BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0122.0030.p (524 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 520 e-148 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 306 2e-84 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 235 6e-63 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 233 3e-62 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 200 2e-52 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 520 bits (1338), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/290 (85%), Positives = 270/290 (93%) Query: 235 RAAVDAGIPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRAT 294 RA DAG+P GV+NLVYGVP+EISEYLIPHP+IKKISFTGSTPVGKQLAALAG HMKRAT Sbjct: 188 RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRAT 247 Query: 295 MELGGHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAA 354 MELGGHAPA+VFDDAD+D AAK LA AKFRNAGQVCVSPTRFLVQK VF+AFVDKF+T A Sbjct: 248 MELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYA 307 Query: 355 SQLKVGDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVL 414 QLKVG+G AG TMGPVANKRRI ALEALI DAV QGAQLR GGKR+G+KGYFF+PTVL Sbjct: 308 EQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVL 367 Query: 415 TNVPLRARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRV 474 TN+PL+ARAMNEEPFGPLALINSF+TLDEVI EANRLP+GLAAYA+AKSA TKHAL++RV Sbjct: 368 TNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARV 427 Query: 475 ESGMITINHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQLDV 524 ESGM+TINHLGLALPE+PFGGIKDSGYGSEGGTEAIEAY+NTK V+QLDV Sbjct: 428 ESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLDV 477 Score = 400 bits (1027), Expect = e-112, Method: Compositional matrix adjust. Identities = 196/229 (85%), Positives = 208/229 (90%), Gaps = 1/229 (0%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRA+ADAG Sbjct: 135 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADAG 194 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 +P GV+NLVYGVP+EISEYLIPHP+IKKISFTGSTPVGKQLAALAG HMKRATMELGGHA Sbjct: 195 VPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELGGHA 254 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLKVGD 180 PA+VFDDAD+D AAK LA AKFRNAGQVCVSPTRFLVQK VF+AFVDKF+T A QLKVG+ Sbjct: 255 PAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYAEQLKVGN 314 Query: 181 GTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGVAR-GRQGLFLR 228 G AG TMGPVANKRRI ALEALI DAV QGAQLR G R G +G F + Sbjct: 315 GLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQ 363 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 306 bits (785), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 154/284 (54%), Positives = 205/284 (72%) Query: 239 DAGIPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELG 298 DAG+P GV N+V GV SEIS++LI P+++ ISFTGS PVG +LAA A HMKR +MELG Sbjct: 192 DAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKRMSMELG 251 Query: 299 GHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLK 358 GHAP +VFDDA+++ AA+ LA K RNAGQVCVSP+RF VQK ++ FV F A Q+K Sbjct: 252 GHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAEALLQIK 311 Query: 359 VGDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVP 418 VG G G MGP+ + RR A++ LI DA+ QG++L GG R+G +G+F+ PT++T VP Sbjct: 312 VGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAPTLITGVP 371 Query: 419 LRARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGM 478 AR M EEPFGP+A + F +E IA AN LP GLA+Y + +S T H +S +++GM Sbjct: 372 PSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVSRDLQAGM 431 Query: 479 ITINHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQL 522 + INH G+A PE+PFGGI +SG+GSEGG E E+++ TK +SQ+ Sbjct: 432 VNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQI 475 Score = 240 bits (612), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 122/227 (53%), Positives = 166/227 (73%), Gaps = 1/227 (0%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 +V++EPVG AA +PWNFP Q ++K++AA+AAGC++++K P E+PA+ + + F DAG Sbjct: 135 LVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVLMAQLFHDAG 194 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 +P GV N+V GV SEIS++LI P+++ ISFTGS PVG +LAA A HMKR +MELGGHA Sbjct: 195 VPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKRMSMELGGHA 254 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLKVGD 180 P +VFDDA+++ AA+ LA K RNAGQVCVSP+RF VQK ++ FV F A Q+KVG Sbjct: 255 PVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAEALLQIKVGP 314 Query: 181 GTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGVAR-GRQGLF 226 G G MGP+ + RR A++ LI DA+ QG++L G AR G++G F Sbjct: 315 GHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHF 361 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 235 bits (600), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 128/282 (45%), Positives = 176/282 (62%), Gaps = 2/282 (0%) Query: 240 AGIPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGG 299 AGIP GV ++V G P I + +PI++KISFTGST VGK L + + +K+ ++ELGG Sbjct: 200 AGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGG 259 Query: 300 HAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLKV 359 +AP IVFDDAD+DAA + +K+RNAGQ CV R VQ V+DAF K + A + KV Sbjct: 260 NAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKV 319 Query: 360 GDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVPL 419 G GT G T GP+ N++ + +E ++DAV +GA++ GGKR FFEPTVL +V Sbjct: 320 GQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTP 379 Query: 420 RARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGMI 479 + EE FGP+A + F T +EV+A AN FGLA+Y Y++ ++ +ESGM+ Sbjct: 380 AMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMV 439 Query: 