BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0028.p (660 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 341 1e-94 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 305 8e-84 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 290 3e-79 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 244 2e-65 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 128 1e-30 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 341 bits (874), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 231/573 (40%), Positives = 318/573 (55%), Gaps = 47/573 (8%) Query: 88 ARRRQYLSRSHLQERARSEEHLRLALHAGRL-GSWSLELANSILIVSDACKMHFGIAPES 146 ARRR + + L+E E ++LAL AG + G+W +L + V D FGI E Sbjct: 130 ARRR---TEAALRENV---ERVQLALEAGAIIGTWIWDLPSDRFSVDDGFAAAFGIPAER 183 Query: 147 EF---TYDDLINAIHADDRQRHLDVIEAAMLSKDDFSIEYQVVWPDGSVHWVQIRGQTLH 203 D+L+ ++H DDR+ I A+ ++ +Y+ DG +W++ G+ Sbjct: 184 GLDGIKLDELMLSVHPDDRELLSARIREALARGGAYAHQYRTRRRDGRYYWLEANGRVEM 243 Query: 204 DRLGVPVQMAGVSLDISDRREAEDRLLESQSNLRQFNEMLELRIQERTSELAQANDRLMK 263 G+P + GV +DI RR E + + LR NE LE R++ERT+ L A + Sbjct: 244 TPDGIPSKFPGVLIDIEGRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEE---- 299 Query: 264 EMSERERTQIALVQAQKMEAIGRLTGGIAHDFNNLLNVIIGNVDLIERLSADERI---KR 320 AL QAQKMEA+G+LTGG+AHDFNN+L I G+++L++ A RI +R Sbjct: 300 ----------ALRQAQKMEAVGQLTGGLAHDFNNILAGISGSLELMKVRLAQGRIGDIER 349 Query: 321 MAGSARNATKRGAKLTGQLLAFSRNQTLDLKAVDIGSVIDGMKDLIAVSVGSGIDITISV 380 A++A KR A LT +LLAFSR QTLD K D+ ++ GM +LI SVG I + Sbjct: 350 HLNGAQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIA 409 Query: 381 AEGLPRAVADANQIEMAILNLAINARDAMPDGGNLNIDVQHRYAHD-GVLK----PGAYI 435 A GL D Q+E A+LNL INARDAMP GG L I+ +R+ D G L+ PG Y+ Sbjct: 410 AGGLWHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIGALQRGVPPGQYV 469 Query: 436 VIEVRDTGTGITQEILSKVFDPFFTTKAVGKGTGLGLSQVYGIADQSGGMARIDSTVGKG 495 + V DTG G++ E++++ FDPFFTTK G+GTGLGLS VYG A QSGG RI S +G+G Sbjct: 470 SLSVSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQG 529 Query: 496 TTVEVWLPVAGAMPVREHDQETLDQPLE---PKESGN--ILVIEDDAQVRQFIVECLEIL 550 V ++LP V E + L P E P G+ ILV++D+ VR VE L L Sbjct: 530 AMVCIYLP----RHVGEGGVDDLALPEESHVPAAPGSKTILVVDDEPLVRMVTVEVLRDL 585 Query: 551 GYTVDQAENGWFGLEKLQTAH-FDLLIVDFLMP-GMNGAEVIAAARERDPHLPIIMATGY 608 GY+V +AE+G L ++ DLL+ D +P GMNG ++ A R P LP++ TGY Sbjct: 586 GYSVLEAEDGPSALRVVEACQEIDLLLTDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGY 645 Query: 609 ADMQAVEKVIDLD---AVLRKPFQITELANSVK 638 A+ AV L+ VL KPF LA VK Sbjct: 646 AE-NAVLNHGHLERGMQVLTKPFAADVLARRVK 677 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 305 bits (781), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 209/564 (37%), Positives = 303/564 (53%), Gaps = 50/564 (8%) Query: 96 RSHLQERARSEEHLRLALHAGRLGSWSLELANSILIVSDA-CKMHFGIAPESEFTYDDLI 154 R LQ + SE LR + + L+L