BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0026.p (481 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311774|ref|YP_003775866.1| two component sensor histidine ... 597 e-172 gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine ... 124 2e-29 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 66 6e-12 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 65 1e-11 gi|300311272|ref|YP_003775364.1| two component sensor histidine ... 60 5e-10 >gi|300311774|ref|YP_003775866.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 460 Score = 597 bits (1540), Expect = e-172, Method: Compositional matrix adjust. Identities = 298/425 (70%), Positives = 343/425 (80%), Gaps = 4/425 (0%) Query: 45 AGHKNMLQLIQLRWIAVFGQITTIIVVIFGFDIQLPLPHMLEVLACLIAFNIGSHLRWHE 104 AGH+NML LIQLRW+AV GQ++TI V+ FD+ LPLPHMLEVL+CL+AFNI SHLRWHE Sbjct: 28 AGHQNMLLLIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCLVAFNIASHLRWHE 87 Query: 105 RPFVTNRELFLALLVDVATLTAQLYLSGGASNPFAFLYLLQVILSAVLLETWSTWTIVCI 164 R TN ELFLALLVDVA LT QLYLSGG +NPFAFLYL+Q+ILSAVLL+ WS+W IV + Sbjct: 88 RRIATNAELFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIVGV 147 Query: 165 TGLCFAGLSQFFMPLSLPPDHDEGLFSLYVEGMLISFLLIASLLAIFITRISSNRRAGDA 224 T LC AGL+ F PL+LPPDH+ GL SLYVEG LI FLL A L+ FITRISSN RAGDA Sbjct: 148 TMLCLAGLALFSEPLALPPDHNAGLLSLYVEGTLICFLLNAGLMVFFITRISSNLRAGDA 207 Query: 225 QLAALRQRAAEEDHIVRMGLLASGAAHELGTPLATLAVILGDWRRMPEFSSNPVLIEEIS 284 LA LRQRAAEEDHIVRMGLLASGAAHELGTPLAT++VILGDW+RMPE S ++++I+ Sbjct: 208 LLADLRQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEISQRQDMLDDIA 267 Query: 285 EMQIQLQRCKTIVSGVLLSAGEARGESSVKTTLNTFLGDIAEEFRTTRPIVAFDYRNLIE 344 EM+ QLQRCK+IVSGVLLSAG+ARGESS+KTTLNTFL +AE++R +RP+ FDY+NLI Sbjct: 268 EMEAQLQRCKSIVSGVLLSAGQARGESSIKTTLNTFLDSVAEDWRKSRPVAVFDYQNLIG 327 Query: 345 HDMPVAFDSALKQMICNVLDNALEASPRWLSLTAAREADELILSVDDTGPGFVPSMLDQI 404 D+PVAFDSA+KQM+ NVLDNA E SP WLSL RE L++ V D GPGF M++QI Sbjct: 328 QDLPVAFDSAIKQMLGNVLDNAFEVSPHWLSLEVRREGGLLLMEVSDQGPGFPEWMINQI 387 Query: 405 GKPYQSTKAKTGGGLGLFFVVNVARKLGGAVTAHNRVENGHVAGATVRLTLPLSAISLES 464 GKPYQSTK K G GLGLFFVVNVARKLGG V A N AGA V L LPLSAISLE+ Sbjct: 388 GKPYQSTKGKPGRGLGLFFVVNVARKLGGRVWASNLPG----AGARVTLELPLSAISLEA 443 Query: 465 ENTAV 469 + V Sbjct: 444 DAEPV 448 >gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 124 bits (310), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 138/436 (31%), Positives = 212/436 (48%), Gaps = 39/436 (8%) Query: 47 HKNMLQLIQLRWIAVFGQITTIIVVIFGFDIQLPLPHMLEVLACLIAFNIGS--HLRWHE 104 H + +L LRW V G++ + I LP+P +L ++ N + LRW Sbjct: 18 HVLLSRLFWLRWAMVAGELLVLAGAHGWLHIALPVPQLLALIGLQALLNGATWLRLRW-T 76 Query: 105 RPFVTNRELFLALLVDVATLTAQLYLSGGASNPFAFLYLLQVILSAVLLETWSTWTIVCI 164 RP RELFL LL+D+A LTA LY SGG++NPF YL + A L + Sbjct: 77 RP-AEQRELFLQLLLDLAVLTALLYFSGGSTNPFVSFYLPALAAGAASLAWPYALLLALC 135 Query: 165 TGLCFAGLSQFFMPLSLPPDHDEG-LFSLYVEGMLISFLLIASLLAIFITRISSNRRAGD 223 ++GL + PL + HD+G + ++ GM I+F + A L+ F++RI++ R+ + Sbjct: 136 ALGAYSGLVYVYEPLHI---HDQGQAMAYHLAGMWINFSISALLITWFVSRIAAAVRSRE 192 Query: 224 AQLAALRQRAAEEDHIVRMGLLASGAAHELGTPLATLAVILGDWRRMPEFSSNPVLI--- 280 +LA R+R + I+ +G A+GAAH L TPL+T+AVI G+ RR + ++ P L Sbjct: 193 TELARARERQLQSQRILALGTQAAGAAHALNTPLSTVAVIAGELRR--DMATQPALAAYD 250 Query: 281 EEISEMQIQLQRCKTIVSGVLLSA-GEARGESSVKTTLNTFLGDIAEEFRTTRPIVAFDY 339 EE+ ++ Q+ CK + + A + R E S T +L + E +R P V Sbjct: 251 EELRVVEEQIAVCKAALESMRQDADAQTRNEDSAPIT--PWLTTLLEGWRLRHPAVLLSA 308 Query: 340 RNLIEHDMPVAFDSALKQMICNVLDNALEA-----SPRWLSLTAAREADE---------L 385 + + A L Q++ +LDNA A S +S+ R ADE Sbjct: 309 -EVHQPGWVAARLEDLSQILLTLLDNAAHAVRERGSEGRVSIR-MRAADEPAGRPAQKAA 366 Query: 386 ILSVDDTGPGFVPSMLDQIGK-PYQSTKAKTGGGLGLFFVVNVARKLGGAVTAHNRVENG 444 I+ + D G G P +L ++G+ P S+ +G GL L F A LGG + R+ + Sbjct: 367 IIEIADNGSGIAPELLQRLGRAPVASSSGGSGIGLMLAFA--SAAHLGGKL----RLYST 420 Query: 445 HVAGATVRLTLPLSAI 460 G L LPLSA+ Sbjct: 421 PGQGTCAELRLPLSAM 436 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 66.2 bits (160), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 64/215 (29%), Positives = 101/215 (46%), Gaps = 12/215 (5%) Query: 249 AAHELGTPLATLAVILGDWRRMPEFSSNPVLIEEISEMQIQLQRCKTIVSGVLL--SAGE 306 AAHEL TPL L++ R P + E ++ ++ LQR + +V+ +L A Sbjct: 235 AAHELRTPLTALSLQAERLGRAP---MSETARERLTRLETGLQRARQLVTQLLALARAQT 291 Query: 307 ARGESSVKTTLNTFLGDIAEEFRTTRPIVAFDYRNLIEHDMPVAFD-SALKQMICNVLDN 365 G + +L+ L ++ EE D E D V L Q++ N++DN Sbjct: 292 PAGAALQPCSLHQVLREVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNLIDN 351 Query: 366 ALEASPRW--LSLTAAREADELILSVDDTGPGFVPSMLDQIGKP-YQSTKAKTGGGLGLF 422 A+ +P +SL R A + L + DTGPG S +++ +P Y+ + G GLGL Sbjct: 352 AIAYTPAGGAVSLRMERHAQWVALEICDTGPGIAASERERVFQPFYRIDPSGQGSGLGLA 411 Query: 423 FVVNVARKLGGAVTAH-NRVENGHVAGATVRLTLP 456 V ++AR+LG VT + +G VRL +P Sbjct: 412 IVQSIARRLG--VTLRLEAADPAQQSGLRVRLLIP 444 