480 TINHLGLALPEV-PFGGIKDSGYGSEGGTEAIEAYVNTKFVS 520 IN GL EV PFGG+K SG G EG I+ Y+ K++ Sbjct: 440 GIN-TGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 Score = 200 bits (508), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 103/220 (46%), Positives = 139/220 (63%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S L AG Sbjct: 142 VVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVLAERAG 201 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 IP GV ++V G P I + +PI++KISFTGST VGK L + + +K+ ++ELGG+A Sbjct: 202 IPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNA 261 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLKVGD 180 P IVFDDAD+DAA + +K+RNAGQ CV R VQ V+DAF K + A + KVG Sbjct: 262 PFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQ 321 Query: 181 GTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGVAR 220 GT G T GP+ N++ + +E ++DAV +GA++ G R Sbjct: 322 GTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKR 361 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 233 bits (594), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 133/294 (45%), Positives = 185/294 (62%), Gaps = 7/294 (2%) Query: 235 RAAVDAGIPDGVVNLVYGVPS---EISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMK 291 R A +AG+P GV+N+V E+ + + ++KISFTGST VGK LA + +K Sbjct: 197 RLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLK 256 Query: 292 RATMELGGHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFI 351 + ++ELGG+AP IVFDDAD+DAA + AKFRN GQ CVSP R VQ+ V+DAFVDK Sbjct: 257 KLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLG 316 Query: 352 TAASQLKVGDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGGKRVGDKGY---- 407 + LKVG T + +GP+ N R IA ++ + DA+ +GA++ GGKR+ G+ Sbjct: 317 ARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDN 376 Query: 408 FFEPTVLTNVPLRARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTK 467 ++ PTVL +V + EE FGP+A I F T DEVIA AN PFGLAAY Y+ + Sbjct: 377 YYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRI 436 Query: 468 HALSSRVESGMITINHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQ 521 H ++ +ESG++ +N LA PFGG+K+SGYG EG ++ Y++TK+V Q Sbjct: 437 HRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVCQ 490 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 105/223 (47%), Positives = 145/223 (65%), Gaps = 3/223 (1%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 M +KEPVG VAA TPWNFP + RK++ ALAAGC+++ K E+TP + L+R +AG Sbjct: 144 MALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVRLAQEAG 203 Query: 61 IPDGVVNLVYGVPS---EISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELG 117 +P GV+N+V E+ + + ++KISFTGST VGK LA + +K+ ++ELG Sbjct: 204 VPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLKKLSLELG 263 Query: 118 GHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLK 177 G+AP IVFDDAD+DAA + AKFRN GQ CVSP R VQ+ V+DAFVDK + LK Sbjct: 264 GNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLGARVAALK 323 Query: 178 VGDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGVAR 220 VG T + +GP+ N R IA ++ + DA+ +GA++ G R Sbjct: 324 VGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKR 366 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 200 bits (509), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 114/288 (39%), Positives = 158/288 (54%) Query: 235 RAAVDAGIPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRAT 294 R A +AG PDGV+N+V G + + L HP + KISFTGS G+ + +AG KR T Sbjct: 194 RLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVT 253 Query: 295 MELGGHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAA 354 +ELGG +P IVFDDA + A + F N GQVC + +R LVQ+++ F AA Sbjct: 254 LELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAA 313 Query: 355 SQLKVGDGTVAGTTMGPVANKRRIAALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVL 414 + + VGD + G MGPVA K + + I +++GA L GG DKG+F +PT+ Sbjct: 314 AGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIF 373 Query: 415 TNVPLRARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRV 474 + EE FGP+ I SFDT +E IA AN +GLAA + + H +++ V Sbjct: 374 ADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGV 433 Query: 475 ESGMITINHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQL 522 + G + +N +P+GGIKDSG G EGG AY K V+ L Sbjct: 434 KVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTVL 481 Score = 174 bits (441), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 94/228 (41%), Positives = 133/228 (58%), Gaps = 1/228 (0%) Query: 2 VIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAGI 61 ++EP+G V A PWN PL K++A LAAGC++++K EETP S L R +AG Sbjct: 142 TVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETPQSALHLARLAQEAGF 201 Query: 62 PDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHAP 121 PDGV+N+V G + + L HP + KISFTGS G+ + +AG KR T+ELGG +P Sbjct: 202 PDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSP 261 Query: 122 AIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFDAFVDKFITAASQLKVGDG 181 IVFDDA + A + F N GQVC + +R LVQ+++ F AA+ + VGD Sbjct: 262 QIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDP 321 Query: 182 TVAGTTMGPVANKRRIAALEALISDAVEQGAQLRF-GVARGRQGLFLR 228 + G MGPVA K + + I +++GA L GV+R +G F++ Sbjct: 322 SQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQ 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,520,337 Number of extensions: 64028 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 39 Length of query: 524 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 434 Effective length of database: 1,193,720 Effective search space: 518074480 Effective search space used: 518074480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)