BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0120.0004.p (504 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 811 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 136 5e-33 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 110 4e-25 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 102 7e-23 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 102 1e-22 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 811 bits (2096), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/518 (79%), Positives = 463/518 (89%), Gaps = 14/518 (2%) Query: 1 LPFRESLMATLGICFVIMLVALDQTIVGTALPTIVAELKGFDLYAWVATSFX-------- 52 LPFRESL+ATLGICFVIMLVALDQTIVGTALPTIVAELKGF+LYAWVAT++ Sbjct: 15 LPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAWVATAYLLTSVITVP 74 Query: 53 ------PFFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFASA 106 +FGRKPF+IASII+FV AS LCGMA+SMLFLVLARGLQGIGGGMLVGT FAS Sbjct: 75 IFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASV 134 Query: 107 ADLFPDPHIRLRWQIILSAAYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAM 166 ADLFPDP +RLRWQIILS ++GIAT+VGPSLGGFLTQ LGWRW FY NLP+G++SLFF Sbjct: 135 ADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVW 194 Query: 167 RYIPHIRHHKNAGKIRLDWIGAVLISVSLGSLQLLVELFPKRGLTGGMLALLVVAIGAFY 226 R++PHIRH ++AGK+RLDW GA+LIS+SLGSLQLLVEL PKRG+T GML LL V++ AFY Sbjct: 195 RFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSGMLVLLAVSVAAFY 254 Query: 227 ALWKWERRVEQPIVPFEMFKDPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMSPREA 286 ALWKWE+R QPI+PFEM +D AL+SLFLLSVLAGF+MFSMLMYAPLLFQGGFG+SPREA Sbjct: 255 ALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREA 314 Query: 287 GLLITPLVACITVASIVNGRIVTRIPNPNMMMYVGFVLIALSCLGIVLSQRDTPTGFMLA 346 GLLITPLVACITVASIVNGRI+TRIPNPN+MM +GF LIA SCLGIVL R T GF+LA Sbjct: 315 GLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLA 374 Query: 347 TMLAGGFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGMLGTAVTGTLVTQMYG 406 MLAGGFGLGLVLPNLTVFAQQAA+REHLGIATALLQSLRMIGGMLGTA+TGTLV+QMYG Sbjct: 375 VMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYG 434 Query: 407 SGVERALESDHAAQWLAEFKDPEILVNHDIQSTLLAQMLQAGHNGSALLEAAREALVGAI 466 +GVE+ALE DHAAQWL +F DPE+LVNHDIQS LLAQ++QAGH+GSALLEAAREALVGAI Sbjct: 435 AGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGSALLEAAREALVGAI 494 Query: 467 HMGIALAVVSALIGLWMVRRVPLIRFTPNEKVETVLSE 504 HMGIA+A+V+AL+GLWMVRRVP IRF ++KVE V+SE Sbjct: 495 HMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 532 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 136 bits (342), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 123/427 (28%), Positives = 197/427 (46%), Gaps = 31/427 (7%) Query: 8 MATLGICFVIMLVALDQTIVGTALPTIVAELKG-FDLYAWVATSF--------------X 52 + L I FV+ + LD T+V L I L W+ + Sbjct: 32 LVALAIGFVMAM--LDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALA 89 Query: 53 PFFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFASAADLFPD 112 FG + + + +FV+AS+LCG A + FL++AR LQG+G + ++ + ++ D Sbjct: 90 DRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYED 149 Query: 113 PHIRLRWQIILSAAYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHI 172 +R R + SA G+A +VGP +GG L LGWR F +N+PVGLV L A + IP + Sbjct: 150 DRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQV 209 Query: 173 RHHKNAGKIRLDWIGAVLISVSLGSLQLLVELFPKRGLTG-GMLALLVVAIGAFYALWKW 231 H A + G V+ L L ++ P G T +LA L VA+ A AL + Sbjct: 210 GGHARALNLSSHLAGVVM----LAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 232 ERRVEQPIVPFEMFKDPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMSPREAGLLIT 291 E R P++P +F P ++ + L F ++ L Y L Q G G + GL + Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 292 PLVACITVASIVNGRIVT----RIPNPNMMMYVGFVLIALSCLGIVLSQRDTPTGFMLAT 347 P++A I ++++GR+ R+P + + VG V AL+ +G TP + Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPL-LLGLSVGTVFTALA-MGF---SPATPYWLLALV 380 Query: 348 MLAGGFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGMLGTAVTGTLVTQMYGS 407 + G+ +P +T Q A + H A A L + R IG ++G A+ G ++ + G Sbjct: 381 CASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGW 440 Query: 408 GVERALE 414 + L Sbjct: 441 NLRNPLA 447 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 110 bits (274), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 114/437 (26%), Positives = 190/437 (43%), Gaps = 35/437 (8%) Query: 1 LPFRESLMATLGICFVIMLVALDQTIVGTALPTIVAELK-GFDLYAWVATSF-------- 51 LP ++ A L + + D TI+ ALP I L+ + WV+ ++ Sbjct: 22 LPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAMTL 81 Query: 52 ------XPFFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFAS 105 G + A + +F +AS+ C ++ S+ L+L R LQG+GG ++ A A Sbjct: 82 AAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAPAI 141 Query: 106 AADLFPDPHIRLRWQIILSAAYGIA--TSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLF 