BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0016.p (370 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 313 2e-86 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 234 1e-62 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 72 5e-14 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 313 bits (801), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 166/350 (47%), Positives = 223/350 (63%), Gaps = 5/350 (1%) Query: 1 LSALSQSTAHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNL 60 L AL+ +T+HIGL AT STTY++PY +AR+FA+LDHLS GRA WNVVTS ++ EA NF L Sbjct: 86 LGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGL 145 Query: 61 DKHPEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDSSKLHAIHHKGKHFSVR 120 + H RY+RA+EF++V LW SW+ DA R Q + D+ K+ AI H+G HFSVR Sbjct: 146 KETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQDR--YADADKVAAIDHQGAHFSVR 203 Query: 121 GPLNVARPVQGHPVVVQAGSSGPGQELAARTAEVIFTAQQSLEDAQHFYSGLKSRLAKYG 180 GPLN+ QGHPV++QAGSS GQ+ AAR AEV+F Q L Q FY LK+R ++G Sbjct: 204 GPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFG 263 Query: 181 REPDELKIMPGVFPVVGRSESEAREKFEELQSLIHPSVGLSLLQQHLGGIDLSGYPLDGP 240 R P +++I+ V P VG+S ARE E+ +L P VGLS L H+ +D SG+ LD P Sbjct: 264 RRPQDIRILSAVMPFVGQSRQHARELQEQANALADPLVGLSTLSSHM-NVDFSGFALDAP 322 Query: 241 LPENLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPESIVDRLEEWF 300 L + + +G + F+L+ A++ QLT+ A I GT + D L+ Sbjct: 323 LGD--VQVSGIQGLFKLLRAISAERQLTLADAGRLYAQGVLTPQIAGTAADVADWLQSII 380 Query: 301 LKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRTEYEGKTLRENLG 350 + GADGF I P LP G DDFV+ V+PEL+RRG FR +Y G+ LR++L Sbjct: 381 VANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDYAGRQLRQHLA 430 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 234 bits (596), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 134/350 (38%), Positives = 195/350 (55%), Gaps = 10/350 (2%) Query: 3 ALSQSTAHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDK 62 A++ T H+G T + TY P+ AR+ ++LDHLS GR GWN+VT + +S A L + Sbjct: 92 AMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHE 151 Query: 63 HPEHGARYERAEEFVDVVRGLWD-SWEDDAFRFDQAEGVHFDSSKLHAIHHKGKHFSVRG 121 EH RY+RA+EF++VV LW+ SWED A D+A V+ D +K+H I H G ++ V G Sbjct: 152 QLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEG 211 Query: 122 PLNVARPV-QGHPVVVQAGSSGPGQELAARTAEVIFTAQQSLEDAQHFYSGLKSRLAKYG 180 ++++ P Q PV+ QAGSSG GQ AR AE +F + QS + + ++ + G Sbjct: 212 -IHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREG 270 Query: 181 REPDELKIMPGVFPVVGRSESEAREKFEELQSLIHPSVGLSLLQQHLGGIDLSGYPLDGP 240 R+PD +KI G+ V GR+E +A+E++ E P GL+ GID S Y LD P Sbjct: 271 RDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAHYSAGT-GIDFSQYELDEP 329 Query: 241 LPENLQEPNGTKSRFQLVTALARRE-QLTIRQLYLRVATARGHWSIHGTPESIVDRLEEW 299 + P T + L + + E T R+L ++ + SI G P+ + D LE W Sbjct: 330 I-----RPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVGAPDRVADELESW 384 Query: 300 FLKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRTEYEGKTLRENL 349 G DG+N+ P +DFVELV+P L+ RG ++ EY TLRE L Sbjct: 385 VADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGTLREKL 434 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 60/215 (27%), Positives = 98/215 (45%), Gaps = 38/215 (17%) Query: 3 ALSQSTAHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDK 62 AL +T + ++A + P VA++ A++DH+SNGR NVV+ W + E F Sbjct: 85 ALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE---FQAIG 141 Query: 63 HP--EHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDSSKLHAIHHKGKHFSVR 120 P EH RY R+ EF+ ++G+W D F F KG + Sbjct: 142 EPWLEHDERYRRSNEFIRALKGIWTQ---DDFTF------------------KGDFYRFN 180 Query: 121 GPLNVARPVQG-HPVVVQAGSSGPGQELAARTAEVIFTAQQSLEDAQHFYSGLKSRL--- 176 +PVQ HP + Q GSS ++ AA ++ FT +LE GLK+++ Sbjct: 181 NYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLE-------GLKAQIDDI 233 Query: 177 -AKYGREPDELKIMPGVFPVVGRSESEAREKFEEL 210 AK + +++I F + +E EA+ +++ Sbjct: 234 QAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDI 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,287,726 Number of extensions: 56694 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 4 Length of query: 370 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 283 Effective length of database: 1,207,925 Effective search space: 341842775 Effective search space used: 341842775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)