BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0002.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 437 e-124 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 191 1e-49 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 65 7e-12 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 437 bits (1124), Expect = e-124, Method: Compositional matrix adjust. Identities = 215/271 (79%), Positives = 235/271 (86%), Gaps = 2/271 (0%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSAPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 +HLAAFLIAGNAAHSQ LWRHP+S GGFL LDYY IA+TLERGKFDLLFFADRLAVST Sbjct: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTTHIGLGATRSTTYSQPYSLAREFSTLD 122 R+G SH +G+ GDQDATR+DPLP+LGA+AA T+HIGLGATRSTTYSQPYSLAREF+TLD Sbjct: 61 RYGDSHLHGLYIGDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD 120 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 HLS GRAAWNVVTSVNQGEADNFGL+ETL HD RYDRADEFLEVTHKLW+SW AL L+ Sbjct: 121 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ 180 Query: 183 SGSGRYADPDGVHAIDHRGTHFNVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 RYAD D V AIDH+G HF+VRGPLNIPASPQG PVIIQAGSS RGQDFAARWAEVV Sbjct: 181 --QDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVV 238 Query: 243 FGIQPDLARMQRFYQDLKGRTGKFGRQPXKI 273 F IQPDL R+Q FY+DLK R +FGR+P I Sbjct: 239 FNIQPDLPRLQAFYRDLKNRAQQFGRRPQDI 269 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 191 bits (485), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 117/280 (41%), Positives = 149/280 (53%), Gaps = 18/280 (6%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSAPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 I L AF + + + LW HPR + + Y+ +A+ LERGKFD LF AD + V Sbjct: 6 IRLNAFNMNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGV-- 63 Query: 63 RFGGSHRYGVAHGDQDATR--------MDPLPVLGAIAAVTTHIGLGATRSTTYSQPYSL 114 Y V G+ D T DPL ++ A+AAVT H+G G T + TY P+ Sbjct: 64 -------YDVYQGNTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLF 116 Query: 115 AREFSTLDHLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWN-S 173 AR STLDHLS GR WN+VT A GL E L HD+RYDRADEF+EV +KLW S Sbjct: 117 ARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGS 176 Query: 174 WDAQALLLESGSGRYADPDGVHAIDHRGTHFNVRGPLNIPASPQGGPVIIQAGSSHRGQD 233 W+ A+L + YADP VH I H G +++V G S Q PV+ QAGSS RGQ Sbjct: 177 WEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQA 236 Query: 234 FAARWAEVVFGIQPDLARMQRFYQDLKGRTGKFGRQPXKI 273 F AR AE VF ++ Q ++ + GR P I Sbjct: 237 FGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAI 276 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 68/261 (26%), Positives = 101/261 (38%), Gaps = 54/261 (20%) Query: 18 QVLWRHPRSAPGGFL---------TLDYYKRIAQTLERGKFDLLFFADRLAVSTRFGGSH 68 ++ W P S +L TL+Y ++IA T E +D L Sbjct: 4 ELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIP------------- 50 Query: 69 RYGVAHGDQDATRMDPLPVLGAIAAVTTHIGLGATRSTTYSQPYSLAREFSTLDHLSAGR 128 A+ +DP V ++ VT I L T+ P AR+ +TLD GR Sbjct: 51 --------TGASCLDPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGR 102 Query: 129 AAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLESGSGRY 188 NVV + E + G+ L HDERY+ ADE+L + KL Sbjct: 103 LLLNVVPGGDATELEADGV--FLKHDERYEAADEYLTIWRKLLQG--------------- 145 Query: 189 ADPDGVHAIDHRGTHFNVR-GPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVVFGIQP 247 +++ G HF +R G L PA Q P + GSS D AA+ + Sbjct: 146 ------ETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 Query: 248 DLARMQRFYQDLKGRTGKFGR 268 A +++ D++ R + GR Sbjct: 200 PPAAVRQKIDDVRERAARQGR 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,203,642 Number of extensions: 51401 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 4 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)