BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0006.p (560 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 791 0.0 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 514 e-146 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 499 e-142 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 249 5e-67 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 241 9e-65 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 791 bits (2042), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/539 (74%), Positives = 454/539 (84%), Gaps = 49/539 (9%) Query: 1 MNISRPFIERPIATTLLTIGIALAGMLAFKLLPVSPLPQVDYPTISVSASMPGASPETMA 60 MNIS PFI+RPIATTLLTIGIALAG++AF+LLPVSPLPQVDYPTIS+SASMPGASPETMA Sbjct: 1 MNISLPFIQRPIATTLLTIGIALAGIVAFRLLPVSPLPQVDYPTISISASMPGASPETMA 60 Query: 61 ATIATPLDAE-----------------------QSDLPQGES------------------ 79 AT+ATPL+ Q DL + Sbjct: 61 ATVATPLERAMGAIAGVTEMTSTSSQNSTRITLQFDLSRDIDGAARDVQAALNAARNLLP 120 Query: 80 XGMPSSPTYRKVNPADAPIMILALTSDTMTQGQMYDAADSILAQKLSQVRGIGQVSVGGS 139 GMPS+PTYRKVNPADAPI+ILA+TSDTMTQGQMYDAADSI+AQKLSQVRG+GQVSVGGS Sbjct: 121 TGMPSNPTYRKVNPADAPILILAMTSDTMTQGQMYDAADSIVAQKLSQVRGVGQVSVGGS 180 Query: 140 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGIVEDGDRNWQIYANDQVKTAAE 199 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKG++ED DRNWQIYANDQ KTAAE Sbjct: 181 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGLLEDQDRNWQIYANDQAKTAAE 240 Query: 200 YAPLIVSYRNGAPVRVRDIAEVRDSVADLRNSGSANGKPSVLVILNRQPGANIIETVDAV 259 YAPLIVSYRNG PVRV+D+AEVRDSVAD+RN+GS+NGKPSVL+I+NRQPGANII+TVD V Sbjct: 241 YAPLIVSYRNGTPVRVQDVAEVRDSVADVRNAGSSNGKPSVLIIINRQPGANIIDTVDNV 300 Query: 260 DALLPNLKASIPNAINLEVMMDRTPTIRASLKDTEKTLLMSIALVIMVVFLFLRNGRATL 319 LLP L+ASIP+AI+++V++DRTPTIRASLK+TE TL++S+ALVIMVVFLFLRN RATL Sbjct: 301 RELLPQLQASIPSAIDMKVVLDRTPTIRASLKETEFTLILSVALVIMVVFLFLRNARATL 360 Query: 320 IPSVAVPVSLIGTFGVMYLLGYTLDNLSLMALTIATGFVVDDAIVVLENISRHIEQGMKP 379 IP+VAVPVSLIGTFGVMYL G++LDNLSLMALTIATGFVVDDAIVVLENISRHIE GMKP Sbjct: 361 IPAVAVPVSLIGTFGVMYLAGFSLDNLSLMALTIATGFVVDDAIVVLENISRHIESGMKP 420 Query: 380 FRAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGRLFREFAVTLSVAILVSLVVSLT 439 ++AAL+GAREVGFTVLSMSISLIAVFIPILMM GIVGRLFREFA+TLSVAILVSLVVSLT Sbjct: 421 YKAALLGAREVGFTVLSMSISLIAVFIPILMMEGIVGRLFREFAITLSVAILVSLVVSLT 480 Query: 440 TTPMMCAKLLKHQ--SEEERKPGR------FFSAVERLFDSMLRGYERSLAWALRFSPL 490 TTPMMCA+LL+H ++E R+ R +F+ ML Y+R+LAWALR +PL Sbjct: 481 TTPMMCAQLLRHDPAADEARRQRRHPWLQKIADQSAAIFEDMLLAYQRTLAWALRAAPL 539 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 514 bits (1323), Expect = e-146, Method: Compositional matrix adjust. Identities = 261/526 (49%), Positives = 363/526 (69%), Gaps = 43/526 (8%) Query: 1 MNISRPFIERPIATTLLTIGIALAGMLAFKLLPVSPLPQVDYPTISVSASMPGASPETMA 