BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0025.p (412 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 748 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 214 9e-57 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 102 5e-23 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 748 bits (1930), Expect = 0.0, Method: Compositional matrix adjust. Identities = 355/413 (85%), Positives = 383/413 (92%), Gaps = 1/413 (0%) Query: 1 MNEKKNYGFTTTILHSDRQKTIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRYGR 60 MN+KK YGFTTTILHSDRQK IEHGSLHKPIHTSV FGY+DARQLAEVFQGKQ GYRYGR Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 61 QGNPTVSALEDKVTGMEGGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNSMW 120 QGNPTV+ALEDK+T ME G STICFATGMAAIGA++QGLLREGDHVVSSAFLFGNTNS+W Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 121 MTVHAQGARVSMVDATDVKLVEEALTPQTRIVFVETIANPRTQIADLKRIGELCRERGIL 180 MTV AQGA+VSMVDATDVK VE A+T TR+VFVETIANPRTQ+ADLKRIGELCRERGIL Sbjct: 121 MTVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGIL 180 Query: 181 YIVDNTMTSPYLFQPKAVHAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHIADNY 240 Y+VDNTMTSPYLF+PK V AGLVVNSLTKSIGGHGNALGGALTDTG +DW+RYPHIA+NY Sbjct: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENY 240 Query: 241 KKNPQAQWGMAQIRX-ILRDFGASLGPEAAHHIAVGAETIALRQDRECANALAVAQMLDA 299 KKNP QWGMAQIR LRDFG SLGPEAAHHIAVGAETIALRQ+REC NALA+AQML A Sbjct: 241 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA 300 Query: 300 DPRVAAVYYPGLPSHPQHALATELFRSYGSLLSFELKDGIDCFDYLNRLQLAVASSNLGD 359 D RVAAVYYPGL SHPQHAL+ LFRS+GSL+SFELKDGIDCFDYLNRL+LA+ +SNLGD Sbjct: 301 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD 360 Query: 360 TRTLVIPVAHTIFFEMGQERRAAMGIAESLIRVSIGLEDTDDLVEDFRQALDS 412 TRTLVIPVAHTIF+EMG ERRA+MGIAESLIRVS+GLEDTDDLV DFRQALD+ Sbjct: 361 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 214 bits (546), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 141/428 (32%), Positives = 220/428 (51%), Gaps = 16/428 (3%) Query: 1 MNEKKNYGFTTTILHSDRQKTIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRYGR 60 M + + F T +H ++ PI+ +VAF + D + A++F K G Y R Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 61 QGNPTVSALEDKVTGMEGGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNSMW 120 NPT LE ++ +EGG+ + A+G AA+ IQ + GD++VS++ L+G T +++ Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 121 M-TVHAQGARVSMVDATDVKLVEEALTPQTRIVFVETIANPRTQIADLKRIGELCRERGI 179 T+ G + D E + +T+ +++E+I NP I D+ + ++ G+ Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 180 LYIVDNTMTSPYLFQPKAVHAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHIADN 239 IVDNT+ +PYL +P A +VV+SLTK +GGHG LGGA+ D+G + W+++ Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 Query: 240 YKKNPQAQWGMAQIRXI-------------LRDFGASLGPEAAHHIAVGAETIALRQDRE 286 + + G+ + LR+ GA+L P A I G ET+ LR DR Sbjct: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 Query: 287 CANALAVAQMLDADPRVAAVYYPGLPSHPQHALATELFRSYGS-LLSFELKDGID-CFDY 344 AN LAVAQ L P+V V Y GL HP HALA F+ S +L+F + +G + + Sbjct: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF 360 Query: 345 LNRLQLAVASSNLGDTRTLVIPVAHTIFFEMGQERRAAMGIAESLIRVSIGLEDTDDLVE 404 + LQL N+GD ++L A T ++ G+ E +R+SIG+E DDL+ Sbjct: 361 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA 420 Query: 405 DFRQALDS 412 D QAL S Sbjct: 421 DLEQALQS 428 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 102 bits (254), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 102/424 (24%), Positives = 175/424 (41%), Gaps = 49/424 (11%) Query: 1 MNEKKNYGFT--TTILHSDRQKTIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRY 58 M++ K T T+++HS + + IH + + D + ++ GY Y Sbjct: 1 MSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 Query: 59 GRQGNPTVSALEDKVTGMEGGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNS 118 G G PT LE ++ +EGG +G+AAI V L+ GD V+ ++G + Sbjct: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 Query: 119 M--WMTVHAQGARVSMVDATDVKLVEEALTPQTRIVFVETIANPRTQIADLKRIGELCRE 176 + W+ Q D + + E + P TR+V+ E + ++ D+ + Sbjct: 121 LGQWLERDFQ-ITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHA 179 Query: 177 RGILYIVDNTMTSPYLFQPKAVHAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHI 236 +G+L +DNT ++ F+ +V+ ++TK G + L GA+ I Sbjct: 180 KGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------I 226 Query: 237 ADNYKKNPQAQWGMAQIRXILRDFGASLGPEAAHHIAVGAETIALRQDRECANALAVAQM 296 + N + + +A +R G +G + + + ++ LR + A A VA Sbjct: 227 VRDQALNEKLE--LAHMR-----LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAW 279 Query: 297 LDADPRVAAVYYPGLPSHPQHALATELFRSYGSLLSFELKDGIDCFD----------YLN 346 L P + AV +P P HA F G L S FD +++ Sbjct: 280 LKQRPEIVAVLHPAFEDCPGHAHWKRDFSGAGGLFSV-------IFDPRYVEAQTDRFVD 332 Query: 347 RLQLAVASSNLGDTRTLVIPVAHTIFFEMGQERRAAMGIAESLIRVSIGLEDTDDLVEDF 406 L+L + G +L +P + M RRA M + L+R +IGLE +DL+ D Sbjct: 333 SLRLFKIGYSWGGAHSLCVP------YRMKGMRRAWMREGQ-LVRFNIGLESPEDLIADI 385 Query: 407 RQAL 410 QAL Sbjct: 386 EQAL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,306,195 Number of extensions: 55226 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 3 Length of query: 412 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 324 Effective length of database: 1,203,190 Effective search space: 389833560 Effective search space used: 389833560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)