BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0001.p (125 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 125 1e-30 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 59 1e-10 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 125 bits (313), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 58/83 (69%), Positives = 68/83 (81%) Query: 42 LPLADIGRLYAGGVLVPQVAGTAAEVADWMENIVAQQGADGFVITPAHLPEGFDDFVDLV 101 L LAD GRLYA GVL PQ+AGTAA+VADW+++I+ GADGFVI+P+HLP GFDDFVD V Sbjct: 347 LTLADAGRLYAQGVLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQV 406 Query: 102 VPELQRRGQFRLDYEGDTLRSYL 124 +PELQRRG FR DY G LR +L Sbjct: 407 IPELQRRGAFRQDYAGRQLRQHL 429 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 58.9 bits (141), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 29/65 (44%), Positives = 40/65 (61%) Query: 60 VAGTAAEVADWMENIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQFRLDYEGDT 119 + G VAD +E+ VA G DG+ +T PE F+DFV+LV+P LQ RG ++ +Y T Sbjct: 370 IVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGT 429 Query: 120 LRSYL 124 LR L Sbjct: 430 LREKL 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.141 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 475,262 Number of extensions: 20907 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 2 Length of query: 125 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 50 Effective length of database: 1,264,745 Effective search space: 63237250 Effective search space used: 63237250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 125 (52.8 bits)