BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0106.0001.p
         (159 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase...   278   1e-76

>gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 531

 Score =  278 bits (711), Expect = 1e-76,   Method: Compositional matrix adjust.
 Identities = 127/157 (80%), Positives = 144/157 (91%)

Query: 1   LSDDTLYLPYATSLRMSDLGYQSNAQANITPHYNNLHSYIRSLSQAVSQPYPAYEEIGTK 60
           LS+DTLYLPY TSLRMSDLGYQ++AQANITPHYNNL +YIRSLS AVS+PYP YE IGTK
Sbjct: 225 LSEDTLYLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLSHAVSEPYPPYEAIGTK 284

Query: 61  RNGEWIQINTNILQIENEYYSAIRPKRVIYSGERPVQALAARGVQYVEVRCMDIDPFEPL 120
           R+G+W+QINTNILQIENE+YS+IRPKRVI SGERPVQAL+ARGVQYVEVRCMDIDPF PL
Sbjct: 285 RDGQWVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPL 344

Query: 121 GVSLKASRFLDVFLHFIAFEESCMTSDASGAESRCNF 157
           G+ L+A+RFLDVFLHF+A E SC+TSD  GAE+R NF
Sbjct: 345 GIGLEAARFLDVFLHFLALESSCLTSDEEGAENRANF 381


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 434,296
Number of extensions: 15091
Number of successful extensions: 28
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 1
Length of query: 159
Length of database: 1,619,870
Length adjustment: 78
Effective length of query: 81
Effective length of database: 1,250,540
Effective search space: 101293740
Effective search space used: 101293740
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 127 (53.5 bits)