BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0105.0004.p (357 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 624 e-180 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 92 7e-20 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 75 8e-15 gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herb... 63 4e-11 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 624 bits (1609), Expect = e-180, Method: Compositional matrix adjust. Identities = 292/357 (81%), Positives = 323/357 (90%), Gaps = 1/357 (0%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKETFQYKQGQHLTLRSEINGEDLRRSYSIC 60 MSKFYPLT+ +VK ETRDTIVVSF VPAEL++TF Y+QGQHLTLRSEINGEDLRRSYSIC Sbjct: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAGNKKHYLAFAA 120 SAVQ+ QLRVAIKR GG+FS WAN++ PGQ + VMPPMGHFN+PL+AGN+KHYLAFAA Sbjct: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 Query: 121 GSGITPMLSIVKTTLLTEPDSRFTLVYGNRASSSVIFKEELTDLKDTYLERLNVVYVMSR 180 GSGITPM+SI+KTTLL+EP S FTLVY NRASSSVIFKEELTDLKD YLER NVVYVMSR Sbjct: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDAYLERFNVVYVMSR 180 Query: 181 EQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEDMLNAVSESLQANGLDKSAIK 240 EQQDVELFNGRI R KCD FF SWIDL D+DAAF+CGPE+M+ AV+ SLQA+GL K+ IK Sbjct: 181 EQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIK 240 Query: 241 VELFAASIPKHKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKHGIDMRY 300 ELFAAS P +A AAR VVGKQECEVTVIVDGYH VFTM+KEKES+L+AGLKHGID+RY Sbjct: 241 TELFAASTPP-RAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRY 299 Query: 301 SCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQDN 357 SCKGGVCATCRCK+VEGKVDMDANY LEDYEIARGFVLSCQSFPV+DKLL+DFDQDN Sbjct: 300 SCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 92.0 bits (227), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 67/237 (28%), Positives = 108/237 (45%), Gaps = 19/237 (8%) Query: 21 VVSFD-VPAELKETFQYKQGQHLTLRSEINGEDLRRSYSICS----AVQDDQLRVAIKRT 75 + SF VP + E + GQ++ LR ++GE+ RR YS+ + A Q Q R+++KR Sbjct: 170 ITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRISVKRE 229 Query: 76 QGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAGNKKHYLAFAAGSGITPMLSIVKTTL 135 GG S +D + G +++ PP G F L + K + G GITPM+S++ L Sbjct: 230 PGGKVSRQLHDQIHVGSVVELFPPAGEFTL---MASDKPLALISGGVGITPMMSMLAAAL 286 Query: 136 LTEPDSR-FTLVYGNRASSSVIFKEELTDLKDTYLE-RLNVVYVMSREQQDVELFNGRIT 193 P R ++ R F+ +L +L + + +L Y R+ G I Sbjct: 287 ---PSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGLIE 343 Query: 194 REKCDQFFKSWIDLDDIDAAFICGPEDMLNAVSESLQANGLDKSAIKVELF--AASI 248 R Q+ DLD A+ GP+ + A+ L G+ + + E F AAS+ Sbjct: 344 RSLLQQWLPGNPDLD----AYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPAASL 396 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 75.1 bits (183), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 72/312 (23%), Positives = 129/312 (41%), Gaps = 52/312 (16%) Query: 53 LRRSYSICSAVQDD-QLRVAIKR---TQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLD 108 L R YS+C+A + + ++ + R ++GG S ++ LK G L + P F L Sbjct: 48 LVRQYSLCNAPHEQHRYQIGVLRDVNSRGG--SVAVHEALKLGDILTISAPRNQFALV-- 103 Query: 109 AGNKKHYLAFAAGSGITPMLSIVKTTLLTEPDSRFTLVYGNRASSSVIFKEELTDLKDTY 168 +H L A G G+TP+L + + + F + Y R+ + F+E + Sbjct: 104 --PAQHSLLLAGGIGVTPILCMAEALAAAG--ASFEMHYCARSPARQAFRERIA------ 153 Query: 169 LERLNVVYVMSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAA-------FICGPEDM 221 F R+ D +DLD + A ++CGP Sbjct: 154 ----------------ASGFAARVVHHYDDGEAAQKLDLDALLAQADPATHLYVCGPAGF 197 Query: 222 LNAVSESLQANGLDKSAIKVELFAASIPKHKAVAARPVVGKQECEVTVIVDGYHTVFTME 281 + V ++ +A G ++ + E F A+ K G EV + G V+T+ Sbjct: 198 IAHVVDTARARGWPEAQVHFEYFGAAPVKAD--------GNGAFEVKLASSG--QVYTIP 247 Query: 282 KEKESLLDAGLKHGIDMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQ 341 E+ +++ +HG+D+ SC+ G+C TC +++EG+ D Y ++ A C Sbjct: 248 PER-TVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCC 306 Query: 342 SFPVTDKLLIDF 353 S + L++D Sbjct: 307 SRARSALLVLDL 318 >gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 52/187 (27%), Positives = 90/187 (48%), Gaps = 10/187 (5%) Query: 6 PLTVGNVKNETRDTIVVSFDVPAELKETFQYKQGQHLTLRSEINGEDLRRSYSICSAV-Q 64 P+ V + D IV+S +PA + QYK GQ++ + + RRSYS+ +A + Sbjct: 104 PVRVAKLDKVADDVIVLSLQLPA--NDRLQYKAGQYVEF---LLRDGKRRSYSMATAPHK 158 Query: 65 DDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAGNKKHYLAFAAGSGI 124 D+ + + I+ GG+F+ TLK L++ P+G F L D + K + A+G+G Sbjct: 159 DEHMSLHIRHLPGGLFTDQVFTTLKERDILRIEGPLGTFFLRED--SDKPIILLASGTGF 216 Query: 125 TPMLSIVKTTLLTEPDSRFTLVYGNRASSSVIFKEELTDLKDTYLERLNVVYVMSREQQD 184 P+ +IV+ TL +G R ++ + L + L V V+S Q + Sbjct: 217 APIKAIVEQLEHAGSKRPVTLYWGGRRPQD-LYMDALCQQWEQTLPNFKYVPVVSNAQAE 275 Query: 185 VELFNGR 191 + ++GR Sbjct: 276 -DNWSGR 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,037,393 Number of extensions: 40008 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 97 Number of HSP's successfully gapped: 4 Length of query: 357 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 271 Effective length of database: 1,212,660 Effective search space: 328630860 Effective search space used: 328630860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)