BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0103.0018.p
         (276 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309620|ref|YP_003773712.1| major facilitator superfamily p...   219   2e-58
gi|300309773|ref|YP_003773865.1| major facilitator superfamily p...   177   6e-46

>gi|300309620|ref|YP_003773712.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 405

 Score =  219 bits (559), Expect = 2e-58,   Method: Compositional matrix adjust.
 Identities = 130/265 (49%), Positives = 178/265 (67%), Gaps = 4/265 (1%)

Query: 2   VLFIGRLLMGPAESLFLTGTMTWGIGRVGMQRTGKVMAWQGIAMFAALGLGAPIGIAIQQ 61
           VL +GR+++   ESL +TG M+WGIG VG Q  GKVMAW GIA++AA G GAP+G+AI Q
Sbjct: 123 VLLLGRVVLALGESLMVTGAMSWGIGLVGPQHGGKVMAWLGIALYAAFGAGAPLGVAINQ 182

Query: 62  RFGFAGVALTTMVLPLVGLAIAATLRAL-PALGRKGGGVAMSEVLRLIWRFGLALALSAM 120
            +GFAG++L ++ L L  L + A LRA+ P+  R+       +VL  +WR GL LA S++
Sbjct: 183 IYGFAGISLASVGLSLAALLLIAPLRAIAPSAHRR---TPFYQVLGAVWRPGLGLAFSSV 239

Query: 121 PFAIITSFLVLLYATNNWSGAGIAILGFSAGYVGVRLFFAHLPDRIGGAKVGAVSVAIEL 180
            F +IT+F+ LL+A   W  A +A   F A ++  RL FAHLPD++GGA+V  V V IE 
Sbjct: 240 GFGVITAFIALLFAARAWGNAALAFSAFGAAFILARLLFAHLPDKLGGARVALVCVLIEA 299

Query: 181 AGQLLLWQSHDPVMAGLGTLLTGIGFSLVFPSMGVQAMARVPAHSRGLAVATFMAFIDLA 240
           AGQLL+WQ+     A LG  LTG G+SL FP  GV+AM R P  +RGLA+  ++AF+DL+
Sbjct: 300 AGQLLIWQADAAHWAYLGAALTGFGYSLAFPGFGVEAMRRAPPQTRGLAMGAYVAFLDLS 359

Query: 241 AGLTGPVVGVLIGYFGYGAAFLAGA 265
            G+T P+ GVL  + G  + +LAGA
Sbjct: 360 LGITSPLAGVLASHAGVQSIYLAGA 384


>gi|300309773|ref|YP_003773865.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  177 bits (450), Expect = 6e-46,   Method: Compositional matrix adjust.
 Identities = 112/262 (42%), Positives = 161/262 (61%), Gaps = 2/262 (0%)

Query: 1   VVLFIGRLLMGPAESLFLTGTMTWGIGRVGMQRTGKVMAWQGIAMFAALGLGAPIGIAIQ 60
           V+LF  R L+G  ESL  TG + WG+GRVG + T K+++W GIA +AAL +GAP+G+ + 
Sbjct: 128 VLLFASRALLGLGESLVGTGAIMWGVGRVGSENTAKMISWNGIATYAALAIGAPLGVVLD 187

Query: 61  QRFGFAGVALTTMVLPLVGLAIAATLRALPALGRKGGGVAMSEVLRLIWRFGLALALSAM 120
           +  GFA + L+ M+L  +G  +A      P +  +G  ++   VLR ++ +G+ LA   +
Sbjct: 188 RSLGFAMIGLSGMLLTALGYVLARGGPVTPVV--QGEQLSFKLVLRKVFAYGMGLAFGTI 245

Query: 121 PFAIITSFLVLLYATNNWSGAGIAILGFSAGYVGVRLFFAHLPDRIGGAKVGAVSVAIEL 180
            F  I +F+ L YA  +WSGA  A+  FS+ +VG RL FA+   R GG +V  VS   E 
Sbjct: 246 GFGSIATFITLYYADLHWSGAAFALTAFSSAFVGARLLFANAITRFGGYRVAVVSFLTEA 305

Query: 181 AGQLLLWQSHDPVMAGLGTLLTGIGFSLVFPSMGVQAMARVPAHSRGLAVATFMAFIDLA 240
            G LLLW +  P +A LG  L G GF+LVFPS+GV+A+  V   +RG A+A +  F+DLA
Sbjct: 306 VGLLLLWSTTTPELALLGAGLAGFGFALVFPSLGVEAVNLVSPANRGAALAAYSVFLDLA 365

Query: 241 AGLTGPVVGVLIGYFGYGAAFL 262
            G+TGP+ G + G  GY   FL
Sbjct: 366 LGITGPLAGAIAGQLGYPQVFL 387


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.329    0.143    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 846,668
Number of extensions: 36400
Number of successful extensions: 282
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 280
Number of HSP's successfully gapped: 2
Length of query: 276
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 192
Effective length of database: 1,222,130
Effective search space: 234648960
Effective search space used: 234648960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 130 (54.7 bits)