BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0127.0013.p (392 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 368 e-103 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 273 2e-74 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 173 2e-44 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 91 2e-19 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 67 2e-12 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 368 bits (945), Expect = e-103, Method: Compositional matrix adjust. Identities = 175/190 (92%), Positives = 184/190 (96%) Query: 1 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLA 60 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETL+RPTAML+RNLA Sbjct: 45 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLA 104 Query: 61 SMDVEESIRANPLDGVVLLMGCDKTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGS 120 SMDVEESIRANPLDGVVLLMGCDKTTPALLMGA+SVD+PTIG+SGGPMLSGKYRG ELGS Sbjct: 105 SMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGS 164 Query: 121 GTGVWQMSEEVRGGHMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAA 180 GTGVWQMSE+VR G M+QEEFFEAESCMHRSHGHCMTMGTASTMASMVEALG+ LP NAA Sbjct: 165 GTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAA 224 Query: 181 IPAVDARRXI 190 IPAVDARR + Sbjct: 225 IPAVDARRNV 234 Score = 311 bits (796), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 149/174 (85%), Positives = 160/174 (91%), Gaps = 5/174 (2%) Query: 181 IPAVDARRXIRELESVIDKSALSANGQTLWDNCKDAPNWNRDVIHAFDTPFKAAAGIAIL 240 +PAV IR+LESVIDK+AL+ANG+TLW+NC+DAPNWN +VI +FD PFK AAGIAIL Sbjct: 328 LPAV-----IRQLESVIDKTALTANGKTLWENCQDAPNWNEEVIRSFDKPFKEAAGIAIL 382 Query: 241 HGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRIDDENLDVDENCVLVLKNCGP 300 GNL PDGAVIKPSAATPALLKH GRAVVFENSDDLHKRIDDENLDVDE CVLVLKNCGP Sbjct: 383 KGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGP 442 Query: 301 KGYPGMGEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVTPEAAAGG 354 KGYPGM EAGNMPLPPK+LRKGITDMVRVSDARMSGTAYGTVVLHV+PEAAAGG Sbjct: 443 KGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGG 496 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 273 bits (697), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 132/190 (69%), Positives = 159/190 (83%) Query: 1 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLA 60 VIGICNT+SELTPCN+HFR +AE V+ GI EAGGFP+EFPV S GE+ +RPTAML RNLA Sbjct: 47 VIGICNTWSELTPCNAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLA 106 Query: 61 SMDVEESIRANPLDGVVLLMGCDKTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGS 120 SMDVEESIR NP+D VVLL GCDKTTPALLMGA+S D+P I ++GGPML+GK++G ++GS Sbjct: 107 SMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGS 166 Query: 121 GTGVWQMSEEVRGGHMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAA 180 GT VWQ+SE+V+ G ++ +F AE+ M RS G C TMGTASTMA M E+LG+SLP NAA Sbjct: 167 GTVVWQLSEQVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAA 226 Query: 181 IPAVDARRXI 190 IPAVDARR + Sbjct: 227 IPAVDARRYV 236 Score = 221 bits (563), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 109/174 (62%), Positives = 130/174 (74%), Gaps = 2/174 (1%) Query: 181 IPAVDARRXIRELESVIDKSALSANGQTLWDNCKDAPNWNRDVIHAFDTPFKAAAGIAIL 240 +PAV R + E + + K AL+ NGQT+WDN KDAP +N +V+ P GI IL Sbjct: 330 LPAV--LRRLGEADLLPHKDALTVNGQTMWDNVKDAPIYNDEVVRPLAKPLIEDGGICIL 387 Query: 241 HGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRIDDENLDVDENCVLVLKNCGP 300 GNL P GAV+KPSAATP L+KH GRAVVFE+ + +RI+D +LDVD +CVLV+KN GP Sbjct: 388 RGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGP 447 Query: 301 KGYPGMGEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVTPEAAAGG 354 KGYPGM E GNM LPPKVL G+ DMVR+SDARMSGTAYGTV+LHV PEAAAGG Sbjct: 448 KGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEAAAGG 501 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 173 bits (438), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 88/183 (48%), Positives = 122/183 (66%) Query: 1 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLA 