BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0015.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 645 0.0 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 102 7e-23 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 100 1e-22 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 77 3e-15 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 645 bits (1665), Expect = 0.0, Method: Compositional matrix adjust. Identities = 301/396 (76%), Positives = 354/396 (89%), Gaps = 3/396 (0%) Query: 1 LRGRILATVHNQLDAYLSACQLGITLASLGLGWIGEPAFASLLEPLFALLGVSSLELIHG 60 +RGRILA VHNQLDAYLSACQLGITLASLGLGWIGEPAFA +LEPLF+L GV++ ELIHG Sbjct: 44 MRGRILAVVHNQLDAYLSACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHG 103 Query: 61 VSFVFAFVIISFLHIVVGELAPKSMAIRNPERIGLWCAMPLYGFYWMMYPAIWLLNLSAN 120 VSFVFAF +ISFLHIV GELAPKSMAIR+PE++GLWCAMPLYGFYW MYP IW+LN S+N Sbjct: 104 VSFVFAFFVISFLHIVAGELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSN 163 Query: 121 WVLRVTGLGGVHGHDSHYSSDELKLILRSG---GKTGKFTPQEWSVLAQSLDFGDLKVMD 177 W+LRV GLG HGHD+HYSSDELKLILR+G GK GKFT EW+VL QSL+F +L V D Sbjct: 164 WLLRVAGLGAGHGHDAHYSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVAD 223 Query: 178 LMRPANEIVALSAANTHDENMQIIYRNRFSRYPYFDAGQQEVLGLLHLKDVFIAQQDGKA 237 +MRPA+EIVAL + +EN+ IIYRNR+SRYPY+DA +Q+VLGL+HLKDVF+AQQDG+A Sbjct: 224 IMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRA 283 Query: 238 IVDLRHYLRPVQYISPSLSAQELLRRFRTGSPHFAIIGRKGQAPLGFVTLDNLLSVLVGE 297 I +L+ YLRPVQYISP+L A +LLRRFRTGSPHFA+IG+KGQ P GF+TLDN+LS+LVGE Sbjct: 284 IANLKDYLRPVQYISPALPALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGE 343 Query: 298 IRDEFRHTSNEWTRQDDGTLLGKGSLPIVTLEHMLGIDFEYDETVDSVGGLVMEKLGEVP 357 IRDEFRH + EWTRQDDGTLLGKGSLPIVTLE+MLGID EYD+++DSVGGLVMEKLG++P Sbjct: 344 IRDEFRHNTGEWTRQDDGTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLP 403 Query: 358 KEGQKVEFHAFDIVVKKMKGPKILLVKVYPKMQDGE 393 KEGQK++F AFD+V+K+M GPKI+LVKVYPK+Q+ Sbjct: 404 KEGQKIDFPAFDVVIKRMSGPKIVLVKVYPKLQEAR 439 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 102 bits (253), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 87/381 (22%), Positives = 167/381 (43%), Gaps = 12/381 (3%) Query: 4 RILATVHNQLDAYLSACQLGITLASLGLGWIGEPAFASLLEPLFALLGVSSLELIHGVSF 63 R+L ++ Q + + Q+G+ ++ G +GEPA + + + + + SF Sbjct: 48 RVL-SLQAQPGNFFTVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPW--LENASF 104 Query: 64 VFAFVIISFLHIVVGELAPKSMAIRNPERIGLWCAMPLYGFYWMMYPAIWLLNLSANWVL 123 +F++++ I+ +L PK +A+ PER + P+ + P +W N + W+ Sbjct: 105 AMSFLVVTAFFILFADLMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIF 164 Query: 124 RVTGLGGVHGHDSHYSSDELKLILRSGGKTGKFTPQEWSVLAQSLDFGDLKVMDLMRPAN 183 + GL V + +E+ ++ +G + G QE ++ + V M P + Sbjct: 165 TLFGLPQV--RQEVITPNEIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRD 222 Query: 184 EIVALSAANTHDENMQIIYRNRFSRYPYFDAGQQEVLGLLHLKDVFIAQQDGK--AIVDL 241 IV ++ Q I + SR+ D VLG + KDV G+ ++ D Sbjct: 223 SIVYFLLTDSEATIRQKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD- 