESIRAF-VPAPYVTLGTDGFGRSDTRVNLR 848

Query: 215 DFFEVDARWIAYTALTEVFGADAARLKDAAARLGVDLDKPLSTSV 259
           DFFE+DARWIAYTAL EV+G D ARL++AA  LG+D  KPLS++V
Sbjct: 849 DFFEIDARWIAYTALCEVWGDDRARLQEAARTLGIDTAKPLSSTV 893



 Score =  109 bits (272), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 59/90 (65%), Positives = 71/90 (78%), Gaps = 2/90 (2%)

Query: 2   RLLAAGPMLKEALSAARTLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPGAKNDLP 61
           RLL +GP+LKEA++AA  LQ+ + + AEVWSVTS+TELARDG+AA+R  RL   A   LP
Sbjct: 738 RLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELARDGVAAQRAQRLAGSAA--LP 795

Query: 62  YVTQQLQAGKAPVIAVSDYVRTLSESIRAY 91
           YVTQQLQ    PVIAVSDY+RTL ESIRA+
Sbjct: 796 YVTQQLQGSAVPVIAVSDYIRTLPESIRAF 825


>gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1
           component protein [Herbaspirillum seropedicae SmR1]
          Length = 898

 Score =  125 bits (313), Expect = 5e-30,   Method: Compositional matrix adjust.
 Identities = 68/162 (41%), Positives = 99/162 (61%), Gaps = 8/162 (4%)

Query: 99  QVRLLAAGPMLKEALSAARTLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPGAKND 158
           +V+LL +G +L+E ++AA  L++ + V A+VWS  SFT LARDG   ER   L P     
Sbjct: 734 RVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDVERWNMLHPAEAPK 793

Query: 159 LPYVTQQLQAGKAPVIAVSDYVRTLSESIRAYALPAR-YVTLGTDGFGRSDTRANLRDFF 217
             Y  Q L+  + P++  +DY+RT +E +RA+    R Y  LGTDG+GRSDTRA LR+FF
Sbjct: 794 KSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGYGRSDTRAKLREFF 853

Query: 218 EVDARWIAYTALTEVFGADAARLK-----DAAARLGVDLDKP 254
           EV+  ++   AL  +  AD  ++K      A A+ G+D +KP
Sbjct: 854 EVNRYFVTVAALKSL--ADEGKIKPEVVAQAIAKYGIDPNKP 893



 Score = 77.0 bits (188), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 37/91 (40%), Positives = 56/91 (61%)

Query: 1   VRLLAAGPMLKEALSAARTLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPGAKNDL 60
           V+LL +G +L+E ++AA  L++ + V A+VWS  SFT LARDG   ER   L P      
Sbjct: 735 VQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDVERWNMLHPAEAPKK 794

Query: 61  PYVTQQLQAGKAPVIAVSDYVRTLSESIRAY 91
            Y  Q L+  + P++  +DY+RT +E +RA+
Sbjct: 795 SYFEQSLEGSEGPIVVSTDYMRTYAEQVRAF 825


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.130    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 725,735
Number of extensions: 27959
Number of successful extensions: 136
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 132
Number of HSP's successfully gapped: 4
Length of query: 259
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 176
Effective length of database: 1,226,865
Effective search space: 215928240
Effective search space used: 215928240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 129 (54.3 bits)