BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0127.0003.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 463 e-132 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 127 2e-30 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 116 2e-27 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 113 2e-26 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 100 2e-22 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 463 bits (1192), Expect = e-132, Method: Compositional matrix adjust. Identities = 218/309 (70%), Positives = 258/309 (83%) Query: 1 MGMSDFYSNRDDNESIATIHRALELGICFLDTADMYGPYINEELIAKAIKGKRDQVFIAT 60 MGMSDFY+ RDD ES+ATI RALELGI LDTADMYGPY NEEL+ +AIKG+RD+ FIAT Sbjct: 35 MGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRAIKGRRDKFFIAT 94 Query: 61 KFGIVRDPANPAARGVNGKPDYIRTSVEGSLKRLGIEAIDLYYQHRIDQNTPIEDTVGAL 120 KFGI RDP++P ARGV+G P YIR +VEGSLKRLG+E IDLYYQHRID TPIE T+G L Sbjct: 95 KFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVL 154 Query: 121 ADLVKAGKIRHIGLSEASSQTLERASKIHKITALQSEYSLWTRDPERDVLATCKKLGIGF 180 ADLV+AGKIR+IGLSEAS+ TLERA ++H +TALQSEYSLWTRDPE +VLA C+ LGIGF Sbjct: 155 ADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGF 214 Query: 181 VPYSPLGRGFLTGAITRPDQFDADDYRRHNPRFQGENFAKNLLLVEKVKELASRYACSPS 240 V YSPLGRGFLTGAI R + DD+RR NPRFQGENFA+NL L EKVKE+A ++ C+PS Sbjct: 215 VAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNLQLAEKVKEMAGQHGCTPS 274 Query: 241 QLALAWVLAQGEHIVPIPGTKRRKYLEENSGALAVTLTAAHLEELNRLFPADAASGPRYT 300 QLALAWV+AQ HIVPIPGTKRR+YLE+N+G++ V L L+ L+ +FP AA+G RYT Sbjct: 275 QLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQALDAIFPRGAAAGERYT 334 Query: 301 EEGMRILNG 309 + M++LNG Sbjct: 335 DASMKMLNG 343 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 95/317 (29%), Positives = 147/317 (46%), Gaps = 27/317 (8%) Query: 1 MGMSDFYSNRDDNESIATIHRALELGICFLDTADMYGPYINEELIAKAIKGKRDQVFIAT 60 +G F D ES + A E G+ F+DTAD+Y +EE++ K + G+R + +A+ Sbjct: 18 LGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEEIVGKLLAGQRHEWVLAS 77 Query: 61 KFGIVRDPANPAARGVNGKPD-------YIRTSVEGSLKRLGIEAIDLYYQHRIDQNTPI 113 K G ++GKP+ +I E L RL + +D+ Y HR Q+ + Sbjct: 78 KLG----------NAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHRDYQDENL 127 Query: 114 EDTVGALADLVKAGKIRHIGLSE------ASSQTLERASKIHKITALQSEYSLWTRDPER 167 E+ V AL DL++ GKIR G+S A L + + Q Y+L R PE Sbjct: 128 EEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEV 187 Query: 168 DVLATCKKLGIGFVPYSPLGRGFLTGAITRPDQFDADDYR--RHNPRFQGENF-AKNLLL 224 ++L C G+G VPYSP+ RG LTG +P Q A D R R + R F ++LL+ Sbjct: 188 EILPACAHYGLGVVPYSPIARGVLTGKY-QPGQKPAQDTRAGRGDKRMMETEFREESLLI 246 Query: 225 VEKVKELASRYACSPSQLALAWVLAQGEHIVPIPGTKRRKYLEENSGALAVTLTAAHLEE 284 +++++ Q A AWVLA I G + +E+ A + ++A Sbjct: 247 AQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISAEEEAL 306 Query: 285 LNRLFPADAASGPRYTE 301 ++ L AS Y + Sbjct: 307 VDSLVTPGHASTHGYND 323 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 84/287 (29%), Positives = 144/287 (50%), Gaps = 28/287 (9%) Query: 2 GMSDFYSNRDDNESIATIHRALELGICFLDTADMYGPYINE------ELIAKAIKGKRDQ 55 G+ +F + R A + RA + G+ D A+ YGP +AK RD+ Sbjct: 39 GVDNFETGR------AVLRRAFDRGVTHFDLANNYGPPAGSAEENFGRWMAKDFLPYRDE 