BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0031.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 332 5e-92 gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein... 276 4e-75 gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 227 1e-60 gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 219 4e-58 gi|300310653|ref|YP_003774745.1| cytochrome protein [Herbaspiril... 198 7e-52 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 332 bits (850), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 179/366 (48%), Positives = 229/366 (62%), Gaps = 6/366 (1%) Query: 65 SKTAGIGNYSEAQFARALREGVRADGSHLYPAMPYTSYAMLTDSDVHKLYSYFMHAVEPV 124 SKT GIGNY+E QFA ALR GVRADG++LYPAMPYT+YA L+D+DV LY+YFM V PV Sbjct: 98 SKTHGIGNYTEEQFAAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPV 157 Query: 125 DTPTSSQTALPFPFNVRMSMLAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACH 184 D T T LPFP N+R SM AWNA+FL + P +S Q RGRYLAEG AHCS CH Sbjct: 158 DVATRP-TDLPFPMNLRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCH 216 Query: 185 TPRNFLMSEKGGSALAGAPLGSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEGKGQAG 244 TPR +LM EK LAGA +G W+APNITS +G+G WS +LV YL+TG EG+ QA Sbjct: 217 TPRGWLMQEKPDLQLAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAA 276 Query: 245 GAMAEAVTNSFQHLRDDDVAAMVAYLRTVPPVR--DNAQAQPAYSYGKAASDEPALRGLN 302 G+MAEA+T+SF L ++D+ A+ Y++ P + D + + GKA + RG Sbjct: 277 GSMAEAITHSFSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRT 336 Query: 303 GPNEHDSLNSGAVLFSGYCASCHAASGSGSSGQDYPSLFHNTTTGARNPANLVSAILYGV 362 E GA +FS CASCH +G GS YPSLF N+ T N NL++ ILYGV Sbjct: 337 LSAESPEEIRGARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGV 396 Query: 363 ERKIGD-KEILMPRF-DAHSYVNPLTDRQIALIANYVLKQYGNPDVQVSEAYVAQARKGG 420 +R+ + MP F D + +N L++ +A ++NY+LK YG P V V R+GG Sbjct: 397 DRETEQGGHVFMPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGG 456 Query: 421 -EQPVL 425 P+L Sbjct: 457 PRSPLL 462 >gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein [Herbaspirillum seropedicae SmR1] Length = 453 Score = 276 bits (705), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 158/356 (44%), Positives = 204/356 (57%), Gaps = 17/356 (4%) Query: 65 SKTAGIGNYSEAQFARALREGVRADGSHLYPAMPYTSYAMLTDSDVHKLYSYFMHAVEPV 124 SKTAGIG+Y+ QFA ALR+GVRADGS LYPAMPYT+YA L D DV LY+YFM V V Sbjct: 78 SKTAGIGSYTRDQFAAALRQGVRADGSQLYPAMPYTAYARLNDEDVTALYTYFMQGVAAV 137 Query: 125 DTPTSSQTALPFPFNVRMSMLAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACH 184 D + T LPFPFN+R+SM AWN +FLD + F PD ++S Q NRG YL +GLAHCS CH Sbjct: 138 DQ-AAPATDLPFPFNIRLSMKAWNWLFLDKKPFEPDPSQSAQWNRGAYLVQGLAHCSTCH 196 Query: 185 TPRNFLMSEKGGSALAGAPLGSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEGKGQAG 244 TPRNFLM+E+ L G LG+W+AP+IT + W+ L YL +GHA A Sbjct: 197 TPRNFLMAEQLERRLQGGSLGTWYAPDITPAALQAKPAWTREMLADYLASGHAANGATAA 256 Query: 245 GAMAEAVTNSFQHLRDDDVAAMVAYLRTVPPVRDNAQAQPAYSYGKAASDEPALRGLNGP 304 G M EA+ SF LR +D+ AM +YL A A + K + ++G + P Sbjct: 257 GPMLEAIEMSFSRLRREDLEAMASYLLPP------TAATAAPAVNKPPAGLTPVQGDDTP 310 Query: 305 NEHDSLNSGAVLFSGYCASCHAASGSGSSGQDYPSLFHNTTTGARNPANLVSAILYGVER 364 +L+ GA ++ CASCH +G G +G P L + N N+V AIL GV Sbjct: 311 QALAALSPGARVYMNNCASCHNTAGQGLNG--LPPLQSHPVLRQPNGDNVVMAILEGVLP 368 