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 75/302 (24%), Positives = 132/302 (43%), Gaps = 53/302 (17%) Query: 212 ITRISSNRRAGDAQLAALRQRAAEEDHIVRMGLLASGAAHELGTPLATLAV---ILGDW- 267 + R + +R QL + + + + + +G LA+G AHE+ P+ ++ LG++ Sbjct: 157 LQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYV 216 Query: 268 ---------------------------RRMPEFSSNPVLIEEISEMQIQ----LQRCKTI 296 R + L E+++E+ + LQR + I Sbjct: 217 ATLLEGIQLHQCLQGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNI 276 Query: 297 VSGVLLSAGEARGESSVKTT-----LNTFLGDIAEEFRTTRPIVAFDYRNLIEHDMPVAF 351 V L GES+ + T L++ L E + IV + E + Sbjct: 277 VQS--LKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIV----KQYGELPLVKCL 330 Query: 352 DSALKQMICNVLDNALEA-SPRW-LSLTAAREADELILSVDDTGPGFVPSMLDQIGKPYQ 409 S L Q+ N+L NA +A S R +++ AR D++ +S+ DTG G P L++I +P+ Sbjct: 331 ASELNQVFMNLLVNAAQAISERGTVTICTARAGDKVSVSISDTGHGIAPENLNRIFEPFF 390 Query: 410 STK-AKTGGGLGLFFVVNVARKLGGAVTAHNRVENGHVAGATVRLTLPLSAISLESENTA 468 +TK +G GLGL + +K GG + RV + G T + LP+ S+ ++++A Sbjct: 391 TTKPVGSGTGLGLSLSYGIVKKHGGDI----RVSSAPGCGTTFTIELPIDPASVLAQSSA 446 Query: 469 VE 470 E Sbjct: 447 TE 448 >gi|300311272|ref|YP_003775364.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 69/223 (30%), Positives = 104/223 (46%), Gaps = 29/223 (13%) Query: 250 AHELGTPL-ATLAVILGDWRRMPEFSSNPVLIEEISEMQIQLQRCKTIVSGV----LLSA 304 AHEL TPL A A + G + F +P +++S + QL + ++S + L+SA Sbjct: 183 AHELRTPLTAAKARLQGIMDGI--FDRSP---QQLSMIMRQLDQLNRLISDLYLLSLVSA 237 Query: 305 GE---ARGESSVKTTLNTFLGDIAEEFRTTRPIVAFDYRNLIEHDMPVAFD-SALKQMIC 360 G+ R + ++ L L A R P+ Y EH +P+ D L Q I Sbjct: 238 GQLALVRSQFQLRPLLEERLAWAAPSLRLL-PMSTALY--CCEH-LPLNADRDRLGQAIS 293 Query: 361 NVLDNALE--ASPRWLSLTAAREADELILSVDDTGPGFVPSML----DQIGKPYQSTKAK 414 ++DNAL AS + L+++A ++ + V+D GPGF P L D+ + S Sbjct: 294 ILIDNALRYAASGKLLTISAGLHDGQIHIDVEDDGPGFAPEHLELACDRFWRAEHSRSRH 353 Query: 415 TGG-GLGLFFVVNVARKLGGAVTAHNRVENGHVAGATVRLTLP 456 GG GLGL V + GG ++ HNR GA + L LP Sbjct: 354 AGGSGLGLAVAVAICEAHGGKLSVHNRP----AGGARMHLELP 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,308,541 Number of extensions: 47160 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 5 Length of query: 481 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 392 Effective length of database: 1,198,455 Effective search space: 469794360 Effective search space used: 469794360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)