BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0139.0001.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 105 5e-24 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 105 6e-24 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 99 3e-22 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 96 3e-21 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 92 4e-20 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 49/119 (41%), Positives = 75/119 (63%), Gaps = 1/119 (0%) Query: 1 MVYIGRWFPTSYRARVTGIFMVAIPLSGLIGSPISGVILDGMNGVAGLRGWQWMFIIEAI 60 ++Y+ W P++YRAR+ +FMVAIP + + SP+S ++L M+G+ GLRGW W+F++E + Sbjct: 131 VLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFMLEGL 189 Query: 61 PALLLGLACLRMLADGPSEVSWLSAGEKQRLELIMTRERQALDAGGHYKLSAAFTNPAV 119 P ++LGL CL L D P + WLS ++Q L M +E + A H L F+ P V Sbjct: 190 PTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHV 248 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 105 bits (261), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 50/115 (43%), Positives = 80/115 (69%), Gaps = 1/115 (0%) Query: 2 VYIGRWFPTSYRARVTGIFMVAIPLSGLIGSPISGVILDGMNGVAGLRGWQWMFIIEAIP 61 +Y +WFP ++R + T F++ IP++ +IGSP+SG +++ ++G+ G +GWQ + ++EA+P Sbjct: 141 LYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEALP 199 Query: 62 ALLLGLACLRMLADGPSEVSWLSAGEKQRLELIMTRERQALDAGGHYKLSAAFTN 116 A+LLG+ CL +L D P++ WLSA EKQ LE ++ E+ L A KL AFTN Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTN 254 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 48/116 (41%), Positives = 69/116 (59%), Gaps = 2/116 (1%) Query: 1 MVYIGRWFPTSYRARVTGIFMVAIPLSGLIGSPISGVILDGMNGVAGLRGWQWMFIIEAI 60 ++Y+ WFP RARV G+FM + ++G +G PI+G+I+ GM GV L WQW+FI+E I Sbjct: 141 VLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGI 200 Query: 61 PALLLGLACLRMLADGPSEVSWLSAGEKQRLELIMTRERQALDAGGHYKLSAAFTN 116 P++L G A L L + P+ WLS EK + ++ E A G H+ A TN Sbjct: 201 PSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSA--PGKHFSFKDACTN 254 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 95.9 bits (237), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 51/121 (42%), Positives = 73/121 (60%), Gaps = 3/121 (2%) Query: 1 MVYIGRWFPTSYRARVTGIFMVAIPLSGLIGSPISGVILDGMNGVAGLRGWQWMFIIEAI 60 ++Y+ W+P + R ++T +FM I LSG+IG P+SG I+ M GV G GWQWMFI+EAI Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 61 PALLLGLACLRMLADGPSEVSWLSAGEKQRLELIMTRERQALDAGGHYKLSAAFTNPAVL 120 P+L+LG+ + + D + WLS EK+ LE ++ E + H L A F N V Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE---HLSLGAMFANGRVW 253 Query: 121 L 121 L Sbjct: 254 L 254 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 41/92 (44%), Positives = 63/92 (68%) Query: 1 MVYIGRWFPTSYRARVTGIFMVAIPLSGLIGSPISGVILDGMNGVAGLRGWQWMFIIEAI 60 ++Y+ WFP+ RA+V +FM IP++G++G+P+SG I+D + GL GWQWMF+IEAI Sbjct: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 Query: 61 PALLLGLACLRMLADGPSEVSWLSAGEKQRLE 92 PA+L+G+A + L + WL+ EK L+ Sbjct: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQ 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 698,448 Number of extensions: 25696 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 9 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)