BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0006.p (366 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 588 e-169 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 340 8e-95 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 326 2e-90 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 262 4e-71 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 244 9e-66 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 588 bits (1516), Expect = e-169, Method: Compositional matrix adjust. Identities = 292/367 (79%), Positives = 330/367 (89%), Gaps = 1/367 (0%) Query: 1 VTFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYSSIGLWA 60 +TFATFGVSFFMRPLGAVVLGAYSDR GRKAAL LSI+LMT+GTAMIAFMP+Y+SIGL A Sbjct: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA 120 Query: 61 PAGIVLARLIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGLSLLMAAVFGAGLN 120 PAGI L ++IQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQG+SLL+AA+FGA LN Sbjct: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 121 GLLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEFA-AAERTETPLRDTFSGQKM 179 +LTPEQL SWGWR+PF+FGLLI PAG YIRRHLDE+PEF ++E+T PLRDTF+ QKM Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 Query: 180 RLLIGTGSVIMATVSVYLALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVASPLVGELSD 239 RLLIG GSVIMATVSVYL+LY+PTYA+KQLG+PAWSS+AA V+GLIM + SPLVG LSD Sbjct: 241 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 Query: 240 KYGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADV 299 K GRTPFMI SS ++V TYPMFVFLT++P F+ LLLLQ VIGVLMTMYFA+MPALLAD+ Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 Query: 300 FPVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQATGNKLSVSFFVIFGAALSFIASTAA 359 FPV TRGTGMSL+YNIAV +FGGFAGLIITWLI TG+KLSVS+FVIFGA LS IAS +A Sbjct: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA 420 Query: 360 RYVLRVR 366 R VLR+R Sbjct: 421 RLVLRLR 427 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 340 bits (873), Expect = 8e-95, Method: Compositional matrix adjust. Identities = 176/360 (48%), Positives = 247/360 (68%), Gaps = 3/360 (0%) Query: 4 ATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYSSIGLWAPAG 63 ATFGV FFMRP+G ++LG Y+DRVGRKAALS++I+LM +GT MI PTY SIGL+AP Sbjct: 66 ATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLI 125 Query: 64 IVLARLIQGFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGLSLLMAAVFGAGLNGL 122 IV+ARL+QGFSAGGE GS+TAFL E+AP R R ++SSW AS G+++L+ A G + Sbjct: 126 IVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTAS 185 Query: 123 LTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEF-AAAERTETPLRDTFSGQKMRL 181 L L SWGWRLPFL G++IGP GYYIR HL+E+P F ++T++PL++ Sbjct: 186 LDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRET 245 Query: 182 LIGTGSVIMATVSVYLAL-YMPTYAIKQLGMPAWSSYAATIVSGLIMMVASPLVGELSDK 240 VI+ TV Y+ L YMPTY++K L +P + + A +V GL +MV +P+VG L+D+ Sbjct: 246 SASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADR 305 Query: 241 YGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADVF 300 GR P + GS+ ++ YPMF ++ SAPS LL+ Q+V G L+ Y + A +++F Sbjct: 306 IGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELF 365 Query: 301 PVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQATGNKLSVSFFVIFGAALSFIASTAAR 360 P + TG+S++YN+AV +FGGFA ITWLI ATG+ ++ +F+V+ AA+SF+ + R Sbjct: 366 PAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVR 425 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 326 bits (836), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 166/366 (45%), Positives = 249/366 (68%), Gaps = 6/366 (1%) Query: 1 VTFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYSSIGLWA 60 V ATFG+ FFMRP+G V+LG Y+DR GRKAA+ + IVLMT+ A+IAF PTY++IG+ A Sbjct: 51 VALATFGIGFFMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAA 110 Query: 61 PAGIVLARLIQGFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGLSLLMAAVFGAGL 119 P IV+AR++QGF+ GGE+ SSTAFLVE AP H+RG + SWQ+ Q L++ A GA + Sbjct: 111 PIMIVVARMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALV 170 Query: 120 NGLLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEF--AAAERTETPLR--DTFS 175 L+PE L SWGWRLPF+ GLLIGP G +IRRH+ E+ +F +A + P++ D Sbjct: 