BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0131.0009.p (190 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311997|ref|YP_003776089.1| TRAP-type C4-dicarboxylate tran... 276 7e-76 >gi|300311997|ref|YP_003776089.1| TRAP-type C4-dicarboxylate transport system small permease [Herbaspirillum seropedicae SmR1] Length = 192 Score = 276 bits (706), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 147/186 (79%), Positives = 164/186 (88%), Gaps = 1/186 (0%) Query: 1 MSHGFEMQPQAKPAGGDISANAVVAGLHHALQKFNKLAVFLAMIALMLTSIILTYSVVVR 60 MSHGFEM P A ++ A+A VA L L+ ++++AVFL MIAL++TS ILTYSVVVR Sbjct: 1 MSHGFEM-PGAAATRPEVPASAPVAWLGAFLRGYHRVAVFLGMIALLVTSCILTYSVVVR 59 Query: 61 YFFHQPTDWQDEASVFMLVGVIFLCSAYVQSLRGHIGIEALAGFLSPRANQVRMFIVDLI 120 YFFHQPTDWQDEASVFMLVGVIFLCSAYVQSLRGHIGIEALAG LSP AN+VRMFIVD++ Sbjct: 60 YFFHQPTDWQDEASVFMLVGVIFLCSAYVQSLRGHIGIEALAGMLSPLANRVRMFIVDVV 119 Query: 121 SFVFCAFFAWKSWTLFHEAWVDGQTTSSTFAPPLWIPYSMMALGMTVLTLQLLVQVLTRI 180 S +FCAFF WKSW LFHEAWV+GQTTSSTFAPPLWIPYS+MALGMT+LTLQLLVQVL RI Sbjct: 120 SLLFCAFFTWKSWALFHEAWVEGQTTSSTFAPPLWIPYSLMALGMTMLTLQLLVQVLVRI 179 Query: 181 TGTVPA 186 TGTVPA Sbjct: 180 TGTVPA 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 556,060 Number of extensions: 18916 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 1 Length of query: 190 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 110 Effective length of database: 1,241,070 Effective search space: 136517700 Effective search space used: 136517700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)