BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0138.0002.p (184 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 198 2e-52 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 143 9e-36 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 113 1e-26 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 112 2e-26 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 87 7e-19 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 198 bits (504), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 89/113 (78%), Positives = 106/113 (93%) Query: 1 MPQFFINRPVFAWVVAIFIILLGLIALPQLPIARYPTVAPPSVSIYATYPGATPRSMNDS 60 MP+FFI+RP+FAWVVA+FI+L G+IA+ QLPIARYP+VAPP+VSI ATYPGATP++MND Sbjct: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 Query: 61 VVSLIERELSSVKNLLYFESSSDTSGSAQITATFKPGTDPDLAQVDVQNRLAA 113 V+SLIERELS VKNLLYFESS+DTSG+AQI+ TFKPGTDP+LAQVDVQN+L A Sbjct: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKA 113 Score = 97.8 bits (242), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 48/61 (78%), Positives = 56/61 (91%) Query: 114 GMAYSVPFDTAPFVKISIQKVIVTLIEAMVLVFLVMFLFLQKVRYTLIPAIVAPIXVRYT 173 GMA+S+P++TAPFVKISIQKV+ TLIEAMVLVFLVM+LFLQ VRYT+IPAIVAPI + T Sbjct: 320 GMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQNVRYTVIPAIVAPIALLGT 379 Query: 174 L 174 L Sbjct: 380 L 380 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 143 bits (360), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 64/111 (57%), Positives = 86/111 (77%) Query: 1 MPQFFINRPVFAWVVAIFIILLGLIALPQLPIARYPTVAPPSVSIYATYPGATPRSMNDS 60 M +FFI+RPVFAWV+AIFI+L G +A+ LP+++YP +APP++ + ATYPGAT ++++DS Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 61 VVSLIERELSSVKNLLYFESSSDTSGSAQITATFKPGTDPDLAQVDVQNRL 111 V SLIE+E++ L Y ES S +G QIT TF P T+PDLA VDVQNRL Sbjct: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRL 111 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 38/60 (63%), Positives = 51/60 (85%) Query: 114 GMAYSVPFDTAPFVKISIQKVIVTLIEAMVLVFLVMFLFLQKVRYTLIPAIVAPIXVRYT 173 G+ Y++P+DT+ FVKISI++V+ TLIEA+VLVFLVMFLFLQ +RYT+IP IV P+ + T Sbjct: 320 GVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQNIRYTIIPTIVVPVALLGT 379 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 113 bits (282), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 55/112 (49%), Positives = 87/112 (77%) Query: 1 MPQFFINRPVFAWVVAIFIILLGLIALPQLPIARYPTVAPPSVSIYATYPGATPRSMNDS 60 M +FFI+RP+FAWV+AI ++L G++++ LPIA+YP +APP+++I A YPGA+ +++ D+ Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 61 VVSLIERELSSVKNLLYFESSSDTSGSAQITATFKPGTDPDLAQVDVQNRLA 112 V +IE+++ + L Y S+S++SGS IT TF+ GT+PD AQV VQN+L+ Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLS 112 Score = 73.9 bits (180), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 35/55 (63%), Positives = 43/55 (78%) Query: 114 GMAYSVPFDTAPFVKISIQKVIVTLIEAMVLVFLVMFLFLQKVRYTLIPAIVAPI 168 GM P+DT PFV+ISI++V+ TLIEA+VLVFLVM+LFLQ R TLIP I P+ Sbjct: 320 GMKVQKPYDTTPFVRISIEEVVRTLIEAVVLVFLVMYLFLQNFRATLIPTIAVPV 374 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 53/111 (47%), Positives = 84/111 (75%) Query: 1 MPQFFINRPVFAWVVAIFIILLGLIALPQLPIARYPTVAPPSVSIYATYPGATPRSMNDS 60 M +FFI+RP+FAWV+AI I+L G++++ L + +YP +APP V+I ATY GA+ +++ DS Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 61 VVSLIERELSSVKNLLYFESSSDTSGSAQITATFKPGTDPDLAQVDVQNRL 111 V +IE+++ + NL Y +SS ++GSA ++ TF GT+PD+AQ+ VQN+L Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKL 111 Score = 66.6 bits (161), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 28/51 (54%), Positives = 42/51 (82%) Query: 118 SVPFDTAPFVKISIQKVIVTLIEAMVLVFLVMFLFLQKVRYTLIPAIVAPI 168 ++ +DT PFV++S+++V+ TLIEA+VLV L+M+LFLQ +R T IPAI P+ Sbjct: 326 NIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLFLQNIRATFIPAITVPV 376 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 87.4 bits (215), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 58/156 (37%), Positives = 100/156 (64%), Gaps = 9/156 (5%) Query: 3 QFFINRPVFAWVVAIFIILLGLIALPQLPIARYPTVAPPSVSIYATYPGATPRSMNDSVV 62 +FFI+RP+FA V+++ ++L G++A+ QLPI+ YP V PPSV + A YPGA P+ + ++V Sbjct: 5 KFFIDRPIFAGVLSVLLLLGGVLAMLQLPISEYPEVVPPSVVVRAQYPGANPKVIAETVA 64 Query: 63 SLIERELSSVKNLLYFESSSDTSGSAQITATFKPGTDPDLAQVDVQNRLAAGMAYSVPFD 122 S +E +++ V+N+LY +S +++ G+ IT F+ G DPD AQ VQNR++ + Sbjct: 65 SPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVSQAL------- 117 Query: 123 TAPFVKISIQKVIVTLIEAMVLVFLVMFLFLQKVRY 158 P + +Q++ VT +++ + +V+ L RY Sbjct: 118 --PRLPEDVQRLGVTTLKSSPTLTMVVHLTSPDNRY 151 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 474,632 Number of extensions: 14894 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 74 Number of HSP's successfully gapped: 12 Length of query: 184 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 104 Effective length of database: 1,241,070 Effective search space: 129071280 Effective search space used: 129071280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)