BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0129.0020.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 731 0.0 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 125 9e-30 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 111 1e-25 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 109 3e-25 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 105 9e-24 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 731 bits (1886), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/401 (88%), Positives = 372/401 (92%), Gaps = 9/401 (2%) Query: 7 PRSFARIDRLPPYVFNITAELKMAARRRGEXGAELKMAARGGGGGGGNPDGATPPHIVEK 66 P SF RI+RLPPYVFNITAELKMAARRRGE ++ M GNPDGATPPHIVEK Sbjct: 38 PVSFPRIERLPPYVFNITAELKMAARRRGEDIIDMSM---------GNPDGATPPHIVEK 88 Query: 67 LVEVAQRPDTHGYSASKGIPRLRRAIAHWYKSRYDVEFNPDSEAIVTIGSKEGLAHLMLA 126 LVEVAQRPDTHGYS+SKGIPRLRRAIAHWY+SRY+V+F+PDSEAIVTIGSKEGLAHLMLA Sbjct: 89 LVEVAQRPDTHGYSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLA 148 Query: 127 TLDKGDTVLVPNPSYPIHIYGAVIAGADIRSVRMSPDVDFFSELERAIRESYPKPKMMVL 186 TLDKGDTVLVPNPSYPIHIYGAVIAGA+IRSVRMSP VDFF ELERA+RESYPKPKMM+L Sbjct: 149 TLDKGDTVLVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMIL 208 Query: 187 GFPSNPTAQCVELEFFERVIKLAREHNILVVHDLAYADITFDGWKAPSIMQVEGAREVAV 246 GFPSNPTAQCVELEFFERV+KLAREH ILVVHDLAYADITFDGWKAPSIMQV GAREVAV Sbjct: 209 GFPSNPTAQCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAV 268 Query: 247 EFFTLSKSYNMAGWRIGFMVGNKELVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVE 306 EFFTLSKSYNMAGWRIGFMVGN LVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVE Sbjct: 269 EFFTLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVE 328 Query: 307 EIRANYESRRNVLVKGLHEAGWMVDVPKASMYIWARIPEAYRHLGSLEFSRQLLDKAKVC 366 EIRANYE RRNVLVKGLHEAGWMVDVPKASMYIWARIPE YR GSLEF+R LL++AKVC Sbjct: 329 EIRANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVC 388 Query: 367 VSPGIGFGEYGDEYVRFALIENEARIRQAIRGIKSMLRGEK 407 VSPGIGFGEYGDEYVRFALIENE+RIRQAIRGIK+M + K Sbjct: 389 VSPGIGFGEYGDEYVRFALIENESRIRQAIRGIKAMFKNAK 429 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 125 bits (313), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 98/368 (26%), Positives = 174/368 (47%), Gaps = 25/368 (6%) Query: 13 IDRLPPYVFNITAELKMAARRRGEXGAELKMAARGGGGGGGNPDGATPPHIVEKLVEVAQ 72 +D+L PY F +L G A R G G P TP I + L++ Sbjct: 22 LDQLQPYPFEKLKKLF--------AGITPDPAYRPISLGIGEPKHPTPAFIQQALID--N 71 Query: 73 RPDTHGYSASKGIPRLRRAIAHWYKSRYDV-EFNPDSEAIVTIGSKEGLAHLMLATLDK- 130 Y A+ G LR AIA W + RYD+ +P +E + GS+E L L +D Sbjct: 72 LAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQTVVDPT 131 Query: 131 --GDTVLVPNPSYPIHIYGAVIAGADIRSVRMSPDVDFFSELERAIRESYPKPKMMVLGF 188 G V+ PNP Y I+ A +AGA V P +F + + ++ + + +++ + Sbjct: 132 RPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCS 191 Query: 189 PSNPTAQCVELEFFERVIKLAREHNILVVHDLAYADITFDGWKAPSIMQVEGAREVA--- 245 P NPT + LE ++ + L+ + ++ D Y++I F+ + P + ++ AR + Sbjct: 192 PGNPTGAVLTLEDWKELFALSDRYGFVIASDECYSEIYFN--EDPPLGGMQAARLLGRDY 249 Query: 246 ---VEFFTLSKSYNMAGWRIGFMVGNKELVAALARIKSYHDYGSFTPVQVAAIAALEGDQ 302 + F +LSK N+ G R GF+ G+ ++ ++YH +Q A++ A + D+ Sbjct: 250 RRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ-DE 308 Query: 303 SCVEEIRANYESRRNVLVKGLHEAGWMVDVPKASMYIWARIPEAYRHLGSLEFSRQLLDK 362 + V+ A Y ++ + + L E V +P A+ Y+WA++ + ++ EF+++L + Sbjct: 309 THVQGNIAKYVTKFSQVTPLLQEV-LEVALPDAAFYLWAKV-DKLVNISDTEFAQRLYAE 366 Query: 363 AKVCVSPG 370 V V PG Sbjct: 367 YNVTVLPG 374 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 111 bits (277), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 96/366 (26%), Positives = 172/366 (46%), Gaps = 19/366 (5%) Query: 51 GGGNPDGATPPHIVEKLVEVAQRPDTHG---YSASKGIPRLRRAIAHWYKSRYDVEFNPD 107 G G PD PP +VE A R T G Y+++ G+P+LR AI+ Y+S Y +E P+ Sbjct: 39 GIGEPDFTAPPAVVE----AATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEIAPE 94 Query: 108 SEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGADIRSVRMSPDVDFF 