BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0009.p (340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 483 e-137 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 358 e-100 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 271 5e-74 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 182 3e-47 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 132 4e-32 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 483 bits (1242), Expect = e-137, Method: Compositional matrix adjust. Identities = 241/285 (84%), Positives = 262/285 (91%) Query: 22 MDSTVISTSLPVLAIDIAVDPISLKLALTSYLVSLAVFIPISGWMADRFGALTIFRSAIC 81 MDSTVISTSLP LA+D+ VDPISLKLALTSYLVSLAVFIPISGWMADRFG+LTIFRSAIC Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 82 VFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLVKALSYLTIPA 141 VFMLGSILCGVS +LP+FVAARF QGIGGAMMVPVGRLVILRSV+K NLVKALSYLTIPA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 142 MFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANARAESVAPLDWVGFLL 201 +FGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFI + +AE VAPLD GFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 202 SALGCSLVMMGLATAGRHLVSGDISLVCVVLGSLSLVAYVWHARRTEHPLIDLKLLKIET 261 SALGCSL+MMGLATAGRHLVSG+++L CV+ G + L AYVWHARR PLIDL LLKI T Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 262 LQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASA 306 L+AGV GGS +R+GIGAIPFLLPLMLQLGFG++ FHSG+LTCASA Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASA 285 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 358 bits (919), Expect = e-100, Method: Compositional matrix adjust. Identities = 175/285 (61%), Positives = 224/285 (78%) Query: 22 MDSTVISTSLPVLAIDIAVDPISLKLALTSYLVSLAVFIPISGWMADRFGALTIFRSAIC 81 MD+TV++TSLP LA D+ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ GA +F +A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 82 VFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLVKALSYLTIPA 141 VFM GSILC VS SL FVAARF QGIGGAMMVPVGR++I RSVDK+ LVKA+SYLT+PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 142 MFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANARAESVAPLDWVGFLL 201 + GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI N + ++ APLD GF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 202 SALGCSLVMMGLATAGRHLVSGDISLVCVVLGSLSLVAYVWHARRTEHPLIDLKLLKIET 261 +++G +++M+GL+ G HLV G + +G+++LV Y H+ + E PL+D +LL+I T Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 262 LQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASA 306 L A V GGSLFR+G+GA+PFLLPL LQ G G +AF +G +TCASA Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASA 285 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 271 bits (693), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 138/294 (46%), Positives = 189/294 (64%) Query: 5 RTFTIPLIVACALFMEQMDSTVISTSLPVLAIDIAVDPISLKLALTSYLVSLAVFIPISG 64 R +P IVA FME +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISG Sbjct: 28 RVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISG 87 Query: 65 WMADRFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRS 124 W+ADRFG+ T+F AI +F L SILCG SQ++ EF AAR QG+GGAMMVPVGR++++RS Sbjct: 88 WVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRS 147 Query: 125 VDKANLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIA 184 K L+KA++ +T PA+ PV+GP +GGFITTY W WIF +N+P + + Sbjct: 148 TGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVR 207 Query: 185 NARAESVAPLDWVGFLLSALGCSLVMMGLATAGRHLVSGDISLVCVVLGSLSLVAYVWHA 244 N S PLD VGFLLS + ++ G A + ++L V LG + H Sbjct: 208 NEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHM 267 Query: 245 RRTEHPLIDLKLLKIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHS 298 +R EHPL+D L+I T V GSL R+G+ A+P+L+PLM Q+GFG +AF S Sbjct: 268 QRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQS 321 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 182 bits (462), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 108/298 (36%), Positives = 173/298 (58%), Gaps = 7/298 (2%) Query: 12 IVACALFMEQMDSTVISTSLPVLAIDIAVDPISLKLALTSYLVSLAVFIPISGWMADRFG 71 IVA FM+ +D+T+++T+LP +A + +++ + +Y +++A P SGW+ADRFG Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 72 ALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLV 131 ++ +AI +F LGS+LC ++ SLP +AAR QG+GG+M++PVGRL +LR+V A + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 132 KALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANARAESV 191 AL+ ++I GPV+GP LGG++ W WIF IN+P+ +LG+ F+ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 192 APLDWVGFLLSALGCSLVMMGLATA---GRHLVSGDISLVCVVLGSLSLVAYVWHARRTE 248 D +G +L L +V LA H I+L V+ +L+++ Y HA Sbjct: 237 PRFDLLGCVL--LSACMVAFSLALDVPFDSHRTGWSIAL--FVVAALTVLGYWRHASVHP 292 Query: 249 HPLIDLKLLKIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASA 306 PL L L G+ G + R+G A+PFLLPL+LQ+ G+S H+G++ +A Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAA 350 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 132 bits (332), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 95/306 (31%), Positives = 165/306 (53%), Gaps = 24/306 (7%) Query: 11 LIVACALFMEQMDSTVISTSLPVLAIDIAVDPISLKLALTSYLVSLAVFIPISGWMADRF 70 L V+ A+FM +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 71 GALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANL 130 GA+ +F +++ +F+L S LCG++ +LP +AAR QG+ MVP+ + ++L + K+ Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 131 VKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLATRFIANARA 188 AL+ + A+ PV GP LGG+IT + W WIF+INIP+ ++ G+ LA + + Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAI-YRSRETP 205 Query: 189 ESVAPLDWVG--FLLSALGCSLVMMGLATAGRHLVSGDISLVCVVLGSLSLVAY----VW 242 P+D +G L++ + +M+ G+ L S V LG ++L+A+ VW Sbjct: 206 THKLPIDKIGLALLVTWVAAFQIML---DKGKDL-DWFASPTIVTLGIIALIAFAYFVVW 261 Query: 243 HARRTEHPLIDLKLLKIETLQAG-----VGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFH 297 +HP++DL+L G +G G F G + +LP LQ G+ + Sbjct: 262 ELNN-DHPVVDLRLFGRRNFAGGTIAISIGYGVFF----GNL-VILPQWLQQYLGYRSID 315 Query: 298 SGLLTC 303 SGL T Sbjct: 316 SGLSTA 321 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 869,142 Number of extensions: 33048 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 16 Length of query: 340 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 254 Effective length of database: 1,212,660 Effective search space: 308015640 Effective search space used: 308015640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)