BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0130.0004.p (63 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310001|ref|YP_003774093.1| hypothetical protein Hsero_0666... 96 6e-22 >gi|300310001|ref|YP_003774093.1| hypothetical protein Hsero_0666 [Herbaspirillum seropedicae SmR1] Length = 66 Score = 95.9 bits (237), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 49/66 (74%), Positives = 52/66 (78%), Gaps = 3/66 (4%) Query: 1 MSQVQQTQPV-QLDGKDLPAHCPNPNMPHWSSHPRVFLELDDHGAAKCP--GTQYQLKPG 57 MSQ Q +Q V L+GKDLPAHCP P M W+SHPRVFLELD HGAAKCP GTQYQLKPG Sbjct: 1 MSQAQASQGVVLLEGKDLPAHCPGPGMTTWNSHPRVFLELDAHGAAKCPYCGTQYQLKPG 60 Query: 58 AVVKAH 63 VVK H Sbjct: 61 TVVKGH 66 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 223,521 Number of extensions: 7288 Number of successful extensions: 17 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 16 Number of HSP's successfully gapped: 1 Length of query: 63 Length of database: 1,619,870 Length adjustment: 35 Effective length of query: 28 Effective length of database: 1,454,145 Effective search space: 40716060 Effective search space used: 40716060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)