171 TRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVK 230 Query: 176 GQKMRLLIGTGSVIMATVSVYLAL-YMPTYAIKQLGMPAWSSYAATIVSGLIMMVASPLV 234 +L+ G VI TV+ Y+ L MPT+A KQLG+P ++ +++ ++ P Sbjct: 231 TNLRGVLVSMGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFA 290 Query: 235 GELSDKYGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPA 294 G LSD+ GR ++ SSLAF++ YP+FV++++APS LL++Q+++ +++ F MP Sbjct: 291 GALSDRLGRRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPT 350 Query: 295 LLADVFPVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQATGNKLSVSFFVIFGAALSFI 354 L++ FP + R TG++++YN+A MLFGGFA I+TWL + +G+ ++ +++V+F A + Sbjct: 351 ALSEQFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVS 410 Query: 355 ASTAAR 360 A+ A R Sbjct: 411 AAWAMR 416 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 262 bits (669), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 153/344 (44%), Positives = 219/344 (63%), Gaps = 9/344 (2%) Query: 2 TFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYSSIGLWAP 61 TF TF F +RP+GA+VLG Y+D+VGRK ALS +++LM IGT + A P+Y SIG+ A Sbjct: 58 TFMTFAAGFVVRPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAAT 117 Query: 62 AGIVLARLIQGFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGLSLLMAAVFGAGLN 120 +V RL+QGFSAGGE GS+ A LVE+A R R F S+Q +QG +L+ + G + Sbjct: 118 VILVFGRLLQGFSAGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIA 177 Query: 121 GLLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEFAAAERTETPLRDTF-SGQKM 179 + E++ WRL F GL+IGP G+YIRR L E+PEF +++ E+ D+F G K Sbjct: 178 VVYGQERMLQDYWRLAFFVGLVIGPVGWYIRRVLPETPEFERSKKKES--VDSFLEGVKA 235 Query: 180 ---RLLIGTGSVIMATVSVYLALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVASPLVGE 236 R+LIG +I TV+ ++ Y TYA+++L MP S+Y I SGL ++ PLVG Sbjct: 236 NFGRILIGIAIMIFWTVATQVSNYFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGM 295 Query: 237 LSDKYG-RTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPAL 295 L+D+ G P G++LA V+ YP+F L + P+ LLL+QI I V + +Y A P + Sbjct: 296 LADRIGPYKPMAFGAALAGVL-AYPLFKMLVATPTLPTLLLVQIAISVTLGLYAACAPLV 354 Query: 296 LADVFPVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQATGNKL 339 ++D+FP + R TG+ SY + VM+FGG +I+T LIQ +G+KL Sbjct: 355 MSDIFPTRYRATGIGTSYALGVMIFGGLTPMIVTTLIQISGDKL 398 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 244 bits (623), Expect = 9e-66, Method: Compositional matrix adjust. Identities = 140/360 (38%), Positives = 215/360 (59%), Gaps = 4/360 (1%) Query: 1 VTFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYSSIGLWA 60 +TF TFG F MRPLGA++LG+Y DRVGR+ L +++ +M IGT +IAF+P Y++IG+ A Sbjct: 56 LTFGTFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAA 115 Query: 61 PAGIVLARLIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGLSLLMAAVFGAGL 119 P ++ RL+QGFSAG E G + +L E A P +GF+ SWQ ASQ +++ +A+ G + Sbjct: 116 PILVLTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLV 175 Query: 120 NGLLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEFAAAERTET--PLRDTFSGQ 177 + L+P Q+ WGWR+PF G +I PA + IRR L E+ EF A +R + + + + Sbjct: 176 SQSLSPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQN 235 Query: 178 KMRLLIGTGSVIMATVSVYL-ALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVASPLVGE 236 ++ G V+M TVS YL +Y PT+ L + A S T GL P++G Sbjct: 236 WKIVVAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGA 295 Query: 237 LSDKYGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALL 296 LSD+ GR P ++ +L ++ YP+ +L S SF NLL+ ++ + L Y + L Sbjct: 296 LSDRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVAL 355 Query: 297 ADVFPVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQATGNKLSVSFFVIFGAALSFIAS 356 +V P R G SL+Y++A +FGGF L+ TWLI+ +G+K + +++ F A +A+ Sbjct: 356 TEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,179,226 Number of extensions: 53288 Number of successful extensions: 342 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 308 Number of HSP's successfully gapped: 17 Length of query: 366 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 280 Effective length of database: 1,212,660 Effective search space: 339544800 Effective search space used: 339544800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)