BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0001.p (80 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311452|ref|YP_003775544.1| signal transduction containing ... 105 5e-25 >gi|300311452|ref|YP_003775544.1| signal transduction containing EAL and modified HD-GYP domains protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 105 bits (263), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 50/72 (69%), Positives = 61/72 (84%) Query: 9 LFEPLKLPQDIVDALMQRRGRLGRLLDVVERAEYGRIPLRHTDLAAAGVTPEGYCRALIQ 68 + EPLKLP DIV+AL++R GRLG+LLDVVER+E GRIPLRH+DL AAG+ E YCR LIQ Sbjct: 328 IIEPLKLPVDIVNALVRRSGRLGKLLDVVERSEDGRIPLRHSDLDAAGLDAEVYCRCLIQ 387 Query: 69 AYRWASKVSHEA 80 A+ WA++VS EA Sbjct: 388 AFNWATRVSTEA 399 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 220,289 Number of extensions: 6487 Number of successful extensions: 27 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 27 Number of HSP's successfully gapped: 1 Length of query: 80 Length of database: 1,619,870 Length adjustment: 51 Effective length of query: 29 Effective length of database: 1,378,385 Effective search space: 39973165 Effective search space used: 39973165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)