BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0136.0029.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subu... 421 e-119 gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-bind... 204 4e-54 gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [... 127 1e-30 gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [H... 117 7e-28 >gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subunit [Herbaspirillum seropedicae SmR1] Length = 861 Score = 421 bits (1081), Expect = e-119, Method: Compositional matrix adjust. Identities = 202/226 (89%), Positives = 216/226 (95%) Query: 1 MIRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGHLTEAVRRKPYSVILLDEIEKAHPDVFN 60 MIRIDMSEFMEKHSV+RLIGAPPGYVGYEEGG+LTEAVRRKPYSVILLDEIEKAHPDVFN Sbjct: 634 MIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFN 693 Query: 61 VLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNLGSHKIQAMEDSDPAVVKMAVMAEVKLHF 120 VLLQVLDDGR+TDGQGRTVDFKNTVIVMTSNLGSHKIQ+MEDSDPA+VK+AVMAEV+ HF Sbjct: 694 VLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQSMEDSDPALVKLAVMAEVRTHF 753 Query: 121 RPEFINRVDEIVVFHALDERNIGAIAKIQLHILEERLAKMDIGLVITDAGLQKIAEAGFD 180 RPEFINRVDEIVVFHALD +NIGAIAKIQL ILE+RLAK++IGL I++AGLQKIAEAGFD Sbjct: 754 RPEFINRVDEIVVFHALDAKNIGAIAKIQLRILEQRLAKLEIGLEISEAGLQKIAEAGFD 813 Query: 181 PVYGARPLKRAIQQEIENPLSKLILQGKFGPKDVVHVSVQNGALSF 226 PVYGARPLKRAIQQEIENPLSK IL GKFGPKDVV V V +G LSF Sbjct: 814 PVYGARPLKRAIQQEIENPLSKQILAGKFGPKDVVRVDVDHGQLSF 859 >gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-binding specificity subunit [Herbaspirillum seropedicae SmR1] Length = 767 Score = 204 bits (520), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 105/217 (48%), Positives = 143/217 (65%), Gaps = 15/217 (6%) Query: 1 MIRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGHLTEAVRRKPYSVILLDEIEKAHPDVFN 60 ++R DMSE+ME+H+VSRLIGAPPGYVG+++GG LTEAV +KP++V+LLDEIEKAHPD+FN Sbjct: 519 LVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVNKKPHAVLLLDEIEKAHPDIFN 578 Query: 61 VLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNLGSHKIQ--------AMEDSDPAVVKMAV 112 +LLQV+D G LTD GR DF+N +I+MT+N G+ +Q E D Sbjct: 579 ILLQVMDHGTLTDNNGRKTDFRNVIIIMTTNAGAESLQKRTIGFTEKKEAGDE------- 631 Query: 113 MAEVKLHFRPEFINRVDEIVVFHALDERNIGAIAKIQLHILEERLAKMDIGLVITDAGLQ 172 MA++K F PEF NR+D I+ F LDE I + L LEE+L + + V +D+ + Sbjct: 632 MADIKRMFTPEFRNRLDAIISFRPLDEEIILRVVDKFLMQLEEQLHEKKVEAVFSDSLRK 691 Query: 173 KIAEAGFDPVYGARPLKRAIQQEIENPLSKLILQGKF 209 + + GFDP+ GARP+ R IQ I L+ +L GK Sbjct: 692 FLGKKGFDPLMGARPMSRLIQDLIRKALADELLFGKL 728 >gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 73/166 (43%), Positives = 111/166 (66%), Gaps = 8/166 (4%) Query: 1 MIRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGHLTEAVRRKPYSVILLDEIEKAHPDVFN 60 +I I+MSE+ E H+VS L G+PPGYVGY EGG LTEAVRR PYSV+LLDE+EKAHPDV Sbjct: 655 LITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHPDVLE 714 Query: 61 VLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNLGSHKI-------QAMEDSDPAVVKMAVM 113 + QV D G + DG+GR +DFKNT+I++TSN+ S ++ A E P + + + Sbjct: 715 LFFQVFDKGVMDDGEGREIDFKNTIIILTSNVASSQLMQACLNKSAAELPTPDELDVLIR 774 Query: 114 AEVKLHFRPEFINRVDEIVVFHALDERNIGAIAKIQLHILEERLAK 159 ++ F+P F+ R+ +++ ++ + + + I K++L + R+A+ Sbjct: 775 PQLVKAFKPAFLGRL-KVIPYYPISDEVLEQIIKLKLGRIANRIAE 819 >gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [Herbaspirillum seropedicae SmR1] Length = 889 Score = 117 bits (293), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 73/166 (43%), Positives = 110/166 (66%), Gaps = 8/166 (4%) Query: 1 MIRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGHLTEAVRRKPYSVILLDEIEKAHPDVFN 60 +I I+MSE+ E H+VS L G+PPGYVGY EGG LTEAVRR PYSV+LLDE+EKAH DV Sbjct: 651 LITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHADVLE 710 Query: 61 VLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNLGSHK-IQAM------EDSDPAVVKMAVM 113 + QV D G + D +GR +DFKNT+I++TSN+ S + +QA E P + + Sbjct: 711 LFFQVFDKGVMDDAEGREIDFKNTIIILTSNVASAQMMQACLNKSQEELPTPEELDTLIR 770 Query: 114 AEVKLHFRPEFINRVDEIVVFHALDERNIGAIAKIQLHILEERLAK 159 ++ F+P F+ R+ ++V ++ + + + I K++L + +R+A+ Sbjct: 771 PQLIKTFKPAFLGRL-KVVPYYPIADVVLVEIIKLKLARIAKRVAE 815 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 676,852 Number of extensions: 25976 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 77 Number of HSP's successfully gapped: 4 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)