BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0032.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspi... 202 6e-53 gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspi... 181 7e-47 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 67 3e-12 gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 59 1e-09 gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspi... 57 4e-09 >gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 202 bits (513), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 97/203 (47%), Positives = 130/203 (64%), Gaps = 8/203 (3%) Query: 27 MIADQLVTQGYSVLMLEAGLRIERAQAVENWRNMPFENRVGSDYQGLYPQAPNAPAPL-- 84 +IA+QL+ G V MLEAG R+ RA+ VEN+RN+P + D YP P AP P+ Sbjct: 20 LIAEQLLNAGIPVRMLEAGPRVARAEVVENFRNLPLA--LKGDPSSCYPPRPWAPHPMPS 77 Query: 85 -YFPKNDYIALSGPDGNGFQQGYLRTVGGTTWHWAASCWRHLPVDLKMKSTYGVGRDWPI 143 P + Y+ LSGPD + Q ++R GG+TWHWA +CWR P D+++ S YGVGRDW Sbjct: 78 SENPDDHYLELSGPDK--YAQTFIRYAGGSTWHWAGTCWRLTPEDMRLHSLYGVGRDWAF 135 Query: 144 SYEELEPYYCRAEEEMGVAGPNDPALQSPSERSKPYPMDMVPWGYAEKRFAEVVNPHGYN 203 Y+ LEPYY R E ++G+ GP+DPALQ P +RS PYPM +P+ E+RF EVV GY Sbjct: 136 GYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSPGEQRFTEVVQALGYR 195 Query: 204 SVPIPQGRST-RPWQGRPTCCGN 225 ++P Q R++ P+ RP CC N Sbjct: 196 NIPTAQARNSGMPYDDRPACCAN 218 Score = 176 bits (446), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 97/191 (50%), Positives = 123/191 (64%), Gaps = 2/191 (1%) Query: 229 GASRAEYSSNKMILNNITRVGPATQQALKLGLVGPELDDEIRRRAAFGVDLSISLEPLPE 288 GA R+E++ NN R T ALK GLVG LD+EIRR A D+ ++ E LP+ Sbjct: 354 GAFRSEHAGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPD 413 Query: 289 AHNRLTLSKTRKDPLGLACPDIYYDVGDYVRKGAEAAHKQL-EHIGRLFGAVEFNITTSL 347 A NRLTLS ++KD LGL P I+YDVGDY R+ AE + I GA + ++ Sbjct: 414 AENRLTLS-SKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAMGATDIKVSPKF 472 Query: 348 NANNHVMGGVIMGANPKDSVVDGNCRAHDHANLWLPGGGAMPSASVVNSTLSMAALGLRA 407 + H+MGG IMG +P +SVVD +CRAHDH NL+LPGGGAMPS + NST+SMAALGL+A Sbjct: 473 AQSKHIMGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKA 532 Query: 408 ADDIGKMLAKG 418 AD I + L K Sbjct: 533 ADAILRQLQKA 543 >gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 181 bits (460), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 94/214 (43%), Positives = 134/214 (62%), Gaps = 12/214 (5%) Query: 13 TADVVIVGSGVVGAMIADQLVTQGYSVLMLEAGLRIERAQAVENWRNMPFENRVGSDYQG 72 +ADVV++G+GV GA++A +L G SVL+LE+G R+ R Q VE +R+ F+ SD+ Sbjct: 6 SADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFK----SDFMA 61 Query: 73 LYPQAPNAPAPLYFPK-NDYIALSGPDGNGFQQGYLRTVGGTTWHWAASCWRHLPVDLKM 131 YP +P AP P Y P+ N Y+ GP + F Y+R GGT+WHWAA WR++P D + Sbjct: 62 PYPYSPLAPHPRYTPEPNGYLQQKGP--HAFNAQYIRMFGGTSWHWAAQLWRYVPNDFRQ 119 Query: 132 KSTYGVGRDWPISYEELEPYYCRAEEEMGVAGPNDPALQSPSERSKPYPMDMVPWGYAEK 191 S YGVG+DWPISY++LE YY +AE GV G + +S S R P+PM+ VP + ++ Sbjct: 120 HSLYGVGKDWPISYDDLEDYYYQAEVIAGVGG----SPESGSPRKHPFPMERVPASWLQQ 175 Query: 192 RFAEVVNPHGYNSVPIPQGRSTRPWQGRPTCCGN 225 R + P + + GR++R + GRP CCGN Sbjct: 176 RVTARLAPE-FTVLDDSTGRNSRNYDGRPACCGN 208 Score = 129 bits (323), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 75/202 (37%), Positives = 109/202 (53%), Gaps = 7/202 (3%) Query: 216 WQGR----PTCCGNXPDGASRAEYSSNKMILNNITRVGPATQQALKLGLVGPELDDEIRR 271 W GR P G DG RAE++ ++ ++N ++V T++ + G G L+++I Sbjct: 327 WPGRGPVSPCSIGQFRDGDFRAEHAPFRIDISNASQVAAVTKEVVAEGYFGKRLEEQILF 386 Query: 272 RAAFGVDLSISLEPLPEAHNRLTLSKTRKDPLGLACPDIYYDVGDYVRKGAEAAHKQLEH 