BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0140.0005.p (614 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309830|ref|YP_003773922.1| ATP-dependent DNA helicase [Her... 1087 0.0 gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Her... 60 5e-10 gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Her... 57 4e-09 >gi|300309830|ref|YP_003773922.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 618 Score = 1087 bits (2810), Expect = 0.0, Method: Compositional matrix adjust. Identities = 516/600 (86%), Positives = 557/600 (92%), Gaps = 1/600 (0%) Query: 13 QGKALHILETVFGYPSFRGQQADIVQHVVQGGDALVLMPTGGGKSLCYQVPALVRDGVGV 72 Q +ALH+LETVFGY SFRG Q +IVQHV GGDALVLMPTGGGKSLCYQVPAL+R G GV Sbjct: 14 QERALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQVPALLRQGTGV 73 Query: 73 VVSPLIALMQDQVDALAEVGVRAAFLNSTQTFDEAMQIERRVRSGDIDLLYVAPERLMTP 132 V+SPLIALMQDQVDAL EVGVRAAFLNSTQ+FDEAMQIERR+R GD+DLLYVAPERLMTP Sbjct: 74 VISPLIALMQDQVDALDEVGVRAAFLNSTQSFDEAMQIERRLRQGDLDLLYVAPERLMTP 133 Query: 133 RCLDLLEAAHIALFAIDEAHCVSQWGHDFRPEYIKLSVLHERFPNVPRIALTATADQQTR 192 RCLDLLE+A I+LFAIDEAHCVSQWGHDFRPEYIKLSVLHERFP VPRIALTATADQQTR Sbjct: 134 RCLDLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSVLHERFPQVPRIALTATADQQTR 193 Query: 193 EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIESEHPDDAGVVYCLSRKK 252 EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFI+SEHP+DAG+VYCLSRKK Sbjct: 194 EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKSEHPEDAGIVYCLSRKK 253 Query: 253 VEETAEFLKQSGINALPYHAGMDYAKRTANQARFLREDKIVMVATIAFGMGIDKPDVRFV 312 VEETA+FL+ GINAL YHAGMDYA+RTANQARFLREDKIVMVATIAFGMGIDKPDVRFV Sbjct: 254 VEETADFLRSEGINALAYHAGMDYAQRTANQARFLREDKIVMVATIAFGMGIDKPDVRFV 313 Query: 313 AHLDLPKSIEGYYQETGRAGRDGLPANAWMAYGLQDVVQQRRMISESEADETFKRVLGIK 372 HLDLPKS+EGYYQETGRAGRDGLPA+AWMAYGLQDVVQQRRMI ES+ADETFKRVLG K Sbjct: 314 CHLDLPKSVEGYYQETGRAGRDGLPADAWMAYGLQDVVQQRRMIDESDADETFKRVLGTK 373 Query: 373 LDAMLGLCETLHCRRVRLLDYFGQHGQQACGNCDICLTPPVSFDGTLMAQKLLSTVYRVE 432 L+AML LCETLHCRRV LLDYFG+ CGNCD CLTPPVSFD T+ QKLLSTVYRVE Sbjct: 374 LNAMLALCETLHCRRVHLLDYFGEKS-APCGNCDTCLTPPVSFDATVEVQKLLSTVYRVE 432 Query: 433 QRFAAGHVLDVLRGIDGDRVKQWRHDQLSVFGIGSEYGEAEWRAVLRQVIALGLLTVDHE 492 QRFA GHV++VLRGIDG+RVKQWRHDQLSVFG+GS+ GEAEWRA+LRQVIALGLLTVD + Sbjct: 433 QRFAPGHVIEVLRGIDGERVKQWRHDQLSVFGVGSDRGEAEWRAILRQVIALGLLTVDAD 492 Query: 493 NYSSLKLTEEAKPVLRGEQKIQLRQYQKPVKPKRTSSKSNYAETDLSTEEQGIFDKLRWW 552 NYS+LKLTE A+PVLRGEQ +QLR+YQKP K KR+S +S++ ETDLSTEEQ +F+KLRWW Sbjct: 493 NYSALKLTEAARPVLRGEQAVQLRRYQKPEKAKRSSQRSSFVETDLSTEEQALFEKLRWW 552 Query: 553 RVETARKHNVPAYVIFHDATMREIAKARPSSLDDLRGVTGVGEKKLETYGAEIIALIQEL 612 RVETAR HNVPAYVIFHDATMREIAKARP SLDDLR VTGVGEKKLETYGA+IIALI E+ Sbjct: 553 RVETARAHNVPAYVIFHDATMREIAKARPQSLDDLRHVTGVGEKKLETYGAQIIALIAEM 612 >gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 60.1 bits (144), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 83/342 (24%), Positives = 131/342 (38%), Gaps = 51/342 (14%) Query: 25 GYPSFRGQQADIVQHVVQGGDALVLMPTGGGKSLCYQVPALVRDGVGVVVSPLIALMQDQ 84 GY S QA V V+ GGD L TG GK+ + +P L R L A+ + + Sbjct: 20 GYTSPTPIQAQAVPAVLSGGDLLAGAQTGTGKTAGFTLPLLQR---------LSAMPRQK 70 Query: 85 VDALAEVGVRAAFLNSTQTFDEAMQIERRVR--------------------------SGD 118 ++ V +RA L T+ A Q+E VR Sbjct: 71 ING--HVPIRALILAPTREL--AAQVEESVRQYGKYLPLTSACIFGGVGINPQITLLKRG 126 Query: 119 IDLLYVAPERLMTPRCLDLLEAAHIALFAIDEAHCVSQWG--HDFRPEYIKLSVLHERFP 176 +D+L P RL+ + HI + +DEA + G D R VL P Sbjct: 127 VDILVATPGRLLDHMQQGTVNLQHIQILVLDEADRMLDMGFIRDIR------KVLAALPP 180 Query: 177 NVPRIALTAT-ADQQTR--EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDF 233 + +AT AD+ + + +++ L + + S+ + + V++ L Sbjct: 181 KRQNLLFSATFADEIKKLADSLLNNPALIEVARRNSTVEVIAQKIHPVDRDKKHPMLAHL 240 Query: 234 IESEHPDDAGVVYCLSRKKVEETAEFLKQSGINALPYHAGMDYAKRTANQARFLREDKIV 293 I++ H +V+ ++ + E L + GI A+ H + RT A F Sbjct: 241 IKT-HQWKQVLVFTRTKHGANKLVEQLGRDGITAMAIHGNKSQSARTKALAEFKDGKLTA 299 Query: 294 MVATIAFGMGIDKPDVRFVAHLDLPKSIEGYYQETGRAGRDG 335 ++AT GID + V + DLP E Y GR GR G Sbjct: 300 LIATDIAARGIDIDQLPHVVNYDLPNVPEDYVHRIGRTGRAG 341 >gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 89/355 (25%), Positives = 132/355 (37%), Gaps = 44/355 (12%) Query: 22 TVFGYPSFRGQQADIVQHVVQGGDALVLMPTGGGKSLCYQVPALVRDGVGVVVSPLIALM 81 T GY S Q + + G D LV TG GK+ + +P+L + +P A Sbjct: 17 TEAGYTSPTPVQEKAIPAAISGQDLLVSSQTGSGKTAAFMLPSL-HQLADLPPAPQAART 75 Query: 82 QDQ------------------VDALAEVGVRAAFLNSTQTFDEAMQIERRVRSGDI---- 119 +Q L R L T D+ RRVR I Sbjct: 76 PNQERQASRARGERPRYQPAQPKMLVLTPTRELALQVTTATDKYGAYMRRVRVVSILGGM 135 Query: 120 ------DLLYVAPERLM-TP-RCLDLLEAAHI-----ALFAIDEAHCVSQWGHDFRPEYI 166 LL PE L+ TP R +D +E+ I + +DEA + G E I Sbjct: 136 PYPKQMQLLSRNPEILVATPGRLIDHMESGKIDFSQLQILVLDEADRMLDMGFIDDIEKI 195 Query: 167 KLSVLHERFPNVPRIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNI--RYQIVEKA 224 + R + L + + L ++ A SS NI R V+ Sbjct: 196 VAATPATRQTMLFSATLDGVVGNMAKRITNNPLTIQIAS---SSTRHENIMQRVHFVDDL 252 Query: 225 NGRKQLLDFIESEHPDDAGVVYCLSRKKVEETAEFLKQSGINALPYHAGMDYAKRTANQA 284 + + +LLD + + D V++ +++ + A+ L +G A H M R Sbjct: 253 SHKNRLLDHLLRDTSIDQAVIFTATKRDADTIADRLNIAGFAAAALHGDMHQGARNRTLN 312 Query: 285 RFLREDKIVMVATIAFGMGIDKPDVRFVAHLDLPKSIEGYYQ---ETGRAGRDGL 336 R ++VAT GID P + V + DLPK E Y TGRAGR+G+ Sbjct: 313 SLRRGQVRMLVATDVAARGIDVPGITHVFNYDLPKFAEDYVHRIGRTGRAGRNGV 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,934,442 Number of extensions: 84078 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 4 Length of query: 614 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 523 Effective length of database: 1,188,985 Effective search space: 621839155 Effective search space used: 621839155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)