60 +IGI + S+++PCN LA++V+ GI +AGG P+EFP+ + E RPTA + RNLA Sbjct: 49 IIGIAQSGSDISPCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLA 108 Query: 61 SMDVEESIRANPLDGVVLLMGCDKTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGS 120 + + E + P+D VVL GCDKTTP+ +M A++VD+P I +SGGPML G G +GS Sbjct: 109 YLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGS 168 Query: 121 GTGVWQMSEEVRGGHMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAA 180 G+ +W+ + + G + E+F E + S GHC TMGTASTM +M EALGMSL G +A Sbjct: 169 GSAIWKGRKLLSAGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSA 228 Query: 181 IPA 183 IPA Sbjct: 229 IPA 231 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 58/164 (35%), Positives = 94/164 (57%), Gaps = 12/164 (7%) Query: 202 LSANGQTLWDNCKDAPNWNRDVIHAFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPAL- 260 ++A G+T+ +N + + +R++I+ F P + AG +L GNL D A++K S + Sbjct: 350 ITATGKTMAENLQGRASNDREMIYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVISETFR 408 Query: 261 ---LKHTG-------RAVVFENSDDLHKRIDDENLDVDENCVLVLKNCGPKGYPGMGEAG 310 L G RAVVF+ SDD H RI+D L +DEN +L ++ GP G+PG E Sbjct: 409 ERYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVV 468 Query: 311 NMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVTPEAAAGG 354 NM P ++++G++ + + D R SGT+ +L+ +PE+A GG Sbjct: 469 NMQPPDALIKRGVSTLPTLGDGRQSGTSDSPSILNASPESAVGG 512 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 60/198 (30%), Positives = 101/198 (51%), Gaps = 11/198 (5%) Query: 1 VIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLA 60 +I + N++++ P + H + L + V I AGG EF +++ ++ A + +L Sbjct: 36 IIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLP 95 Query: 61 SMD-----VEESIRANPLDGVVLLMGCDKTTPALLMGASSVDLPTIGISGGPMLSGKYRG 115 S D VE + A+ D +V + CDK TP +LM A +++P + +SGGPM +GK Sbjct: 96 SRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVI- 154 Query: 116 GELGSGTGVWQM---SEEVRGG--HMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVEA 170 + + V ++ ++ G ++S + E E + G C M TA++M + E Sbjct: 155 KTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCLTEV 214 Query: 171 LGMSLPGNAAIPAVDARR 188 LG+SLPGN I A A R Sbjct: 215 LGLSLPGNGTIVATHADR 232 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 46/136 (33%), Positives = 74/136 (54%), Gaps = 11/136 (8%) Query: 221 RDVIHAFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRI 280 RD HA+ G+A+L+GN+ G ++K + ++LK +G A VFE+ D + I Sbjct: 414 RDREHAYSQD----GGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGI 469 Query: 281 DDENLDVDENCVLVLKNCGPKGYPGMGEAGNMPLPPKVLR-KGITDMVRV-SDARMSGTA 338 + + + V++++ GPKG PGM E M P ++ KG+ + +D R SG + Sbjct: 470 LGDKVKAGD--VVIVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGS 524 Query: 339 YGTVVLHVTPEAAAGG 354 G V+ H +PEAA GG Sbjct: 525 SGLVIGHASPEAAEGG 540 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 52/187 (27%), Positives = 84/187 (44%), Gaps = 14/187 (7%) Query: 2 IGICNTYSELTPCNSHFRALAEQVKIGIWEAGG---FPLEFPVMSLGETLMRP---TAML 55 + I + Y+++ + F + +K EAG F P M G T + ++ Sbjct: 70 LAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGMELSLF 129 Query: 56 FRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGASSV-DLPTIGISGGPMLSGKYR 114 R+ +M ++ N D + L CDK P LL+GA LP + + GPM SG Sbjct: 130 SRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGM-- 187 Query: 115 GGELGSGTGVWQMSEEVRGGHMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGMS 174 S + ++ + G + + E EAES + G C GTA++ ++EA+G+ Sbjct: 188 -----SNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLH 242 Query: 175 LPGNAAI 181 LPG A I Sbjct: 243 LPGAAFI 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,266,363 Number of extensions: 54038 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 9 Length of query: 392 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 305 Effective length of database: 1,207,925 Effective search space: 368417125 Effective search space used: 368417125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)