BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0103.0006.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 208 9e-55 gi|300311136|ref|YP_003775228.1| threonine efflux protein [Herba... 145 1e-35 gi|300311137|ref|YP_003775229.1| drug/metabolite transporter (DM... 107 3e-24 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 82 9e-17 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 77 4e-15 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 208 bits (529), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 109/149 (73%), Positives = 121/149 (81%), Gaps = 2/149 (1%) Query: 218 AEAWVKHENHTPTGAFKIRGGLVYLQNLAQRPRQQQVRGVISATRGNHGQSVGFAARRYG 277 AE WVKHENH PTGAFK+RGG+VYL +LA++ Q Q+ GV+SATRGNHGQSVG AARR G Sbjct: 43 AEVWVKHENHAPTGAFKVRGGMVYLHHLARQ--QPQLSGVVSATRGNHGQSVGLAARRLG 100 Query: 278 IPATIVVPQGNSREKNAAMRALGVELVEFGQEFQESREHAALLAQQQQLHMIPSFHRDLV 337 I ATIVVPQGNSREKNAAMRALGVEL+E G EFQESRE+A LA QQQLHMIPS HRDLV Sbjct: 101 ISATIVVPQGNSREKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMIPSLHRDLV 160 Query: 338 SGVATYWMELLRAQPDXAGAAFAVGHPSG 366 +GVA+YWMEL AQP +G SG Sbjct: 161 AGVASYWMELFAAQPGLDVVLVPIGQGSG 189 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 71/113 (62%), Positives = 84/113 (74%), Gaps = 1/113 (0%) Query: 18 VYAAMPATPQYAWPLLSEALGAEAWXAARRALNVRTRIIGVVSAHALAYKLSFDGRSKIE 77 ++AA P P+ + G AAR AL + TRIIGVVSAHALAYKLSF+ KIE Sbjct: 170 LFAAQPGLDVVLVPI-GQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIE 228 Query: 78 SPVTTVIADGMACRVPDEASLEVILSEVDEVIAVSDDEVAAAMKLYFTATHNV 130 SPV+T IADG+ACRVPD+ASLEV+ SEVDEV+AV+DDEV AMKL + ATHNV Sbjct: 229 SPVSTCIADGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNV 281 >gi|300311136|ref|YP_003775228.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 145 bits (365), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 70/92 (76%), Positives = 79/92 (85%) Query: 369 LMEALLPLASFAFVTSITPGPNNIMLTSSGVWFGFLRSIPHMLGITFGFVVLLAICAAGI 428 ++E LLPL SFAFV+SITPGPNNIMLTSSG+WFGF RS+PHMLGITFGF VLLA+CA GI Sbjct: 1 MIETLLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGI 60 Query: 429 GSLVIAVPAIHVLLKIAGSGYLLYLAWKLRSM 460 G LVIAVP + +LLK G YLLYLAW+LR M Sbjct: 61 GELVIAVPQLVLLLKAMGCAYLLYLAWQLRRM 92 >gi|300311137|ref|YP_003775229.1| drug/metabolite transporter (DMT) superfamily permease [Herbaspirillum seropedicae SmR1] Length = 306 Score = 107 bits (266), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 50/78 (64%), Positives = 62/78 (79%) Query: 469 HVRXFALFAAVVAAAMGYAEGGRLSQSMGGQQVICWALVLSMPVVLPVTIWLGMHYGVQA 528 H FALFAAV+ AAMGYAEGGRL+QSMGGQ VI WALV+++PV+LPV++WLG G+ A Sbjct: 164 HAADFALFAAVITAAMGYAEGGRLAQSMGGQNVIAWALVVALPVMLPVSLWLGWQDGLSA 223 Query: 529 SPQAWMSFAYVSFFSTLL 546 S AW++F YVS FS + Sbjct: 224 SAPAWLAFGYVSLFSMFI 241 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 82.4 bits (202), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 52/140 (37%), Positives = 76/140 (54%), Gaps = 4/140 (2%) Query: 221 WVKHENHTPTGAFKIRGGLVYLQNLAQRPRQQQVRGVISATRGNHGQSVGFAARRYGIPA 280 + K E+ +FK+RG + +L P Q + RGVI A+ GNH Q V +A R G A Sbjct: 38 YFKREDMQSVFSFKLRGAYNKMAHLT--PAQLK-RGVICASAGNHAQGVALSAARLGCRA 94 Query: 281 TIVVPQGNSREKNAAMRALGVELVEFGQEFQESREHAALLAQQQQLHMIPSFHRD-LVSG 339 IV+P + K A++A G E+V FG F ++ EHA L ++Q+L + F +++G Sbjct: 95 VIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAG 154 Query: 340 VATYWMELLRAQPDXAGAAF 359 T ME+LR PD A F Sbjct: 155 QGTVGMEILRQHPDPIHAIF 174 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 76.6 bits (187), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 48/133 (36%), Positives = 72/133 (54%), Gaps = 4/133 (3%) Query: 218 AEAWVKHENHTPTGAFKIRGGLVYLQNLAQRPRQQQVRGVISATRGNHGQSVGFAARRYG 277 A+ + K EN GAFK RG + L Q Q+ GVI+ + GNH Q AA+ G Sbjct: 44 AQLFFKCENFQRMGAFKFRGAM---NALTQFDATQRKHGVITFSSGNHAQGTALAAQLLG 100 Query: 278 IPATIVVPQGNSREKNAAMRALGVELVEFGQEFQESREHAALLAQQQQLHMIPSF-HRDL 336 IPA IV+PQ K AA R G E+V + + ++ A LA ++++ +IP + H + Sbjct: 101 IPAVIVMPQDAPAAKVAATRGYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHV 160 Query: 337 VSGVATYWMELLR 349 ++G T +ELL+ Sbjct: 161 MAGQGTATLELLQ 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,736,543 Number of extensions: 72041 Number of successful extensions: 295 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 291 Number of HSP's successfully gapped: 8 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)