92 Query: 56 VFIATKFGIVRDPANPAARGVNGKPDYIRTSVEGSLKRLGIEAIDLYYQHRIDQNTPIED 115 + I++K G P G +G ++ S E SLKR+G++ +D++Y HR+D +TP+E+ Sbjct: 93 LVISSKAGWQMWPGPYG--GPSGARKHLIASCEQSLKRMGLDYVDIFYSHRVDADTPLEE 150 Query: 116 TVGALADLVKAGKIRHIGLSEASSQTLERASKIHKITAL-----QSEYSLWTRDPERDVL 170 T+GALA L + GK ++G+S S + +A+ I K + Q YS+ R E +L Sbjct: 151 TMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPNYSMLNRAIEGGLL 210 Query: 171 ATCKKLGIGFVPYSPLGRGFLT----GAITRPDQFDADDYRRHNPRFQGENFAKNLLLVE 226 ++LG+G + +SPL +G LT G + P+ R E+ NL + Sbjct: 211 PALEELGVGCIGFSPLAQGLLTSKYLGGV--PEGARVTRAPSLPQRMLSED---NLARIR 265 Query: 227 KVKELASRYACSPSQLALAWVLAQGEHIVPIPGTKRRKYLEENSGAL 273 + E+AS+ + +Q+A+AWVL + G + + L+++ A+ Sbjct: 266 ALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAV 312 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 91/300 (30%), Positives = 150/300 (50%), Gaps = 16/300 (5%) Query: 7 YSNRDDNESIATIHRALELGICFLDTADMYGPYINEELIAKAIKGKR-DQVFIATKFGIV 65 + + ++ A +H AL+ G+ F+DTAD+YG +E+LIA+ +K + ++ F+ATK G Sbjct: 27 WGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQVLKERGGERPFVATKAG-- 84 Query: 66 RDPANPAARGVNGKPDYIRTSVEGSLKRLGIEAIDLYYQHRIDQNTPIEDTV-GALADLV 124 R + A G N + + ++ SLK LG++ +DL H + V G L LV Sbjct: 85 RRLSPHVASGYNDRAQ-LSAFIDRSLKNLGVDCLDLVQLHCPPTEVYYQQEVFGHLDALV 143 Query: 125 KAGKIRHIGLSEASSQTLERASKIHKITALQSEYSLWTRDPERDVLATCKKLGIGFVPYS 184 AGKIRH G+S + +A + + ++Q ++++ + P L ++ I + Sbjct: 144 AAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIFRQRPISLFLQEAQRRDIAVIARV 203 Query: 185 PLGRGFLTGAITRPDQFDADDYR---RHNPRFQ-GENFAK-----NLLLVEKVKELASRY 235 PL G LTG ++ F ADD+R RH F GE F+ L VEK++ L + Sbjct: 204 PLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ- 262 Query: 236 ACSPSQLALAWVLAQGEHIVPIPGTKRRKYLEENSGALAV-TLTAAHLEELNRLFPADAA 294 +Q AL W+L + IPG + + NS A A+ +TA ++ L ++ D A Sbjct: 263 GVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALPPITAEVMQALRAIYLHDIA 322 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 83/281 (29%), Positives = 128/281 (45%), Gaps = 49/281 (17%) Query: 13 NESIATIHRALELGICFLDTADMYGPYINEELIAKAIKGKRDQVFIATKFGIVRDPANPA 72 N + + L+LG+ +DTA+MY E ++AKAI G+RDQV++ +K P N + Sbjct: 33 NAEVRALQAGLDLGMSLIDTAEMYAEGGAERIVAKAIAGRRDQVYLVSKV----LPHNAS 88 Query: 73 ARGVNGKPDYIRTSVEGSLKRLGIEAIDLYYQHRIDQNTPIEDTVGALADLVKAGKIRHI 132 RG + E SLKRLG + IDLY H + P+ T+ + LV GKI Sbjct: 89 KRGTI-------AACEASLKRLGTDRIDLYLLHWRGAH-PLAATIEGMETLVSQGKIGGW 140 Query: 133 GLSEASS----QTLERASKIHKITALQSEYSLWTRDPERDVLATCKKLGIGFVPYSPLGR 188 G+S + LE H + Q Y+L R E D+L ++ I + YSP+ + Sbjct: 141 GVSNFDTDDIDDLLEEPHGEHYLCN-QVLYNLSRRGIEYDLLPQAQQAHIAVMAYSPIEQ 199 Query: 189 GFLTGAITRPDQFDADDYRRHNPRFQGENFAKNLLLVEKVKELASRYACSPSQLALAWVL 248 G +L + LA + +P+Q+ALAWVL Sbjct: 200 G-------------------------------RILKHPALTALAQAHRVTPAQIALAWVL 228 Query: 249 AQGEHIVPIPGTKRRKYLEENSGALAVTLTAAHLEELNRLF 289 Q +V IP +++ N +L ++L+AA L+ L+ F Sbjct: 229 RQ-PGVVAIPKAADVAHVQANRASLDISLSAADLKILDEAF 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 967,235 Number of extensions: 39321 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 7 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)