167 +VT G+ L A +++ VL+P+PSYP + + + + P+ F Sbjct: 95 -RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPE-HRF 152 Query: 168 SELERAIRESY-PKPKMMVLGFPSNPTAQCVELEFFERVIKLAREHNILVVHDLAYADIT 226 + +RE + + ++L PSNPT + + ++ R+ + D Y ++ Sbjct: 153 QLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVDEIYQGLS 212 Query: 227 FDGWKAPSIMQVEGAREVAVEFFTLSKSYNMAGWRIGFMVGNKELVAALARIKSYHDYGS 286 ++G AP G V + F SK +NM GWR+G++V LV + ++ + Sbjct: 213 YEG--APFSALSLGDDVVVINSF--SKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICA 268 Query: 287 FTPVQVAAIAALEGDQ-SCVEEIRANYESRRNVLVKGLHEAGWMVDV-PKASMYIWARIP 344 + Q AA+A + EE +A ++ RR+ +V L G+ V V P + Y++A Sbjct: 269 SSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFYVYADCS 328 Query: 345 EAYRHLGSLEFSRQLLDKAKVCVSPGIGFGEY-GDEYVRFALIENEARIRQAIRGIKSML 403 L S +L++A V + PG+ FG + Y+R + + +++A+ ++ Sbjct: 329 ALADDADQL--SLDMLNEAGVVLVPGLDFGPFTARRYIRLSYATSMENLQEAVARLRVFF 386 Query: 404 RGEKEA 409 ++A Sbjct: 387 EKRRKA 392 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 101/408 (24%), Positives = 184/408 (45%), Gaps = 18/408 (4%) Query: 8 RSFARIDRLPPYVFNITAELKMAARRRGEXGAELKMAARGGGGGGGNPDGATPPHIVEKL 67 R + +L ++I + AR+ E G ++ G G P IV+ + Sbjct: 2 RPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFG---FDAPDEIVQDM 58 Query: 68 VEVAQRPDTHGYSASKGIPRLRRAIAHWYKSRYDVEFNPDSEAIVTIGSKEGLAHLMLAT 127 + + GY+ SKG+ R+++ H Y + +E + + G+ E + + A Sbjct: 59 IR--NMGNASGYTDSKGLFAPRKSVMH-YTQQKKIEGVTIDDIYLGNGASELIVMSVNAL 115 Query: 128 LDKGDTVLVPNPSYPIHIYGAVIAGADIRSVRMSPDVDFFSELERAIRESYPKPKMMVLG 187 L+ GD VLVP+P YP+ ++G + ++ ++ P K +V+ Sbjct: 116 LNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVI 175 Query: 188 FPSNPTAQCVELEFFERVIKLAREHNILVVHDLAYADITFDGWKAPSIMQVEGAREVA-V 246 P+NPT +E + +I+LAR+H ++++ D Y + +DG S+ + A +V + Sbjct: 176 NPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASL--ADDVLFI 233 Query: 247 EFFTLSKSYNMAGWRIGFMVGNKELVAALARIKSYHDYGSF-----TPVQVAAIAALEGD 301 F LSK+Y G+R G+MV + E A I+ + S P Q A AL G Sbjct: 234 TFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGY 293 Query: 302 QSCVEEI--RANYESRRNVLVKGLHEA-GWMVDVPKASMYIWARI-PEAYRHLGSLEFSR 357 QS + + + +R++ + L + G PK+++Y++ R+ PE Y EF+ Sbjct: 294 QSINDLVGPQGRLTKQRDLAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAY 353 Query: 358 QLLDKAKVCVSPGIGFGEYGDEYVRFALIENEARIRQAIRGIKSMLRG 405 +LL + KV + G GF ++ R + N + +++ I L G Sbjct: 354 ELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDDLTESMGRIAHFLEG 401 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 85/355 (23%), Positives = 154/355 (43%), Gaps = 6/355 (1%) Query: 51 GGGNPDGATPPHIVEKLVEVAQRPDTHGYSASKGIPRLRRAIAHWYKSRYDVEFNPDSEA 110 G G PD P +V + + Q + Y G+P LR AIA + Y ++P SE Sbjct: 34 GQGFPDFHCDPALVAAVTQAMQE-GLNQYPPMAGVPVLREAIAEKVEKLYGHRYDPVSEI 92 Query: 111 IVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGADIRSVRMSPDVDFFS-E 169 VT G+ +G+ +L + GD V+V P Y ++ +AG V+M +S Sbjct: 93 TVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGAQGYSIP 152 Query: 170 LERAIRESYPKPKMMVLGFPSNPTAQCVELEFFERVIKLAREHNILVVHDLAYADITFDG 229 ++ PK +M+++ P NPT + + + R +IL++ D Y + +DG Sbjct: 153 WDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEHMVYDG 212 Query: 230 WKAPSIMQVEGAREVAVEFFTLSKSYNMAGWRIGFMVGNKELVAALARIKSYHDYGSFTP 289 S+ + E + + K+Y++ GW++G++ L A ++ ++ + TP Sbjct: 213 QPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFNVFTVNTP 272 Query: 290 VQVAAIAALEGDQSCVEEIRANYESRRNVLVKGLHEAGWMVDVPKASMYIWARIPEAYRH 349 VQ +AA D + ++ A Y+ +R++ GL + + +P Y A Sbjct: 273 VQY-GLAAYMKDPAPYLDLPAFYQHKRDLFRTGLANTRFEL-LPSQGTYFQCVKYGAISA 330 Query: 350 LGSLEFSRQLLDKAKVCVSPGIGFGEYGDE--YVRFALIENEARIRQAIRGIKSM 402 L EF + L + V P F E VRF + + ++ A+ + + Sbjct: 331 LPEAEFCKWLTSEIGVAAIPVSAFYNTPRESGIVRFCFAKKDETLQLALERLAKL 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,281,795 Number of extensions: 52124 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 12 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)