60 MN SR FI RP+AT+LL + I L G++A++LLP+S LP+VDYPTI V PGASP+ M Sbjct: 1 MNPSRQFILRPVATSLLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 Query: 61 ATIATPLDAEQSDLP-----QGESXG---------------------------------- 81 +++ PL+ + +P S G Sbjct: 61 SSVTAPLERQFGQMPGLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLP 120 Query: 82 --MPSSPTYRKVNPADAPIMILALTSDTMTQGQMYDAADSILAQKLSQVRGIGQVSVGGS 139 +P P Y KVNPAD PI+ LA+TS+TM ++ D D+ +AQK+SQ+ G+G VS+ G Sbjct: 121 SDLPMPPVYSKVNPADTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGG 180 Query: 140 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGIVEDGDRNWQIYANDQVKTAAE 199 +PAVR++LNP A+ G+ DVRTA+ N N+ KG + R I ANDQ+++A E Sbjct: 181 QRPAVRMQLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADE 240 Query: 200 YAPLIVSYRNGAPVRVRDIAEVRDSVADLRNSGSANGKPSVLVILNRQPGANIIETVDAV 259 Y LI++Y+NGAP+RV D+A+V D ++R AN +P+V+V + RQPGAN+I VD++ Sbjct: 241 YRNLIIAYKNGAPIRVSDVADVVDGAENVRLGAWANTQPAVIVNIQRQPGANVIAVVDSI 300 Query: 260 DALLPNLKASIPNAINLEVMMDRTPTIRASLKDTEKTLLMSIALVIMVVFLFLRNGRATL 319 +LP L+AS+P AI++ ++ DRT TIRAS+ D + LL+S+ALV+MV+F+FLRN AT+ Sbjct: 301 KKILPQLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIFVFLRNVPATI 360 Query: 320 IPSVAVPVSLIGTFGVMYLLGYTLDNLSLMALTIATGFVVDDAIVVLENISRHIEQGMKP 379 IPSVAVP+SL+GTF +MYL G++++NL+LMALTIATGFVVDDAIV++ENISR+IE+GMKP Sbjct: 361 IPSVAVPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENISRYIEEGMKP 420 Query: 380 FRAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGRLFREFAVTLSVAILVSLVVSLT 439 +AAL GA ++GFT++S++ SLIAV IP+L MG +VGRLF EFAVTL+V+IL+S VVSLT Sbjct: 421 MQAALKGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEFAVTLAVSILISAVVSLT 480 Query: 440 TTPMMCAKLLKHQSEEERKPGRFFSAVERLFDSMLRGYERSLAWAL 485 TPMMCA+LL+HQ EE++ G F+ ++ FD ++ Y L W L Sbjct: 481 LTPMMCARLLRHQPEEQQ--GWFYHKSQQFFDRIIARYGVMLQWVL 524 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 499 bits (1286), Expect = e-142, Method: Compositional matrix adjust. Identities = 257/535 (48%), Positives = 366/535 (68%), Gaps = 57/535 (10%) Query: 1 MNISRPFIERPIATTLLTIGIALAGMLAFKLLPVSPLPQVDYPTISVSASMPGASPETMA 60 M++S FI+RPI TTLL I I L G+ + LLPV+PLPQVD+PTI V+A +PG SPETMA Sbjct: 1 MSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 Query: 61 ATIATPLDAE-----------------------QSDLPQG-------------ESXG--- 81 + +A PL+ + Q DL + S G Sbjct: 61 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP 120 Query: 82 --MPSSPTYRKVNPADAPIMILALTSDTMTQGQMYDAADSILAQKLSQVRGIGQVSVGGS 139 +P+ PT+RK+NPAD+PIMI+++ S+ + + D AD+ILAQ++SQ+ G+G V+V G Sbjct: 121 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ 180 Query: 140 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGIVEDGDRNWQIYANDQVKTAAE 199 +PAVRI+++P L G+ D+R +A T N+PKG V+ + + +Y