BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0010.p (256 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 87 2e-18 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 67 2e-12 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 59 3e-10 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 46/120 (38%), Positives = 71/120 (59%) Query: 1 VLPSPQSVLLTFIDLAASGEMFDNLKISLLRVLLGFGVGLAGGLLLGVAMGLSRVFKDYV 60 +LP P + T LA GE+ ++ SL RV GF +G A + LG+ +G+SR + + Sbjct: 52 LLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALL 111 Query: 61 YPLFKAFSQVPVLGWLPLLMLLVGIDEALKIILISKAALVPIALNTFKGIENVPARYVEV 120 P F+A +P L W+PLL+L +GIDE K++LI+ A P+ LN GI ++ + +EV Sbjct: 112 EPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEV 171 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 48/131 (36%), Positives = 72/131 (54%) Query: 1 VLPSPQSVLLTFIDLAASGEMFDNLKISLLRVLLGFGVGLAGGLLLGVAMGLSRVFKDYV 60 +LP P +VL +L+ASGE++ +L +S R GF VG GL+LG+ G R + + Sbjct: 60 ILPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLL 119 Query: 61 YPLFKAFSQVPVLGWLPLLMLLVGIDEALKIILISKAALVPIALNTFKGIENVPARYVEV 120 + +P L +PL++L GIDE K+ L+S P+ LNTF GI +V +E+ Sbjct: 120 DTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEM 179 Query: 121 ARVLRFSRWQL 131 A+ S W L Sbjct: 180 AKSYGLSGWPL 190 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 59.3 bits (142), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 38/111 (34%), Positives = 56/111 (50%) Query: 24 NLKISLLRVLLGFGVGLAGGLLLGVAMGLSRVFKDYVYPLFKAFSQVPVLGWLPLLMLLV 83 N+ SL RV GFG+ G+ LG A+G P+ V L WLP+ +L+ Sbjct: 112 NVLASLQRVGTGFGLAALVGIPLGFAIGRVEFLARMFGPIISLLRPVSPLAWLPIGLLVF 171 Query: 84 GIDEALKIILISKAALVPIALNTFKGIENVPARYVEVARVLRFSRWQLLTR 134 I I ++ P+ +NT G++ VP Y+ VARVL S W++LT+ Sbjct: 172 KSANPAAIWSIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKVLTK 222 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 581,264 Number of extensions: 16566 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 8 Length of query: 256 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 173 Effective length of database: 1,226,865 Effective search space: 212247645 Effective search space used: 212247645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)