BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0133.0010.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 391 e-110 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 373 e-104 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 355 3e-99 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 391 bits (1005), Expect = e-110, Method: Compositional matrix adjust. Identities = 201/398 (50%), Positives = 254/398 (63%), Gaps = 6/398 (1%) Query: 7 IKEIETSRAEFSHIRRDLHAHPELAFEEVRTADAIAASLKNWGIPVHRGLGGTGLVGIIR 66 IKE+ + IRR LHAHPEL FEE RTA +A L+++G+ V +GG G+V +R Sbjct: 8 IKEL-PKEMDLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLR 66 Query: 67 NGNSTRAIGLRADMDALPIQELNRFEHASQYAGKMHACGHDGHTAMLLAAAWHLNATRNF 126 G STRAI LRADMDALP+ E N F H S AGKMHACGHDGHT MLL AA L+ F Sbjct: 67 KGKSTRAIALRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEF 126 Query: 127 DGTVYLIFQPAEEGKGGAASMITDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASS 186 DGTV+ +FQPAEEG GA MI DGLFE+F ++++ +HNWPG+P GSFG+ G +MASS Sbjct: 127 DGTVHFVFQPAEEGGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASS 186 Query: 187 NEFKITLRGKGSHAALPQHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEA 246 N F TL G+G+H A P SIDPV A A L+ A Q+I+TRN PN AV+S+TQ+ G A Sbjct: 187 NTFSATLFGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTA 246 Query: 247 SNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVVFNRNYPPTINHRA 306 NVIP+ + GTVR+F + LDLIE RM ++E + F + F R YP +NH Sbjct: 247 DNVIPEQATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPE 306 Query: 307 ETAICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNG---DGGHRDHGH 363 T ++ M VGAE VD D E +SEDF+F L KPGCYA +GNG DG + G Sbjct: 307 ATQQAMQAMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADG--KQAGS 364 Query: 364 GLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLALTS 401 LH+ YDFND+++ G YWV L + L + + Sbjct: 365 QATSRELHSPFYDFNDDIIDAGVAYWVSLVRQYLGVAA 402 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 193/389 (49%), Positives = 247/389 (63%), Gaps = 12/389 (3%) Query: 16 EFSHIRRDLHAHPELAFEEVRTADAIAASLKNWGIPVHRGLGGTGLVGIIRNGNSTRAIG 75 E + IRR LH HPEL+FEEV TA +A L+ WG V R +GG GLV +R G+S R+IG Sbjct: 21 EITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSARSIG 80 Query: 76 LRADMDALPIQELNRFEHASQYAGKMHACGHDGHTAMLLAAAWHLNATRNFDGTVYLIFQ 135 LRADMDALPIQE + AS G MHACGHDGHTAMLL AA HL TR FDGT+ LIFQ Sbjct: 81 LRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIFQ 140 Query: 136 PAEEG--KGGAASMITDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASSNEFKITL 193 PAEE GA M+ DGLFE+F E+V+ IHN PG+ G+F SG MA+ + KI + Sbjct: 141 PAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRI 200 Query: 194 RGKGSHAALPQHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEASNVIPDF 253 G+GSHAA P S+DPV A+L ALQT+++RN P D+AV+++ + AG+ASNVIP+F Sbjct: 201 TGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVIPEF 260 Query: 254 GWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVVFNRNYPPTINHRAETAICVE 313 + +VR+F +V +L+E R+R + A +G AE+ + R YP +N AET Sbjct: 261 ATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDFARS 320 Query: 314 VMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGHGLGPCMLHNT 373 V ++VG E V A SEDF++ L +PGC+ +GNG G MLHN Sbjct: 321 VAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQ----------GKPMLHNA 370 Query: 374 SYDFNDNLLPIGATYWVRLTERLLALTSQ 402 YDFND +PIGA YW RL ER + L + Sbjct: 371 GYDFNDANIPIGAAYWTRLVERWMPLQGE 399 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 355 bits (912), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 181/385 (47%), Positives = 231/385 (60%), Gaps = 7/385 (1%) Query: 10 IETSRAEFSHIRRDLHAHPELAFEEVRTADAIAASLKNWGIPVHRGLGGTGLVGIIRNGN 69 + RA ++ +RRDLHAHPEL FEE RTAD + L++ G V RGLGGTG+V + + Sbjct: 11 LHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGAD 70 Query: 70 STRAIGLRADMDALPIQELNRFEHASQYAGKMHACGHDGHTAMLLAAAWHLNATRNFDGT 129 R I LRAD+DALPI E N F HAS G MHACGHDGHT MLL AA L G+ Sbjct: 71 PQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGS 130 Query: 130 VYLIFQPAEEGKGGAASMITDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASSNEF 189 V+ +FQP EEG GA MI DGLFEQF E+V+ +HNWPG+P G FG+ G IMA+ + Sbjct: 131 VHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIMAAGSRL 190 Query: 190 KITLRGKGSHAALPQHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEASNV 249 KIT+ GKG+HAA P +DP+ ++ QTI R+K P D AV+S+ AG+ NV Sbjct: 191 KITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNV 250 Query: 250 IPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVVFNRNYPPTINHRAETA 309 IPD + GT+RT S + ++ +R M LA +G EV F + YP TIN AET Sbjct: 251 IPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATINTPAETD 310 Query: 310 ICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGHGLGPCM 369 C V+R G + + I A ++SEDF FML +PG Y LIGN H Sbjct: 311 FCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHS------- 363 Query: 370 LHNTSYDFNDNLLPIGATYWVRLTE 394 LH+ YDFND+++ G YWV L + Sbjct: 364 LHHPHYDFNDDIIEAGVRYWVALAQ 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,243,808 Number of extensions: 52268 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 3 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)