BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0138.0031.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 769 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 105 8e-24 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 101 1e-22 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 92 9e-20 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 84 2e-17 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 769 bits (1985), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/381 (95%), Positives = 374/381 (98%) Query: 1 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSIMHYTQQ 60 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKS+MHYTQQ Sbjct: 28 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQ 87 Query: 61 KNIQGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV 120 K I+GVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV Sbjct: 88 KKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV 147 Query: 121 CDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLIIFAD 180 CDEQQGWQPDIADI+KKITPNTKAIVVINPNNPTGALYS EVLKE++E+ARQHQLII AD Sbjct: 148 CDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILAD 207 Query: 181 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHAKDYIEG 240 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHA+DYIEG Sbjct: 208 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEG 267 Query: 241 LNMLASMRLCANAPGQYAIQTALGGYQSINDLVAPSGRLAKQRDLAHKLLTDIPGVTCVK 300 LNMLASMRLCANAPGQ+AIQTALGGYQSINDLV P GRL KQRDLAH+LLTDIPGV+CVK Sbjct: 268 LNMLASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGVSCVK 327 Query: 301 PKSALYMFPRLDPDIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD 360 PKSALYMFPRLDP+IYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD Sbjct: 328 PKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD 387 Query: 361 LTESMGRIAHFLEAYRRRHGT 381 LTESMGRIAHFLE YRRRHGT Sbjct: 388 LTESMGRIAHFLEGYRRRHGT 408 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 105 bits (261), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 93/369 (25%), Positives = 169/369 (45%), Gaps = 18/369 (4%) Query: 2 EEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR--NMGNASGYTDSKGLFAPRKSIMHYTQ 59 G II +++GN P IV+ ++ + GY+ SKG+ R++I H+ + Sbjct: 63 RRRGEDIIDMSMGNPDG---ATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYR 119 Query: 60 QKNIQGVTID-DIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVH 118 + D + + G+ E + + A L+ GD VLVP+P YP+ ++G Sbjct: 120 SRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRS 179 Query: 119 YVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLIIF 178 + ++ ++ P K +++ P+NPT E + +V++AR+HQ+++ Sbjct: 180 VRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVV 239 Query: 179 ADEIYDKVLYDGNTHTSLASL--ADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHAKD 236 D Y + +DG S+ + A +V + F LSK+Y G+R G+MV + A Sbjct: 240 HDLAYADITFDGWKAPSIMQVPGAREVA-VEFFTLSKSYNMAGWRIGFMVGNARLVAALA 298 Query: 237 YIEGLNMLASMRLCANAPGQYAIQTALGGYQSINDLVAPSGRLAKQRDLAHKLLTDIPGV 296 I+ + S P Q A AL G QS + + ++R++ K L + G Sbjct: 299 RIKSYHDYGSF-----TPVQVAAIAALEGDQSCVEEI--RANYERRRNVLVKGLHEA-GW 350 Query: 297 TCVKPKSALYMFPRLDPDIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLP 356 PK+++Y++ R+ P+ Y EFA LL + KV + G GF ++ R + Sbjct: 351 MVDVPKASMYIWARI-PEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDEYVRFALIE 409 Query: 357 NTDDLTESM 365 N + +++ Sbjct: 410 NESRIRQAI 418 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 101/384 (26%), Positives = 177/384 (46%), Gaps = 27/384 (7%) Query: 1 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSIM-HYT 58 +E++G +I + IG F AP +V+ R M YT + GL R +I HY Sbjct: 28 LEQQGRHLIHMGIGEP---DFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHY- 83 Query: 59 QQKNIQGVTI--DDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTP 116 +++ G+ I + I + GAS ++++ AL+ EVL+P P YP V+ G Sbjct: 84 --RSVYGLEIAPERIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRA 141 Query: 117 VHYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLI 176 + +Q +R+ T+ +++ +P+NPTG + L+ +V RQ Sbjct: 142 KLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGF 201 Query: 177 IFADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHAKD 236 DEIY + Y+G ++L SL DDV+ I N SK + G+R GW+V+ Sbjct: 202 TIVDEIYQGLSYEGAPFSAL-SLGDDVVVI--NSFSKYFNMTGWRLGWLVLP------PA 252 Query: 237 YIEGLNMLA-SMRLCANAPGQYAIQTALGGYQSINDLVAPSGRLAKQRDLAHKLLTDIPG 295 + + LA ++ +CA++ Q+A A Q++ ++RD L + Sbjct: 253 LVPQVEKLAQNLLICASSIAQHA-AVACFTPQTLALYEERKAEFKRRRDYIVPALESLGF 311 Query: 296 VTCVKPKSALYMFPRLDPDIYPIKDDQ-EFAYELLAEEKVLIVQGTGFNCPTPDHF-RVV 353 V P A Y++ D + DD + + ++L E V++V G F T + R+ Sbjct: 312 TVPVMPDGAFYVY----ADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARRYIRLS 367 Query: 354 FLPNTDDLTESMGRIAHFLEAYRR 377 + + ++L E++ R+ F E R+ Sbjct: 368 YATSMENLQEAVARLRVFFEKRRK 391 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 91.7 bits (226), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 59/197 (29%), Positives = 100/197 (50%), Gaps = 13/197 (6%) Query: 68 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE--QQ 125 + +I + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV +V E Q Sbjct: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV-FVQMEVGAQ 147 Query: 126 GWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLIIFADEIYDK 185 G+ ++ +TP T+ I+V P+NPTG++ + L ++ R ++I +DE+Y+ Sbjct: 148 GYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEH 207 Query: 186 VLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHAKDYIEGLN 242 ++YDG H SLA LA+ FI + K Y G++ G++ + N Sbjct: 208 MVYDGQPHESLARHPELAERS-FIN-SSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFN 265 Query: 243 MLASMRLCANAPGQYAI 259 + N P QY + Sbjct: 266 VFT-----VNTPVQYGL 277 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 87/387 (22%), Positives = 167/387 (43%), Gaps = 35/387 (9%) Query: 3 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSIMHYTQQKN 62 + ++ I L IG P I Q +I N+ + Y + G A R +I + Q++ Sbjct: 43 DPAYRPISLGIGEPK---HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRY 99 Query: 63 --IQGVTIDDIYLGNGASELIVMSVNALLNT---GDEVLVPSPDYPLWTAAVSLSGGTPV 117 + +I NG+ E + +++ G V+ P+P Y ++ + L+G P Sbjct: 100 DIPRPDPATEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPY 159 Query: 118 HYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLII 177 D ++ + PD + + + + + + V +P NPTGA+ + E KEL ++ ++ +I Sbjct: 160 FVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVI 219 Query: 178 FADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKK 232 +DE Y ++ ++ G + D I+F+ LSK G R+G+ V+G+ Sbjct: 220 ASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGF--VAGDAA 277 Query: 233 HAKDYIEGLNMLASMRLCANAPGQYAIQTA-LGGYQSINDLVAPSGRLAKQRDLAHKLLT 291 K ++ + + A +P IQ A + +Q + + + LL Sbjct: 278 ILKKFL----LYRTYHGAAMSP---VIQAASVLAWQDETHVQGNIAKYVTKFSQVTPLLQ 330 Query: 292 DIPGVTCVKPKSALYMFPRLDPDIYPIKDDQEFAYELLAEEKVLIVQGT-------GFNC 344 ++ + P +A Y++ ++D + D EFA L AE V ++ G+ G N Sbjct: 331 EV--LEVALPDAAFYLWAKVDKLVN--ISDTEFAQRLYAEYNVTVLPGSYLAREAHGVN- 385 Query: 345 PTPDHFRVVFLPNTDDLTESMGRIAHF 371 P R+ + ++ E+ RI F Sbjct: 386 PGAGRIRMALVAEVEECLEAAQRIVAF 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,232,575 Number of extensions: 50886 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)