163 +FP+ RL +I+ A +A T+VGP LGG L LGW W F +N+P+G+++++ Sbjct: 142 HRTIFPN---RLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVW 198 Query: 164 FAMRYIPHIRHHKNAGKIRLDWIGAVLISVSLGSLQLLVEL---FPKRGLTGGMLALLVV 220 A +P R A D GA+ ++++G+L + + F + G G Sbjct: 199 LAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAA 255 Query: 221 AIGAFY----ALWKWERRVEQPIVPFEMFKDPALSSLFLLSVLAGFSMFSMLMYA-PLLF 275 A + +RR P++P ++F +P SL L+ LA F + A P LF Sbjct: 256 YAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLASFVGQGIAFVALPFLF 314 Query: 276 QGGFGMSPREAGLLITPLVACITVASIVNGRIVTRIPNPNMMMYVGFVLIALSCLGIVLS 335 Q +G + E+ L TP I + + GR+ R + + L +L Sbjct: 315 QNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLP 374 Query: 336 QRDTPTGFMLATMLAG-GFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGMLGT 394 + P ++ G GFG N + SRE G A+ +L +R G LG Sbjct: 375 DQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNASGVLAIMRTFGQCLGA 432 Query: 395 AVTGTLVTQMYGSGVER 411 A+ G ++ + V + Sbjct: 433 ALLGVILAVRTAAAVAQ 449 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 102 bits (254), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 111/435 (25%), Positives = 180/435 (41%), Gaps = 33/435 (7%) Query: 22 LDQTIVGTALPTIVAELKGFDL--------YAWVATSFXPF-------FGRKPFLIASII 66 LD TIV TALP + L L Y S P FG + A+I+ Sbjct: 67 LDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAIL 126 Query: 67 IFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFASAADLFPDPHIRLRWQIILSAA 126 +F + S+LC MA S+ L+ AR +QG+GG ML+ + P I S A Sbjct: 127 LFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASI-SIA 185 Query: 127 YGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHIRHHKNAGKIRLDWI 186 + VGP LGG+L +SL W W F +NLPVG++ + + ++P AG R D + Sbjct: 186 GQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLP---DDSLAGTPRFDLL 242 Query: 187 GAVLISVSLGSLQLLVELFPKRGLTGGMLALLVVAIGAFYALWKWERRVEQPIVPFEMFK 246 G VL+S + + L +++ TG +AL VVA W+ +P+ +F Sbjct: 243 GCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVLGYWRHASVHPRPLFRLGLFG 302 Query: 247 DPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMSPREAGLLITPLVACITVASIVNGR 306 +P S +++ ++ PLL Q G SP AG+++ P T+A Sbjct: 303 EPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAP 362 Query: 307 IVTRIPNPNMMMYVGFVLIALSCLGIVLSQRDTPTGFMLATMLAGGFGLGLVLPNLTVFA 366 +V R + + V ++ + + L D P + + G + + Sbjct: 363 LVKRF-GYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVT 421 Query: 367 QQAASREHLGIATALLQSLRMIGGMLGTAVTGTLVTQMYGSGVERALESDH--------- 417 + + G +L ++M+ LG + GT+V ++ S V A + Sbjct: 422 LKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMV-NLFKSEVGTATNASFQITFAIVGA 480 Query: 418 ---AAQWLAEFKDPE 429 A+ W+ DP Sbjct: 481 ITLASAWVFRKMDPR 495 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 102 bits (253), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 116/404 (28%), Positives = 182/404 (45%), Gaps = 43/404 (10%) Query: 22 LDQTIVGTALPTIVAELK------GFDLYAWVAT--SFXPFFG--------RKPFLIASI 65 +D T++ T+LP + +L + A+VA F P G RK F A++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVF-SAAM 59 Query: 66 IIFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTA---FASAADLFPDPHIRLRWQII 122 I+F+ S+LC ++ S++ V AR LQGIGG M+V A + D ++ + Sbjct: 60 IVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVD--KSELVKAVSYLT 117 Query: 123 LSAAYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHIRHHKNAGKIR 182 L A G VGP LGGF+T WRW F +N+P + L+ A R+I + + A Sbjct: 118 LPALLG--PVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPA---P 172 Query: 183 LDWIGAVLISVSLGSLQLLVELFPKRGLTGGMLALLVVAIG--AFYALWKWERRVEQPIV 240 LD G VL SV + L + LF L G + + +IG A ++ ++E P++ Sbjct: 173 LDVKGFVLTSVGAAMIMLGLSLFGSH-LVDGATPVAMCSIGAIALVMYYRHSTQIEFPLL 231 Query: 241 PFEMFKDPALSSLFLLSVLAGFSMFSMLMYA-----PLLFQGGFGMSPREAGLLITPLVA 295 F + + P L + SV+ G S+F + + A PL Q G GM+ +AG + Sbjct: 232 DFRLLRIPTLHA----SVVGG-SLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAF 286 Query: 296 CITVASIVNGRIVTRIPNPNMMMYVG-FVLIALSCLGIVLSQRDTPTGFMLATMLAGGFG 354 ++ GR++ R ++M F IAL+ GI TP +++ +L GG Sbjct: 287 GSLFIRLLAGRVLRRYGFRTVLMVNAVFAGIALAAYGIF--SIHTPYLLIVSIVLMGGVF 344 Query: 355 LGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGMLGTAVTG 398 L L A G T+L ++ + LG V G Sbjct: 345 PALQFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAG 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,497,741 Number of extensions: 61065 Number of successful extensions: 423 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 396 Number of HSP's successfully gapped: 13 Length of query: 504 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 415 Effective length of database: 1,198,455 Effective search space: 497358825 Effective search space used: 497358825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)