BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0129.0019.p (143 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311981|ref|YP_003776073.1| integral membrane protein [Herb... 165 2e-42 >gi|300311981|ref|YP_003776073.1| integral membrane protein [Herbaspirillum seropedicae SmR1] Length = 149 Score = 165 bits (417), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 83/141 (58%), Positives = 104/141 (73%), Gaps = 3/141 (2%) Query: 2 HILGFTVWVGGMFFAYLCLRPVAAAVLQPPARLALWNGVFGKFFTWVWLAIALILASGLP 61 H+LG T+WVGGMFFA++ LRP A AVL P RL L V FF WVW+AIAL+L SGL Sbjct: 8 HLLGLTIWVGGMFFAHMALRP-ALAVLHPEQRLTLMAAVLASFFVWVWIAIALVLGSGLH 66 Query: 62 MMAQMG--KPPVYISGMAGLGILMMLIFAHLFFAPYRRLKRAVAAEDWAAGGAALGQIRR 119 MMA +G P Y+ M G+G++MMLIFAH+FFAP+RRLK A A +DW ALGQ+R+ Sbjct: 67 MMAMLGAGHAPPYVHAMTGIGVVMMLIFAHIFFAPFRRLKLAAAQQDWDRAARALGQVRQ 126 Query: 120 IVGINLILGLIVVTLGALGPL 140 +V INL+LGL+ + +GALGPL Sbjct: 127 LVSINLLLGLLTIAIGALGPL 147 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.147 0.480 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 387,687 Number of extensions: 15576 Number of successful extensions: 82 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 80 Number of HSP's successfully gapped: 1 Length of query: 143 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 66 Effective length of database: 1,255,275 Effective search space: 82848150 Effective search space used: 82848150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 126 (53.1 bits)