BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0017.p (346 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 525 e-150 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 116 3e-27 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 94 2e-20 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 92 5e-20 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 89 6e-19 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 525 bits (1351), Expect = e-150, Method: Compositional matrix adjust. Identities = 268/345 (77%), Positives = 313/345 (90%) Query: 2 QKKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLP 61 QK GREVKLTLALF+LG STAGIA LPT+EH+G AI+LLS+LR+GQGLALGG+WDGLP Sbjct: 101 QKHYGREVKLTLALFMLGFSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLP 160 Query: 62 SLLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFA 121 SLLA+N P++KRGWYA LGQLGAP+GF+VA +FAY++ +L++ DFLEWGWRYPFYVAFA Sbjct: 161 SLLAMNAPQHKRGWYAALGQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFA 220 Query: 122 INVVALFARLRLVASEEYASLLAERELEPTSVVEIARTQGRNLIIGALAALASYALFHLV 181 INVVALF+RLRLV++ EYA LL ERELEP VVE+A TQGRN+IIGALAALASYALFHLV Sbjct: 221 INVVALFSRLRLVSAPEYAKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLV 280 Query: 182 SVFPLSWISLYSMRSMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTLGALAVLIAVL 241 +VFPLSWI LYS+R +DFL+VQ++G ++M VGIV+SG IADRFGRR TLG++A+LIA+L Sbjct: 281 TVFPLSWIKLYSLREATDFLVVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAIL 340 Query: 242 SGFVPTLINGGDTGQDIFILVGFALLGLSYGQAAGAVTANFPSKYRYTGAALTSDLAWLV 301 S FVPTL+NGG+ GQ+ FIL+GFALLGLSYGQAAGAVTANF ++YRYTGAALTSDLAWLV Sbjct: 341 SAFVPTLMNGGEAGQNTFILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLV 400 Query: 302 GAGFAPLVALGLSAHFGLAYVSIYLLSGAVCSLAALSVNRALDIR 346 GAGFAPLVALGLSAHFG+AYVS YLLSGAV SLAALS+NRAL+ R Sbjct: 401 GAGFAPLVALGLSAHFGMAYVSFYLLSGAVGSLAALSLNRALEAR 445 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 85/334 (25%), Positives = 159/334 (47%), Gaps = 6/334 (1%) Query: 3 KKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPS 62 + GR+ + + + L+ L+ A IAF PTY +G A +++ V R+ QG A GG + + Sbjct: 75 DRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTA 134 Query: 63 LLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAI 122 L + P +KRG Y +G + + A + NLS + WGWR PF + I Sbjct: 135 FLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLI 194 Query: 123 NVVALFARLRLVASEEYASLLAERELEPTSVVEIARTQGRNLIIGALAALASYALFHLVS 182 V L+ R + ++++ + P ++++ +T R +++ + F++V Sbjct: 195 GPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAFYVVL 254 Query: 183 VFPLSWISLYSMRSMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTL--GALAVLIAV 240 V ++ + + MVQ+ L+ I +G ++DR GRRT L +LA L+ V Sbjct: 255 VNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLYSSLAFLLMV 314 Query: 241 LSGFVPTLINGGDTGQDIFIL--VGFALLGLSYGQAAGAVTANFPSKYRYTGAALTSDLA 298 FV ++ + + ++ + ++G ++G A++ FP++ R TG A+ +LA Sbjct: 315 YPLFV--WVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAYNLA 372 Query: 299 WLVGAGFAPLVALGLSAHFGLAYVSIYLLSGAVC 332 ++ GFAP + L+ G + + A C Sbjct: 373 AMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAAC 406 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 62/233 (26%), Positives = 114/233 (48%), Gaps = 12/233 (5%) Query: 6 GREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPSLLA 65 GR+ L +FL+G ST G+ LPTY+ +G A LL ++R+ QG A+ G G S++ Sbjct: 85 GRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFAVAGEISGASSMIL 144 Query: 66 LNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAINVV 125 + P +RG+++ G G I+A VF L + ++ F WGWR PF ++F + + Sbjct: 145 EHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGWRIPFLLSFLVIIA 204 Query: 126 ALFARLRLVASEEYASLLAERELEPTSVVEIARTQGRNLIIGALAALASYALFHLVSVFP 185 R + + + + E + VV+ R N++ + AL +++ V Sbjct: 205 GWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNML-----RVVCMALMNVIPVVA 259 Query: 186 LSWISLYSMR-------SMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTL 231 + + Y+++ + +L + V G ++ ++ I G ++DR GRR + Sbjct: 260 TIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRRPPI 312 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 92.4 bits (228), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 81/307 (26%), Positives = 141/307 (45%), Gaps = 7/307 (2%) Query: 3 KKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPS 62 ++GR+ L++ + L+ + T I PTYE +G A +++ V R+ QG + GG + Sbjct: 87 DRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATA 146 Query: 63 LLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAI 122 L P+ +R +Y+ Q V ++ V ++ +L + WGWR PF + I Sbjct: 147 FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIII 206 Query: 123 NVVALFARLRLVASEEYASLLAERELEPTSVVEIARTQGRNLIIG-ALAALASYALFHLV 181 V + R L EE + L E++ + + E+ R R ++ L + + L+ Sbjct: 207 GPVGYYIRHHL---EETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVILWTVCTYVLL 263 Query: 182 SVFPLSWISLYSMRSMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTLGALAVLIAVL 241 P + + + + F+ V G +M++ ++ G +ADR GRR L A LI +L Sbjct: 264 FYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIV-GRLADRIGRRPLLSGSAALILML 322 Query: 242 SGFVPTLINGGDTGQD--IFILVGFALLGLSYGQAAGAVTANFPSKYRYTGAALTSDLAW 299 + + IN + IF LV L+ G A + FP+K TG ++ +LA Sbjct: 323 AYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAYNLAV 382 Query: 300 LVGAGFA 306 + GFA Sbjct: 383 TIFGGFA 389 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 94/341 (27%), Positives = 155/341 (45%), Gaps = 13/341 (3%) Query: 3 KKLGREVKLTLALFLLGLSTAGIAFLPTYEHLGFTAIVLLSVLRIGQGLALGGSWDGLPS 62 ++GR L + L L+ L T IAF+P+Y +G A +L+ + R+ QG + G G+ Sbjct: 78 DRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSV 137 Query: 63 LLALNVPKNKRGWYAMLGQLGAPVGFIVAGLVFAYLIGNLSSDDFLEWGWRYPFYVAFAI 122 LA +G+Y V IVA + L +L+ + WGWR PF++ I Sbjct: 138 YLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLI 197 Query: 123 NVVALFARLRLVASEEYASLLAERELEPTSVVEIARTQGRN---LIIGALAALASYALFH 179 V R L +EE+ R+ P S +I R+ N +I G + + F+ Sbjct: 198 VPVLFVIRRSLQETEEF----MRRKHRP-STGQIFRSMIENWKLVIAGMMLVSMTTVSFY 252 Query: 180 LVSVFPLSWISLYSMRSMSDFLMVQVFGAVLMMVGIVLSGPIADRFGRRTTLGALAVLIA 239 L++V+ ++ S L+V + + + + + G ++DR GRR L VL Sbjct: 253 LITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVL-T 311 Query: 240 VLSGF--VPTLINGGDTGQDIFILVGFALLGLSY-GQAAGAVTANFPSKYRYTGAALTSD 296 +L+ + V L++ + + + + + L SY G A+T P+ R G +L Sbjct: 312 ILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYS 371 Query: 297 LAWLVGAGFAPLVALGLSAH-FGLAYVSIYLLSGAVCSLAA 336 LA V GF P +A GL + A I++ + AVC L A Sbjct: 372 LATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIA 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,090,983 Number of extensions: 49546 Number of successful extensions: 317 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 11 Length of query: 346 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 260 Effective length of database: 1,212,660 Effective search space: 315291600 Effective search space used: 315291600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)