BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0117.0020.p (239 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 194 4e-51 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 183 1e-47 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 64 2e-11 gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [... 56 3e-09 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 194 bits (494), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 106/231 (45%), Positives = 144/231 (62%), Gaps = 13/231 (5%) Query: 17 ACASTPYQGDSYTVRKGDTLMSISRTVGKDHHDIAAWNKLSDANEIYPGDVLRLKPPATV 76 CAST YTV++GDTL SI R + ++ WN+L + +++ G + ++PP V Sbjct: 23 GCASTADSDGYYTVKRGDTLYSIGRDFDQSPANLRTWNRLRNPDDLEVGQRILVRPPVGV 82 Query: 77 AAAPVSRAKPAAATTAARESKAESRKAPESATPAA------------SAAEAERLDWMWP 124 A + P A +++ + + P + P A + AEA ++DW WP Sbjct: 83 VARTGAGQSPRPAASSSPSASGSGSRKPAPSRPTAKPAAKPQDDDAPAGAEA-KVDWSWP 141 Query: 125 AQGKLTATTDRNLKGIDIAGTPGQPVWAAESGKVTYAGRGIRGYGNMIIVKHSRTLLSVY 184 GK + D KGIDI G+ GQ V AA +GKVTYAG GIRGYGNM+I+KH+ LLSVY Sbjct: 142 TAGKSAPSADSYKKGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVY 201 Query: 185 AHNKSIMVKEGQSVNRGQQIAEMGDTDSRTVKLYFEIRSNGKPLNPVPLLP 235 AHNK+I+VKEGQ+V RGQQIA MG++D+ VKLYFEIR NGKP+N + +LP Sbjct: 202 AHNKTILVKEGQTVTRGQQIATMGNSDTNKVKLYFEIRRNGKPINVMAVLP 252 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 183 bits (464), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 125/322 (38%), Positives = 157/322 (48%), Gaps = 95/322 (29%) Query: 11 VVVALAACASTPYQGDS-----------------------------YTVRKGDTLMSISR 41 VV LAAC STP Q + YTV+KGDTL I+ Sbjct: 12 VVSLLAACGSTPNQQPAPVVERTSSTGKVVDAATAPAAPAPSGRGYYTVKKGDTLYRIAL 71 Query: 42 TVGKDHHDIAAWNKLSDANEIYPGDVLRLKP----------------------------P 73 G++ DI AWN L++ N+I LR+ P P Sbjct: 72 EAGQNWRDIVAWNNLTNPNDIKVDQTLRVAPPDRAPGTAQTAAIAASPAAGLEVKPLGAP 131 Query: 74 ATVA-AAPV----SRAKPAAATTAARESK-AESRKAPESATPAASA-------------- 113 T A AAPV ++ P ES AE K + PAAS Sbjct: 132 GTSAPAAPVPVGSNKTGPRGDKRPYSESALAELEKPDAPSAPAASGTSVASAAPSAPAKA 191 Query: 114 -----------------AEAERLDWMWPAQGKLTATTDRNLKGIDIAGTPGQPVWAAESG 156 A+ E + WMWPA+GK+ T + KG+DIAG GQ V AA +G Sbjct: 192 EQPHAADKPAAAASQAAADDEGVSWMWPAEGKVVGTFEGGKKGVDIAGKAGQAVLAAGAG 251 Query: 157 KVTYAGRGIRGYGNMIIVKHSRTLLSVYAHNKSIMVKEGQSVNRGQQIAEMGDTDSRTVK 216 KV YAG GIRGYGN++I+KH+ LLS YAHN++I+VKEGQSV +GQ+IAEMG +DS TVK Sbjct: 252 KVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKSDSDTVK 311 Query: 217 LYFEIRSNGKPLNPVPLLPKPR 238 L+FEIR GKP++P LP PR Sbjct: 312 LHFEIRQQGKPVDPSKYLP-PR 332 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 32/94 (34%), Positives = 54/94 (57%), Gaps = 2/94 (2%) Query: 138 KGIDIAGTPGQPVWAAESGKVTYAGRGIRGYGNMIIVKHSRTLLSVYAHNKSIMVKEGQS 197 +G+ I G V A +G+V +A +RG+GN+IIV H +++Y +N+S++ + G Sbjct: 371 RGLFIRAHEGAEVRAVAAGRVVFAD-WLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDL 429 Query: 198 VNRGQQIAEMGDT-DSRTVKLYFEIRSNGKPLNP 230 V G IA G++ + LYFE+R G+ +P Sbjct: 430 VKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDP 463 >gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 55.8 bits (133), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 32/96 (33%), Positives = 50/96 (52%), Gaps = 4/96 (4%) Query: 139 GIDIAGTPGQPVWAAESGKVTYAGRGIRGYGNMIIVKHSRTLLSVYAHNKSIM--VKEGQ 196 G+D A G P+ AA G V + G+ GYGN++++KH + Y H +K+G Sbjct: 366 GVDFAAPTGTPIHAAADGVVDFVGKQ-NGYGNIVVIKHWSGYSTAYGHMSRFAPDIKKGM 424 Query: 197 SVNRGQQIAEMGDTDSRTV-KLYFEIRSNGKPLNPV 231 V++ I +G T T L++E R N +P NP+ Sbjct: 425 KVSQNDVIGFVGMTGWATGPHLHYEFRVNNQPRNPL 460 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.128 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 581,693 Number of extensions: 20093 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 69 Number of HSP's successfully gapped: 6 Length of query: 239 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 157 Effective length of database: 1,231,600 Effective search space: 193361200 Effective search space used: 193361200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)