BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0130.0010.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309995|ref|YP_003774087.1| Type IV pilin A protein [Herbas... 127 5e-31 >gi|300309995|ref|YP_003774087.1| Type IV pilin A protein [Herbaspirillum seropedicae SmR1] Length = 168 Score = 127 bits (319), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 74/166 (44%), Positives = 97/166 (58%), Gaps = 17/166 (10%) Query: 5 TQSAQRGFTLIELMIVIAIVGVLAMVAIPQYRDYLIRTKVVEGLNLATPAKMAVSETINA 64 T+S+Q+GFTL+ELM+ +AIVG+LAM+ QY+DY+ R +V EG NLA P K+AV+E I++ Sbjct: 6 TRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQISS 65 Query: 65 KGGTNFKALDTGYSPPAATSTVASITISDADSAGNGGGVITIKYATIGGEGDGKTLILIP 124 G F G T+ V I++S G GGVITI Y+ GEG TL L P Sbjct: 66 NNGV-FTVAQLGLPAFTPTANVTGISVSPMQPRGV-GGVITITYSAQVGEGG--TLTLTP 121 Query: 125 AVGNSAIAWACHAAGATTNP-------------GTLPAKYAPESCK 157 + + I W C AAG T P GTLPAKYAP +C+ Sbjct: 122 TIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 517,479 Number of extensions: 21709 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 1 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)