Query: 365 KIGDKEILMPRFDAHSYVNPLTDRQIALIANYVLKQYGNPDVQVSEAYVAQARKGG 420 + G I + + L D Q+A + NY+L+ G V V+ VA R GG Sbjct: 369 EHGQGMI--------GFAHTLNDEQVAAVTNYLLQDIGGSKVSVTTQRVATLRAGG 416 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 227 bits (579), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 141/366 (38%), Positives = 199/366 (54%), Gaps = 31/366 (8%) Query: 66 KTAGIGNYSEAQFARALREGVRADGSHLYPAMPYTSYAMLTDSDVHKLYSYFMHAVEPV- 124 K GIGNYSEAQF+ A+R GVRADG+ LYPAMPY SYA ++D D+H LY+YFM V+PV Sbjct: 78 KEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVA 137 Query: 125 DTPTSSQTALPFPFNVRMSMLAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACH 184 TP +S ++ FPFN+R M WN F + + F S +I RG YL EGL HC +CH Sbjct: 138 STPPAS--SMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKRGAYLVEGLGHCGSCH 195 Query: 185 TPRNFLMSEKGGSA-----LAGAPLGSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEG 239 TPR M+EK + L+G L W P++ G+ WS ++V YL+TG + Sbjct: 196 TPRGVAMNEKASDSSQAQFLSGGDLNGWAVPSL-----RGMPHWSAQDIVDYLQTGRNK- 249 Query: 240 KGQAGGAMAEAVTNSFQHLRDDDVAAMVAYLRTVPPVRDNAQAQPAYSYGKAASDEPALR 299 G M+ V +S HL+ +D+ AM AYL+T+ PV + + G A+ Sbjct: 250 TASVAGEMSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGR-VIPQGVDAT------ 302 Query: 300 GLNGPNEHDSLNSGAVLFSGYCASCHAASGSGSSGQDYPSLFHNTTTGARNPANLVSAIL 359 ++ L G L+ CA+CH +G G+ G +P L T A NP+ L+ IL Sbjct: 303 -VSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGI-FPVLDGATVVNAENPSALLHVIL 360 Query: 360 YGVERKIGDKE---ILMPRFDAHSYVNPLTDRQIALIANYVLKQYGNPDVQVSEAYVAQA 416 G +K ++MP F AH L+D + A +A +V + +GN +VSE V + Sbjct: 361 AGARTPSTEKAPSILVMPGF-AHR----LSDEEAAALATFVRQGWGNHAGKVSEREVGKL 415 Query: 417 RKGGEQ 422 R G ++ Sbjct: 416 RAGMDK 421 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 219 bits (558), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 134/361 (37%), Positives = 197/361 (54%), Gaps = 28/361 (7%) Query: 66 KTAGIGNYSEAQFARALREGVRADGSHLYPAMPYTSYAMLTDSDVHKLYSYFMHAVEPVD 125 K GIGNYSE F RALR G+R DG+ LYPAMPY SYA + +DV LY+YFMH V+ D Sbjct: 83 KENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQ-AD 141 Query: 126 TPTSSQTALPFPFNVRMSMLAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACHT 185 + + +P ++R + W +F V + + RG+YL EGL HC ACHT Sbjct: 142 PAPNRGVDITWPLSMRWPLSIWRKVFAPAVA-VDGPEDNSPLVRGQYLVEGLGHCGACHT 200 Query: 186 PRNFLMSEKGGSA-----LAGAPLGSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEGK 240 PR M EK S L+G + + A N+ D G+G WS+A++V +LKTG Sbjct: 201 PRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDGRDGLGNWSEADIVAFLKTGRNSHS 260 Query: 241 GQAGGAMAEAVTNSFQHLRDDDVAAMVAYLRTVPPVRDNAQAQPAYSYGKAASDEPALRG 300 GG MA+ V NS Q++ ++D++AM YL+++ PV+D PA +Y D+ + Sbjct: 261 AAFGG-MADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGT---PALAY-----DDKTHQA 311 Query: 301 LNGPNEHDSLNSGAVLFSGYCASCHAASGSGSSGQDYPSLFHNTTTGARNPANLVSAILY 360 L ++ + GA+ F CA+CH +SG G + +PSL + T A NPA+L+ +L Sbjct: 312 LRKGSDQ---SPGAMAFLNNCAACHRSSGKGYD-ETFPSLALSPTVNAENPASLIRIVLE 367 Query: 361 GVERKIGDK---EILMPRFDAHSYVNPLTDRQIALIANYVLKQYGNPDVQVSEAYVAQAR 417 G E K + MP F + L+D+++A + ++ +GN VS + VA+ R Sbjct: 368 GAEMPWTHKAPTQFAMPAFGSR-----LSDQEVAEVVTFIRSSWGNQASSVSASDVAKVR 422 Query: 418 K 418 K Sbjct: 423 K 423 Score = 57.0 bits (136), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 54/193 (27%), Positives = 86/193 (44%), Gaps = 40/193 (20%) Query: 161 AAKSQQINRGRYLAEGLAHCSACHTPRNFLMSEKGGSALAG-----APLGSWHAPNITSD 215 +A Q + RG YLA+ C ACHT KGG AG P+G+ ++ NIT D Sbjct: 31 SADQQLVQRGEYLAKA-GDCVACHT-------AKGGKPFAGGLAIATPIGTVYSSNITPD 82 Query: 216 KVSGVGGWSDAELVQYLKTGHAEGKGQAGGAMAEAVTN-SFQHLRDDDVAAMVAYLRTVP 274 K +G+G +S+ + + L+ G + G ++ A+ S+ ++ DV A+ AY Sbjct: 83 KENGIGNYSEEDFDRALR----HGIRKDGASLYPAMPYPSYAKVKPADVKALYAYF---- 134 Query: 275 PVRDNAQAQPAYSYGKAASDEPALR--------------GLNGPNEHDSLNSGAVLFS-- 318 QA PA + G + ++R ++GP ++ L G L Sbjct: 135 --MHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPEDNSPLVRGQYLVEGL 192 Query: 319 GYCASCHAASGSG 331 G+C +CH G G Sbjct: 193 GHCGACHTPRGVG 205 >gi|300310653|ref|YP_003774745.1| cytochrome protein [Herbaspirillum seropedicae SmR1] Length = 974 Score = 198 bits (504), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 133/352 (37%), Positives = 179/352 (50%), Gaps = 21/352 (5%) Query: 69 GIGNYSEAQFARALREGVRADGSHLYPAMPYTSYAMLTDSDVHKLYSYFMHAVEPVDTPT 128 GIGN+S A F RA+REG+ DG HLYPA PYT++A ++++D+ LY+Y M +P T Sbjct: 636 GIGNWSYAAFERAMREGIHRDGRHLYPAFPYTAFAKISEADMQSLYAYLM--AQPAVAST 693 Query: 129 SSQTALPFPFNVRMSMLAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACHTPRN 188 L FPFN+R + WN +F D + F PD A+S NRG YL EG HC+ACH+PRN Sbjct: 694 PPANDLRFPFNLRPLLAGWNTLFHDPQVFQPDPARSDLWNRGAYLVEGAGHCAACHSPRN 753 Query: 189 FLMSEKGGSA-LAGAPLGSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEGKGQAGGAM 247 L +EKGG L G + W AP +T+ S W++ EL YL+TG + G A G M Sbjct: 754 ALGAEKGGRHYLGGGMVDGWEAPALTALSKSPT-PWTEEELFAYLRTGFSARHGIAAGPM 812 Query: 248 AEAVTNSFQHLRDDDVAAMVAYLRTVPPVRDNAQAQPAYSYGKAASDEPALRGLNGPNEH 307 A V Q L DV A+ YL A Q + AA + P E Sbjct: 813 APVVAELAQ-LPPGDVRAIAHYL---------ASFQSGAASSPAAPVPASAAQAPSPAEL 862 Query: 308 DSLNSGAVLFSGYCASCHAASGSGSSGQDYPSLFHNTTTGARNPANLVSAILYGVERKIG 367 S +G LF+G CA+CH + PSL NT + P NLV IL+G+ Sbjct: 863 RSRANGQRLFAGACAACHQEGTGPTLFGVKPSLRVNTNLHSDTPDNLVHVILHGIREPAN 922 Query: 368 DKEILMPRFDAHSYVNPLTDRQIALIANYVLKQY--GNPDVQVSEAYVAQAR 417 MP F N +D Q+ + Y+ ++ G P Q EA +A++R Sbjct: 923 PALGYMPGF-----ANSFSDAQMVDLLAYLRSEFAAGKPAWQGVEAAIARSR 969 Score = 58.9 bits (141), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 61/210 (29%), Positives = 91/210 (43%), Gaps = 27/210 (12%) Query: 145 LAWNAIFLDTRRFVPDAAKSQQINRGRYLAEGLAHCSACHTPRNFLMSEKGGSALAGAPL 204 + W + R PD + I RGR +A C+ CHT + GG AL P Sbjct: 565 MPWRSEIAPIARPDPDLYSAATIERGRRVALA-GDCAVCHTAPGG-ATNAGGHALQ-TPF 621 Query: 205 GSWHAPNITSDKVSGVGGWSDAELVQYLKTGHAEGKGQAGGAMAEAVT-NSFQHLRDDDV 263 G+ ++ NIT D+ +G+G WS A + ++ EG + G + A +F + + D+ Sbjct: 622 GTVYSTNITPDEHTGIGNWSYAAFERAMR----EGIHRDGRHLYPAFPYTAFAKISEADM 677 Query: 264 AAMVAYLRTVPPVRDNAQAQPAYSYGKAASDEPALRGLN-----------GPNEHDSLNS 312 ++ AYL P V A PA + P L G N P D N Sbjct: 678 QSLYAYLMAQPAV---ASTPPANDLRFPFNLRPLLAGWNTLFHDPQVFQPDPARSDLWNR 734 Query: 313 GAVLF--SGYCASCHA---ASGSGSSGQDY 337 GA L +G+CA+CH+ A G+ G+ Y Sbjct: 735 GAYLVEGAGHCAACHSPRNALGAEKGGRHY 764 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,244,415 Number of extensions: 45646 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 12 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)