L V+ A C+M G + E E L Sbjct: 560 RRGLQALSWSENRLRAIFNQASVCFSELDLQGRYLRVNQALCRM-LGRS-EEEMLGMTLA 617 Query: 155 NAIHADDRQRHLDVIEAAMLSKDDFSIEYQVVWPDGSVHWVQIRGQTLHDRLGVPVQMAG 214 + H DD ++ + + F++E + + PDGS WV + D G P M Sbjct: 618 DVTHPDDVPMNMSLFNRCIEDGRSFTLEKRYLRPDGSTFWVSSDISRIVDGEGRPQAMIA 677 Query: 215 VSLDISDRREAEDRLLESQSNLRQFNEMLELRIQERTSELAQANDRLMKEMSERERTQIA 274 VS DI++RR AE+ LR+ N+ LE R+ E E+ ER R + A Sbjct: 678 VSTDITERRRAEE-------ALRELNDTLEQRVVE--------------EILERSRAEAA 716 Query: 275 LVQAQKMEAIGRLTGGIAHDFNNLLNVIIGNVDLIE---RLSADERIKRMAGSARNATKR 331 L Q QKMEA+G+LTGG+AHDFNN+L +I GN+ L++ RL DER ++ A A R Sbjct: 717 LRQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQSDPRL--DERSRQRVRIAIGAVDR 774 Query: 332 GAKLTGQLLAFSRNQTLDLKAVDIGSVIDGMKDLIAVSVGSGIDITISVAEGLPRAVADA 391 GAKL+ QLLAF+R Q L +++++ V+ M DL+ +VG IDI+ VA L + D Sbjct: 775 GAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGENIDISTRVAHDLWNTMLDP 834 Query: 392 NQIEMAILNLAINARDAMPDGGNLNIDVQHRYAHDGV------LKPGAYIVIEVRDTGTG 445 NQ+E I+N AINARDAMP GG L + + + D V L PG Y+ +++ D G G Sbjct: 835 NQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQISDNGAG 894 Query: 446 ITQEILSKVFDPFFTTKAVGKGTGLGLSQVYGIADQSGGMARIDSTVGKGTTVEVWLPVA 505 ++ E+ + F+PFFTTK G+GTGLGLS YG QS G I S GKGTT+ ++ P Sbjct: 895 MSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIRIYFP-- 952 Query: 506 GAMPVREHDQET-LDQPLEPKESG---NILVIEDDAQVRQFIVECLEILGYTVDQAENGW 561 R D+E +D+ + G ILV+EDD ++ +++ L LGY V +A+NG Sbjct: 953 -----RCLDEEVRIDEVRDLTVHGGDETILVVEDDPNLQLTVIDTLSSLGYRVLKADNGE 1007 Query: 562 FGLEKLQTA-HFDLLIVDFLMPG-MNGAEVIAAARERDPHLPIIMATGYA-DMQAVEKVI 618 LE LQ+ DL+ D +MPG + E+ AR P + I+ +GY D E + Sbjct: 1008 RALEILQSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILFTSGYPRDTIVHEGRL 1067 Query: 619 DLDA-VLRKPFQITELANSVKRAM 641 D +L KP++I +LA +++ + Sbjct: 1068 DAGVELLSKPYRINQLAQRIRQML 1091 Score = 68.9 bits (167), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 53/145 (36%), Positives = 73/145 (50%), Gaps = 16/145 (11%) Query: 503 PVAGAMP-VREHDQETLD---QPL------EPKESGN---ILVIEDDAQVRQFIVECLEI 549 PVA A P VRE QPL EP+E +LV+ED+ +Q + E LE Sbjct: 1126 PVAQADPLVREAHGAGGASAAQPLPDYPRPEPQEDAGGLRLLVVEDNIDSQQLVCELLET 1185 Query: 550 LGYTVDQAENGWFGLEKLQTAHFDLLIVDFLMPGMNGAEVIAAARERDPHLPIIMATGYA 609 LG+ +G L+ L+ FD+L D +PGM+G E+ AA R P L +I A+GY Sbjct: 1186 LGHQARGVISGEEALQALEEQGFDVLFTDVNLPGMSGVELAKAALGRFPDLAVIFASGYG 1245 Query: 610 DMQAVEKVIDLDA-VLRKPFQITEL 633 M V + + A L KP+ I +L Sbjct: 1246 TM--VARNVGFAAHALPKPYDIDQL 1268 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 42/155 (27%), Positives = 75/155 (48%), Gaps = 7/155 (4%) Query: 93 YLSRSHLQERARSEEHLRLALHAGRLGSWSLELANSILIVSDACKMHFGIAPESEFTYDD 152 ++ + + ER +EE LR+ AG +G + + L VSD + +G+ PE E T Sbjct: 292 FIEGNDVTERMLAEERLRIVDRAGEIGIFEWFPDSGRLSVSDRFRRLWGLPPEGEVTAAQ 351 Query: 153 