281 Query: 242 RHYLRPVQYISPSLSAQELLRRFRTGSPHFAIIGRKGQAPLGFVTLDNLLSVLVGEIRDE 301 + + P I SL+ E+L R + AI+ + +G ++ ++ VL+G + Sbjct: 282 KGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLL-A 340 Query: 302 FRHTSNEWTRQDDGTLLGKGSLPIVTLEHMLGIDFEYDETV--DSVGGLVMEKLGEVPKE 359 + R+D+ + L G P+ + L ID EY V +++ G +M L ++PK+ Sbjct: 341 ITEEEQQIVRRDENSWLMDGLTPLNDVMSELDID-EYPNPVGYETLSGFIMYMLRKIPKK 399 Query: 360 GQKVEFHAFDIVVKKMKGPKI 380 V F F V ++G +I Sbjct: 400 TDFVLFAEFKFEVVDIEGNRI 420 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 86/364 (23%), Positives = 162/364 (44%), Gaps = 14/364 (3%) Query: 29 LGLGWIGEPAFASLLEPLFALLGVSSLELIHGVSFVFAFVIISFLHIVVGELAPKSMAIR 88 L L I A +L L L +++ H A ++ L I+ E+ PK + Sbjct: 63 LSLVLIANTAINALATALVTALAIAAFG-NHQRVITAATACVAALLIIFAEIVPKVIGAT 121 Query: 89 NPERIGLWCAMPLYGFYWMMYPAIWLLNLSANWVLRVTGL-GGVHGHDSHYSSDELKLIL 147 PERI L+ L + P IW +NL + +LR+ + G + S +EL+ I+ Sbjct: 122 YPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIV 181 Query: 148 RSGGKTGKFTPQEW-SVLAQSLDFGDLKVMDLMRPANEIVALSAANTHDENMQIIYRNRF 206 G G F PQ+ S+L D + V D+M P +++ AL+ A D+ + + Sbjct: 182 LEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYH 238 Query: 207 SRYPYFDAGQQEVLGLLHLKDV--FIAQQDGKAIVDLRHYLRPVQYISPSLSAQELLRRF 264 ++ P F+ ++G+LH++ +++ + D+R L Y+ L+ F Sbjct: 239 NKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYF 298 Query: 265 RTGSPHFAIIGRKGQAPLGFVTLDNLLSVLVGEIRDEFRHTSNE---WTRQDDGTLLGKG 321 + ++ + G VTL++++ ++GE +S E W + DG L +G Sbjct: 299 QENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDK--DGACLLEG 356 Query: 322 SLPIVTLEHMLGIDFEYDETVDSVGGLVMEKLGEVPKEGQKVEFHAFDIVVKKMKGPKIL 381 S + + LG+ D ++ GL++E L ++P+ V+ I + +++ I Sbjct: 357 STTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIR 415 Query: 382 LVKV 385 +VK+ Sbjct: 416 MVKL 419 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 64/261 (24%), Positives = 115/261 (44%), Gaps = 25/261 (9%) Query: 142 ELKLILRSGGKTGKFTPQEWSVLAQSLDFGDLKVMDLMRPANEIVALSAANTHDENMQII 201 EL +L + S++ DL V D+M P +++ + + + M + Sbjct: 35 ELLEVLHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEV 94 Query: 202 YRNRFSRYPYFDAGQQEVLGLLHLKDV--FIAQQDGKAIVDLRHYLRPVQYISPSLSAQE 259 SR+P D + +V+G+L KD+ + A++ D+R LRP +I S Sbjct: 95 LATSHSRFPAVDGERDKVVGILLAKDLLRYYAEES----FDVRDMLRPAIFIPESKRLNV 150 Query: 260 LLRRFRTGSPHFAIIGRKGQAPLGFVTLDNLLSVLVGEIRDEFRHTSNEWTRQDDGTLLG 319 LLR FR H A++ + G +T++++L +VG+I DE+ ++ +D L Sbjct: 151 LLRDFRANRNHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEY-----DFDEAEDNVLAL 205 Query: 320 KGS-----------LPIVTLEHMLGIDFEYDETVDSVGGLVMEKLGEVPKEGQKVEFHA- 367 K I +G F D+ D++GGLV +G VP++G + + Sbjct: 206 KDGPHGARWRVKALTEIEQFNEEVGSRF-SDDDADTIGGLVANHVGRVPRKGDEFDIDGL 264 Query: 368 -FDIVVKKMKGPKILLVKVYP 387 F+++ + +LLV P Sbjct: 265 HFEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,321,123 Number of extensions: 57129 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 4 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)