331 RAA + + +LE LP+ +NR+TL +KD LGL P +Y++V DYV KG E + Sbjct: 387 RAARRLSIKNALEQLPDRNNRVTLGD-KKDELGLPVPALYWNVDDYVIKGTEQTRACYDA 445 Query: 332 IGRLFGAVEFNITTS--LNANNHVMGGVIMGANPKDSVVDGNCRAHDHANLWLPGGGAMP 389 I GA + S + H+ G + MG +P SV D RAHDH NL++ G G MP Sbjct: 446 IAAKLGATNIKHSKSGAFSNRQHITGTLSMGLDPATSVTDAWGRAHDHENLFMVGTGVMP 505 Query: 390 SASVVNSTLSMAALGLRAADDI 411 + N TL+ AL LR AD I Sbjct: 506 TVGTCNVTLTAMALALRTADKI 527 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 67.0 bits (162), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 60/232 (25%), Positives = 98/232 (42%), Gaps = 32/232 (13%) Query: 15 DVVIVGSGVVGAMIADQLVTQGYSVLMLEAGLRIERAQAVENWRNMPFENRVGS-DYQGL 73 DVVIVG G GA++A ++ G V+ LE G+ + + + + + +YQ Sbjct: 10 DVVIVGFGWTGAIMAKEMTEAGQRVVALERGVY------RDTYPDGAYPKTINELEYQQR 63 Query: 74 YP--QAPNAPAPLYFPKNDYIALSGPDGNGFQQGYLRTVGGTTWHWAASCWRHLPVDLKM 131 + Q N + + K A+ F+ G VGG HW+ WR LP +L+M Sbjct: 64 FKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPG--EGVGGAGLHWSGCHWRILPEELRM 121 Query: 132 KSTYG------------VGRDWPISYEELEPYYCRAEEEMGVAGP---------NDPALQ 170 +S Y +DW +SYEELEPY+ AE+ MG +G ++ Sbjct: 122 RSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGNP 181 Query: 171 SPSERSKPYPMDMVPWGYAEKRFAEVVNPHGYNSVPIPQGRSTRPWQGRPTC 222 ++RS +P+ Y F + G + +P + P+ + C Sbjct: 182 FEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGC 233 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 61/241 (25%), Positives = 89/241 (36%), Gaps = 64/241 (26%) Query: 15 DVVIVGSGVVGAMIADQLVTQGYSVLMLEAGL-----------------RIERAQAVENW 57 DVVIVG G G+++A +L G V LE G R + Sbjct: 10 DVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPEDFAYPKPADQYGYATRNKVFATP 69 Query: 58 RNMPFENRVGSDYQGLYPQAPNAPAPLYFPKNDYIALSGPDGNGFQQGYLRTVGGTTWHW 117 R+ R + Q L + A AP G G VGG+ HW Sbjct: 70 RDAALTVRYNTAQQALPTRKWGAFAP---------------GTG--------VGGSGLHW 106 Query: 118 AASCWRHLPVDLKMKSTYGVG------------RDWPISYEELEPYYCRAEEEMGVAG-- 163 A R P DLK+K+ +D+P ++ E+EP+Y ++ G++G Sbjct: 107 TAVLIRATPTDLKLKTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTT 166 Query: 164 --------PNDPALQSPSERSKPYPMDMVPWGYAEKRFAEVVNPHGYNSVPIPQGRSTRP 215 P + P RS PYP+ + Y FA+V GY+ P P ++ Sbjct: 167 GNLRGQIQPGGDPFEGP--RSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQA 224 Query: 216 W 216 W Sbjct: 225 W 225 >gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 56.6 bits (135), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 58/229 (25%), Positives = 98/229 (42%), Gaps = 37/229 (16%) Query: 14 ADVVIVGSGVVGAMIADQLVTQGYSVLMLEAGLRIERAQAVENWRNMPFENRVG----SD 69 DVV+VG G G+++ +L G SVL LE R + + P+ V S Sbjct: 8 VDVVMVGLGWTGSIMGMELTEAGLSVLALE------RGDMRDTNTDTPYPKAVDELKYSV 61 Query: 70 YQGLYPQAPNAPAPLYFPKNDYIALSGPDGNGFQQGYLRTVGGTTWHWAASCWRHLPVDL 129 L+ + +D +A+ F G VGG +HW +R+LP +L Sbjct: 62 RGALFQDLSRETVTIRHTLSD-VAVPYRQHGSFLLG--DGVGGAGFHWNGMMYRNLPEEL 118 Query: 130 KMKS----TYG--------VGRDWPISYEELEPYYCRAEEEMGVAG----------PNDP 167 +M++ YG +D ++Y+ELEP+Y +E+ M V+G P Sbjct: 119 EMRTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGN 178 Query: 168 ALQSPSERSKPYPMDMVPWGYAEKRFAEVVNPHGYNSVPIPQGRSTRPW 216 L+ ERS +P + + Y+ + + GY+ P P +++P+ Sbjct: 179 PLE--GERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPF 225 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,483,280 Number of extensions: 68226 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 7 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)