NDQ+ A + Sbjct: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 Query: 200 YAPLIVSYRNGAPVRVRDIAEVRDS-------------VADLRNSGSANGKPSVLVILNR 246 + +++++RNGAP+RV+DI D A+ + NG+ VL I + Sbjct: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAI-TK 299 Query: 247 QPGANIIETVDAVDALLPNLKASIPNAINLEVMMDRTPTIRASLKDTEKTLLMSIALVIM 306 QPGAN+IETV + A +P L+A+IP AI++ ++DRT TIRAS++D E TL+++IALV+M Sbjct: 300 QPGANVIETVGRIKAAMPRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVLTIALVVM 359 Query: 307 VVFLFLRNGRATLIPSVAVPVSLIGTFGVMYLLGYTLDNLSLMALTIATGFVVDDAIVVL 366 V+F+FLRN ATLIPSV VP++++GT GVMY++GY+LDNLSLMALTIA GFVVDDAIV+L Sbjct: 360 VIFVFLRNVAATLIPSVTVPLAIMGTAGVMYVVGYSLDNLSLMALTIAVGFVVDDAIVML 419 Query: 367 ENISRHIEQGMKPFRAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGRLFREFAVTL 426 ENI R++E+GM P AAL GA E+GFT++S+S+SL+AVFIP+L+MGGIVGRLFREFAVT+ Sbjct: 420 ENIYRYVEEGMTPLEAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIVGRLFREFAVTV 479 Query: 427 SVAILVSLVVSLTTTPMMCAKLLKHQSEEERKPGRFFSAVERLFDSMLRGYERSL 481 ++ I VS+++SLT TPM+C++ LK+ E ++ G+ + ER FD+ML GY+R L Sbjct: 480 TLTIAVSVIISLTLTPMLCSRFLKN--EHGQRHGKLYQWFERCFDAMLAGYKRGL 532 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 249 bits (635), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 164/537 (30%), Positives = 286/537 (53%), Gaps = 54/537 (10%) Query: 3 ISRPFIERPIATTLLTIGIALAGMLAFKLLPVSPLPQVDYPTISVSASMPGASPETMAAT 62 ++R FI+RPI ++ I I L GM++ + L + P + P +++ A+ GAS +T+ + Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 63 IATPLDAEQSDLP--------------------------------------QGESXGMPS 84 + ++ + L Q + +P Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 Query: 85 SPTYRKVNPADAP---IMILALTSDT--MTQGQMYDAADSILAQKLSQVRGIGQVSVGGS 139 + V A +M+L+LTSD +T + D S L ++S+V G+G V+ G+ Sbjct: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 Query: 140 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGIV-----EDGDR-NWQIYANDQ 193 + A+RI ++P L KY + +D+ +A+ A N G + + G + N I A + Sbjct: 181 NY-AMRIWIDPAKLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITARSK 239 Query: 194 VKTAAEYAPLIV-SYRNGAPVRVRDIAEVRDSVADLRNSGSANGKPSVLVILNRQPGANI 252 + T E+ +IV S +G+ V++ D+A V + + NGKPS + + GAN Sbjct: 240 LTTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANA 299 Query: 253 IETVDAVDALLPNLKASIPNAINLE--VMMDRTPTIRASLKDTEKTLLMSIALVIMVVFL 310 + T DAV L L+ P ++ L+ + D TP +R SL++ KTL+ +I LV+++++L Sbjct: 300 LATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYL 359 Query: 311 FLRNGRATLIPSVAVPVSLIGTFGVMYLLGYTLDNLSLMALTIATGFVVDDAIVVLENIS 370 FL+N RAT IP++ VPV L+GTFG++ + GY+++ L++ + +A G +VDDAIVV+EN+ Sbjct: 360 FLQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVDDAIVVVENVE 419 Query: 371 RHI-EQGMKPFRAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGRLFREFAVTLSVA 429 R + E+ + P A +E+ ++ +++ L AVFIP+ GG G ++R+F++T+ A Sbjct: 420 RLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQFSITIVSA 479 Query: 430 ILVSLVVSLTTTPMMCAKLLKHQSEEERKPGRFFSAVERLFDSMLRGYERSLAWALR 486 + +S++V+LT TP +CA LLK + + GRFF+ R FD Y+ +A L+ Sbjct: 480 MALSVMVALTLTPALCATLLKPHAGGHVQQGRFFTWFNRSFDRSATRYQGGVAGLLK 536 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 241 bits (616), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 162/521 (31%), Positives = 286/521 (54%), Gaps = 53/521 (10%) Query: 3 ISRPFIERPIATTLLTIGIALAGMLAFKLLPVSPLPQVDYPTISVSASMPGASPETMAAT 62 ++R FI+RPI +L I + LAG+++ LP++ P + P I+++A+ PGAS +T+ T Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 63 IATPLDAEQSDLPQ-------GESXGMPS-SPTYRK-VNPADAPIMI------------- 100 + ++ + L + ES G + + T+ NP A + + Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPS 120 Query: 101 -------------------LALTSD--TMTQGQMYDAADSILAQKLSQVRGIGQVSVGGS 139 LA TS+ +M+ + D + + +S+V G+G ++ GS Sbjct: 121 EVQQQGITVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFGS 180 Query: 140 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGIV------EDGDRNWQIYANDQ 193 Q A+RI L+P LN YG+ DV+ A+ A NA G + E+ N I A + Sbjct: 181 -QYAMRIWLDPNKLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQQINATITAQTR 239 Query: 194 VKTAAEYAPLIV-SYRNGAPVRVRDIAEVRDSVADLRNSGSANGKPSVLVILNRQPGANI 252 ++TA ++ +++ + GA V++RD+A + + G NGKP+ + + GAN Sbjct: 240 LRTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAGLAIKLATGANA 299 Query: 253 IETVDAVDALLPNLKASIPNAINLEVMMDRTPTIRASLKDTEKTLLMSIALVIMVVFLFL 312 ++TV A+D + L+ P + ++ D TP +R S+++ +TL+ ++ LV +V++LFL Sbjct: 300 LDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAVVLVFLVMYLFL 359 Query: 313 RNGRATLIPSVAVPVSLIGTFGVMYLLGYTLDNLSLMALTIATGFVVDDAIVVLENISRH 372 +N RATLIP++AVPV L+GTFGV+ + G+T++ L++ A+ +A G +VDDAIVV+EN+ R Sbjct: 360 QNFRATLIPTIAVPVVLLGTFGVLAVFGFTINTLTMFAMVLAIGLLVDDAIVVVENVERV 419 Query: 373 I-EQGMKPFRAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGRLFREFAVTLSVAIL 431 + E+G+ P +A ++ ++ +++ L AVF+P+ G G ++R+F++T+ A+ Sbjct: 420 MSEEGLPPKQATRKSMGQISGALVGVALVLAAVFVPMAFFSGSTGVIYRQFSITIVSAMT 479 Query: 432 VSLVVSLTTTPMMCAKLLKHQSE-EERKPGRFFSAVERLFD 471 +S++V+L TP +CA LLK ++ FF R FD Sbjct: 480 LSVLVALVLTPALCATLLKPAAKGHALATTGFFGWFNRQFD 520 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,529,567 Number of extensions: 58882 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 264 Number of HSP's successfully gapped: 17 Length of query: 560 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 470 Effective length of database: 1,193,720 Effective search space: 561048400 Effective search space used: 561048400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)