LINAIHADDRQRHLDVIEAAML---SKDDFS-IEYQVVWPDGSVHWVQIRGQTLHDRLGV 208 L+ I D Q H ++ A + S++ EY + PDG W+ +G+ + D Sbjct: 352 LVGMI---DEQFHPNLGTARIKEGGSRNPLEYTEYHITRPDGQQRWLARKGEVVQDDPRT 408 Query: 209 PVQMAGVSLDISDRREAEDRLLESQSNLRQFNEML 243 + GV+ D++ R+ E L S++++R N + Sbjct: 409 GSRYVGVAFDVTARKLMEQALAASEASVRANNAFV 443 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 290 bits (741), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 182/432 (42%), Positives = 252/432 (58%), Gaps = 45/432 (10%) Query: 229 LLESQSNLRQFNEMLELRIQERTSELAQANDRLMKEMSERERTQIALVQAQKMEAIGRLT 288 L ES++ LR NE LE + RT EL T+ AL +QK+EAIG+LT Sbjct: 427 LAESEARLRAINESLEAEVSARTRELMA--------------TEEALRHSQKIEAIGQLT 472 Query: 289 GGIAHDFNNLLNVIIGNVDLIE-RL--SADERIKRMAGSARNATKRGAKLTGQLLAFSRN 345 GG+AHDFNNLL I G++DL+ RL E + R A +T+R A LT +LLAF+R Sbjct: 473 GGLAHDFNNLLGGISGSLDLMRVRLVQGRGEELLRYVELAARSTQRAASLTQRLLAFARR 532 Query: 346 QTLDLKAVDIGSVIDGMKDLIAVSVGSGIDITISVAEGLPRAVADANQIEMAILNLAINA 405 QTL K + ++I GM++LI ++G I + + A GL D Q+E A+LNL INA Sbjct: 533 QTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWPTFVDGPQLESALLNLCINA 592 Query: 406 RDAMPDGGNLNIDVQH-----RYAHDGVLKPGAYIVIEVRDTGTGITQEILSKVFDPFFT 460 RDAMP GG L I+ + R+AH+ L PG Y+ I V D+G G+ ++L++ FDPFFT Sbjct: 593 RDAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMPPDVLARAFDPFFT 652 Query: 461 TKAVGKGTGLGLSQVYGIADQSGGMARIDSTVGKGTTVEVWLPVAGAMPVREHDQETLDQ 520 TK +G GTGLGLS VYG A QSGG R+ S VG+GTTV ++L P +HD + D+ Sbjct: 653 TKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYL------PRHQHDGDEQDE 706 Query: 521 --PLEPKESGN-----ILVIEDDAQVRQFIVECLEILGYTVDQAENGWFGLEKLQTAH-F 572 L+ +G+ +LV++D+A +R + E L GY V QAE+G L LQ+ Sbjct: 707 ADALDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAEDGPGALRILQSKQRI 766 Query: 573 DLLIVDFLMP-GMNGAEVIAAARERDPHLPIIMATGYADMQA-----VEKVIDLDAVLRK 626 DLLI D +P G+NG +V A R P L ++ TGYA+ A +E+ + L + K Sbjct: 767 DLLITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNGHLERGMQL---MTK 823 Query: 627 PFQITELANSVK 638 PF++ LA V+ Sbjct: 824 PFKMEALARRVQ 835 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 244 bits (622), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 167/440 (37%), Positives = 239/440 (54%), Gaps = 28/440 (6%) Query: 219 ISDRREAEDRLLESQSNLRQF-NEMLELRIQERTSELAQANDRLMKEMSERERTQIALVQ 277 +++RRE LL + N F N + + ++ L + ++S R + AL Q Sbjct: 111 VAERREIATELLNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQL-DISRRRDAEQALGQ 169 Query: 278 AQKMEAIGRLTGGIAHDFNNLLNVIIGNVDLIE-RLSADE----RIKRMAGSARNATKRG 332 A+KMEA+G+LTGGIAHDFNNLL VI G +D+I L +D+ R+ R A S R A+ + Sbjct: 170 ARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKA 229 Query: 333 AKLTGQLLAFSRNQTLDLKAVDIGSVIDGMKDLIAVSVGSGIDITISVAEGLPRAVADAN 392 A LT QLLAF+R Q L+ + +++ S+ +G DL++ ++G I + +A GL D Sbjct: 230 AMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPT 289 Query: 393 QIEMAILNLAINARDAMPDGGNLNIDVQHRYAHDG------VLKPGAYIVIEVRDTGTGI 446 Q+E+A+LN+ INARDAM G + I + D LK G Y+ I V D G GI Sbjct: 290 QMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGI 349 Query: 447 TQEILSKVFDPFFTTKAVGKGTGLGLSQVYGIADQSGGMARIDSTVGKGTTVEVWLP--- 503 +IL +V DPFFTTK GKGTGLGLS VYG QSGG RI S GTTV ++ P Sbjct: 350 APDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQ 409 Query: 504 --VAGAMPVREHDQETLDQPLEPKESGNILVIEDDAQVRQFIVECLEILGYTVDQAENGW 561 V G E+D + Q + +ILV++D +V + LE LGY V + Sbjct: 410 DQVGGGF---EYDNRSQSQGGQE----HILVVDDRLEVAELAQTMLESLGYRVSMVNSPA 462 Query: 562 FGLEKLQTAH-FDLLIVDFLMPG-MNGAEVIAAARERDPHLPIIMATGYADMQAVEKVID 619 L+ +++ DLL D +MPG MNG + A+ P + +++ TGYA D Sbjct: 463 EALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGAD 522 Query: 620 LD-AVLRKPFQITELANSVK 638 + VL KP++ ELA ++ Sbjct: 523 GEFPVLDKPYRHDELARKIR 542 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 128 bits (322), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 105/389 (26%), Positives = 173/389 (44%), Gaps = 74/389 (19%) Query: 154 INAIHADDRQRHLDVIEAAMLSKDDFSI-EYQVVWPDGSVHWVQIRGQTLHDRLGVPVQM 212 N IH DD + + AML +D I EY+ +GS W+ + + D G P ++ Sbjct: 83 FNHIHPDDTAQIFSSL--AMLFVEDQKIYEYRFRTSNGSYLWMHDTLRLIRDPDGTPQEV 140 Query: 213 AGVSLDISDRREAEDRLLESQSNLRQFNEMLELRIQERTSELAQANDRLMKEMSERERTQ 272 G+ DI+ R+E ED L R+ E L++ Q Sbjct: 141 IGLMTDITGRKEMEDTLQRQAEEQRKLIEQLKM-------------------------AQ 175 Query: 273 IALVQAQKMEAIGRLTGGIAHDFNNLLNVIIGNVD--------LIERLSADERIKRMAGS 324 L+Q++K+ +IG+L G+AH+ NN + + N++ L+E + + ++ A Sbjct: 176 AQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALP 235 Query: 325 ARNATKRGAKLTGQL-LAFSRNQTLDLKAVD------IGSVIDGMKDLIAVSVGS----- 372 A A ++ + LAF +L A + +++ +KD + + Sbjct: 236 AAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTD 295 Query: 373 ---GIDITISVAEG--------------LPRAVADANQIEMAILNLAINARDAMPDGGNL 415 G+D T+++A LP A+++ +NL +NA A+ + G + Sbjct: 296 LHRGLDSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTV 355 Query: 416 NIDVQHRYAHDGVLKPGAYIVIEVRDTGTGITQEILSKVFDPFFTTKAVGKGTGLGLSQV 475 I + G + + + DTG GI E L+++F+PFFTTK VG GTGLGLS Sbjct: 356 TI---------CTARAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLS 406 Query: 476 YGIADQSGGMARIDSTVGKGTTVEVWLPV 504 YGI + GG R+ S G GTT + LP+ Sbjct: 407 YGIVKKHGGDIRVSSAPGCGTTFTIELPI 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,053,290 Number of extensions: 89268 Number of successful extensions: 551 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 524 Number of HSP's successfully gapped: 38 Length of query: 660 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 569 Effective length of database: 1,188,985 Effective search space: 676532465 Effective search space used: 676532465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)