BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0103.0002.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [He... 259 2e-70 >gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [Herbaspirillum seropedicae SmR1] Length = 2656 Score = 259 bits (661), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 162/249 (65%), Positives = 199/249 (79%), Gaps = 7/249 (2%) Query: 1 VVVDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGT 60 VVVDLFH TGSA +G S++ GDTYS+IQNVVGS+ NDTFY AN F G GG Sbjct: 2361 VVVDLFHGTGSA----PSGVANSYAAGDTYSHIQNVVGSAGNDTFYASGDANAFTGGGG- 2415 Query: 61 SDTVSYEYSNANGAIIANLGT-HAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANIL 119 SDTVSY YS + G I +L T G+GGDA+GDTY+ I NL GSA ++S+LTG+A+AN L Sbjct: 2416 SDTVSYYYSTS-GDITVSLATGSVGSGGDASGDTYSGIANLEGSANVNSSLTGNASANTL 2474 Query: 120 TAKGTATTNAFSGGGAASGTDVFNVVDGGTNSVAVGTGLNTINVSAGSHSSLGAQSDMVN 179 TA+G +TTN+ SGGGA+SGTD++NVV+GG N+V VG+G N INVSAGSHSSLGAQSDMVN Sbjct: 2475 TARGDSTTNSLSGGGASSGTDIYNVVEGGHNTVTVGSGNNLINVSAGSHSSLGAQSDMVN 2534 Query: 180 QTTGLSNISTINGAAGTTTLHFADLGSSITLANFANKVTGITTLDMTSGVGTNIVITAAD 239 Q TG SNI++I+G AG TTLHFADLG+S+ L+NF++KV+GITTLD+++G TNI+ITA D Sbjct: 2535 QNTGQSNINSISGGAGVTTLHFADLGNSLNLSNFSSKVSGITTLDVSAGGSTNILITAQD 2594 Query: 240 VISMGMPXG 248 VI MGMP G Sbjct: 2595 VIRMGMPSG 2603 Score = 84.7 bits (208), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 58/119 (48%), Positives = 77/119 (64%), Gaps = 14/119 (11%) Query: 1 VVVDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGT 60 V VDLF+ GS G F+ GDTY+NIQNV+GS+Y+DTF D+ N FDG G Sbjct: 1045 VTVDLFNGRGSNG----------FAAGDTYANIQNVIGSAYDDTFVADNNHNVFDGRQG- 1093 Query: 61 SDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANIL 119 S+TVS Y+N+ A+ NL T GTGG A+GDT+TNI+N+ GSA D + ++AN + Sbjct: 1094 SNTVS--YANSTAAVTVNLVTGTGTGGFADGDTFTNIQNVVGSA-QDDLIIASSDANTI 1149 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 51/90 (56%), Positives = 64/90 (71%), Gaps = 3/90 (3%) Query: 27 GDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAIIANLGTHAGTG 86 GD SNIQN+ GS+Y+DTF ++ N+ DG G SDTVSYE SNA A+ NL T G+G Sbjct: 498 GDQLSNIQNLKGSAYSDTFVANASVNRLDGGSGGSDTVSYESSNA--AVTVNLVTGTGSG 555 Query: 87 GDANGDTYTNIENLTGSAFLDSTL-TGDAN 115 G A GDTYT+I+N+ GSAF D + +GDAN Sbjct: 556 GHAQGDTYTHIQNVVGSAFNDIFVASGDAN 585 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 85/248 (34%), Positives = 126/248 (50%), Gaps = 46/248 (18%) Query: 3 VDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSD 62 VDLF GS GG S + GDTY+NIQ+ G S NDTF + AN FDG GG+ + Sbjct: 1886 VDLFLGQGS-------GGAGSLANGDTYTNIQDATGGSANDTFVASAAANAFDGGGGSHN 1938 Query: 63 TVSYEYSNANGAIIANLGTHAGTGGD---ANGDTYTNIENLTGSAFLDSTLTGDANANIL 119 VSY S + I NL ++ GTGG ANGDTY NI+++TG + D T+ A AN L Sbjct: 1939 RVSYANSTVDETI--NLVSNTGTGGAGSLANGDTYANIQDVTGGSGND-TIVASAAANFL 1995 Query: 120 TAKGTATTNAFSGGGAASGTDVFNVVDGGTNSVAVGTGLNTINVSAGSHSSLGAQ----- 174 + T +++G +++G+ TG+ T+++SAG+ S AQ Sbjct: 1996 DGGTGSNTVSYAGSTSSNGS----------------TGV-TVDLSAGTGSGNYAQGDTYA 2038 Query: 175 -------SDMVNQTTGLSNI---STINGAAGTTTLHFADLGSSITLANFANKVTGITTLD 224 SD + TG + + S + G G L D + T A++A G+ +++ Sbjct: 2039 NIQNVIGSDFADTLTGFATVGVTSVLTGGKGADKLTAIDANRAYTYASYAGSANGV-SIN 2097 Query: 225 MTSGVGTN 232 + +GVG+N Sbjct: 2098 LQTGVGSN 2105 Score = 72.4 bits (176), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 51/119 (42%), Positives = 68/119 (57%), Gaps = 15/119 (12%) Query: 27 GDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNAN---GAIIANLGTHA 83 GDT NI N++GSS NDTFY + ANK DG G SDTV+Y S ++ + +L + Sbjct: 2110 GDTLFNIDNLIGSSQNDTFYASANANKLDGGAG-SDTVNYSLSTSSDGVNGVTVDLSAGS 2168 Query: 84 GTGGDANGDTYTNIENLTGSAFLD-----------STLTGDANANILTAKGTATTNAFS 131 G+G A+GDTYTNIEN GSA+ D S L G A A+++TA N ++ Sbjct: 2169 GSGNYADGDTYTNIENAVGSAYQDRLTGFATVGVRSVLEGGAGADVITAVAANRANTYA 2227 Score = 70.5 bits (171), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 50/130 (38%), Positives = 74/130 (56%), Gaps = 13/130 (10%) Query: 1 VVVDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGT 60 V VD+ + GS G ++ ++Y++IQN+VGSSY+DTF AN F+G G Sbjct: 729 VTVDMINLVGSGG----------YAQNNSYTHIQNIVGSSYSDTFIASLAANNFNGGLGG 778 Query: 61 SDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILT 120 SDTVSY +SNA A+ +L G+GG A GD +I+N+ GS + D T A+ N Sbjct: 779 SDTVSYAFSNA--AVTVDLFHGTGSGGYAQGDVLAHIQNVIGSNY-DDTFIASADVNNFD 835 Query: 121 AKGTATTNAF 130 +G + T ++ Sbjct: 836 GRGGSNTVSY 845 Score = 70.5 bits (171), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 52/113 (46%), Positives = 64/113 (56%), Gaps = 13/113 (11%) Query: 23 SFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAIIANLGTH 82 + + GDTY NIQ+V G NDTFYG++ AN FDG GGT + VSY S + LG Sbjct: 1834 TLAAGDTYLNIQDVTGGGGNDTFYGNTAANTFDGGGGTHNRVSYATSTVAETVDLFLGQG 1893 Query: 83 AGTGGD-ANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGTATTNAFSGGG 134 +G G ANGDTYTNI++ TG G AN + +A NAF GGG Sbjct: 1894 SGGAGSLANGDTYTNIQDATG---------GSANDTFVA---SAAANAFDGGG 1934 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 79/233 (33%), Positives = 110/233 (47%), Gaps = 33/233 (14%) Query: 1 VVVDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGT 60 V VDL G+ G ++ DTY+NIQNV GS ++DTF AN FDG G Sbjct: 982 VTVDLVLGVGTGG----------YANNDTYANIQNVTGSGFDDTFIASLAANAFDGGAG- 1030 Query: 61 SDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILT 120 ++TVSY + + +L G+ G A GDTY NI+N+ GSA+ D T D N N+ Sbjct: 1031 NNTVSYAKAGDGNGVTVDLFNGRGSNGFAAGDTYANIQNVIGSAY-DDTFVADNNHNVFD 1089 Query: 121 AKGTATTNAFSGGGAASGTDVFNVVDG-GTNSVAVGTGLNTINVSAGSHSSLGAQSDMVN 179 + + T +++ AA N+V G GT A G I GS AQ D++ Sbjct: 1090 GRQGSNTVSYANSTAAV---TVNLVTGTGTGGFADGDTFTNIQNVVGS-----AQDDLII 1141 Query: 180 QTTGLSNISTINGAAGTTTLHFADLGSSITLANFANKVTGITT-LDMTSGVGT 231 + S+ +TI+G T H ++AN G+T L T G GT Sbjct: 1142 AS---SDANTIDGGLSATGAH--------NRVSYANSGAGVTVDLTYTDGTGT 1183 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 48/114 (42%), Positives = 65/114 (57%), Gaps = 17/114 (14%) Query: 24 FSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAIIANLGTHA 83 + GDTY+NIQ+ G S NDTFYG++ AN+FDG GG+ + V+Y S A +L + Sbjct: 1381 LANGDTYANIQDATGGSGNDTFYGNTAANRFDGGGGSHNRVNYSGSTV--AETVDLFNNV 1438 Query: 84 GTGGD---ANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGTATTNAFSGGG 134 G+GG ANGDTY NI+++TG D + +A NAF GGG Sbjct: 1439 GSGGAGSLANGDTYANIQDVTGGTADDVFIA------------SAAANAFDGGG 1480 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 47/112 (41%), Positives = 62/112 (55%), Gaps = 13/112 (11%) Query: 24 FSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAI-IANLGTH 82 F+ GD Y+NIQ+V G S NDTF + AN+FDG GG+ + VSY S + + + N Sbjct: 1708 FAAGDVYTNIQDVTGGSANDTFIASAAANRFDGGGGSHNRVSYASSTVSETVDLFNGNGT 1767 Query: 83 AGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGTATTNAFSGGG 134 G G ANGDTYTNI+++TG D + +A NAF GGG Sbjct: 1768 GGAGSLANGDTYTNIQDVTGGTADDVFIA------------SAAANAFDGGG 1807 Score = 65.5 bits (158), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 41/80 (51%), Positives = 50/80 (62%), Gaps = 1/80 (1%) Query: 24 FSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAI-IANLGTH 82 + GDTY NIQ+V G S NDTFYG++ AN FDG GGT + V+Y S I + N Sbjct: 1512 LANGDTYINIQDVTGGSGNDTFYGNTAANNFDGGGGTHNRVNYSSSTVAETIDLLNGQGS 1571 Query: 83 AGTGGDANGDTYTNIENLTG 102 G G ANGD YTNI++ TG Sbjct: 1572 GGAGSLANGDRYTNIQDATG 1591 Score = 65.5 bits (158), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 62/156 (39%), Positives = 83/156 (53%), Gaps = 11/156 (7%) Query: 3 VDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSD 62 VDLF++ GS GV S + GDTY+NIQ+V G S +D F + AN FDG GG+ + Sbjct: 1302 VDLFNNVGSGGVG-------SLANGDTYANIQDVTGGSADDLFVASAAANAFDGGGGSHN 1354 Query: 63 TVSYEYSNANGAI-IANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTA 121 VSY S + + + + G G ANGDTY NI++ TG + D T G+ AN Sbjct: 1355 RVSYANSTVDETVNLVSGQGSGGIGSLANGDTYANIQDATGGSGND-TFYGNTAANRFDG 1413 Query: 122 KGTATTNA-FSGGGAASGTDVFNVV-DGGTNSVAVG 155 G + +SG A D+FN V GG S+A G Sbjct: 1414 GGGSHNRVNYSGSTVAETVDLFNNVGSGGAGSLANG 1449 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 50/103 (48%), Positives = 60/103 (58%), Gaps = 13/103 (12%) Query: 1 VVVDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGT 60 V VDL + TGS G DA GDT NI NV+GS+Y+DTF + AN FDG G Sbjct: 2236 VTVDLKNATGSGG-DA---------QGDTLVNIDNVIGSAYSDTFVASTQANNFDGGSGG 2285 Query: 61 SDTVSYEYSNANGAIIANL-GTHAGTGGDANGDTYTNIENLTG 102 SDTVS Y+N G + +L T A +G A GD +TNI NL G Sbjct: 2286 SDTVS--YANDTGGVTVDLTNTLAASGAYAVGDKFTNIANLIG 2326 Score = 61.2 bits (147), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 41/116 (35%), Positives = 68/116 (58%), Gaps = 2/116 (1%) Query: 27 GDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAIIANLGTHAGTG 86 GDTY++IQN +GS+++D F+ + FDG G SDTV Y + GA+ ++ G+G Sbjct: 683 GDTYNHIQNAIGSAFDDVFFAGVDSRHFDGGSGGSDTV--NYGGSTGAVTVDMINLVGSG 740 Query: 87 GDANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGTATTNAFSGGGAASGTDVF 142 G A ++YT+I+N+ GS++ D+ + A N G + T +++ AA D+F Sbjct: 741 GYAQNNSYTHIQNIVGSSYSDTFIASLAANNFNGGLGGSDTVSYAFSNAAVTVDLF 796 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 53/133 (39%), Positives = 69/133 (51%), Gaps = 20/133 (15%) Query: 3 VDLFHHTGSAGVDAAAGGGVSFSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSD 62 +DL++ TGS G++ S + GDTY+NIQ+V G + NDTF AN FDG GGT + Sbjct: 1629 IDLYNGTGSGGLN-------SLANGDTYANIQDVTGGAGNDTFVATVAANTFDGGGGTHN 1681 Query: 63 TVSYEYSN-ANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTA 121 VSY S A + G G A GD YTNI+++TG G AN + Sbjct: 1682 RVSYANSTVAETVDLIAGLGSGGAGSFAAGDVYTNIQDVTG---------GSANDTFIA- 1731 Query: 122 KGTATTNAFSGGG 134 +A N F GGG Sbjct: 1732 --SAAANRFDGGG 1742 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 39/87 (44%), Positives = 53/87 (60%), Gaps = 3/87 (3%) Query: 24 FSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAGGTSDTVSYEYSNANGAIIANLGTHA 83 ++ GD +NIQ++ G + NDTF N FDG G S+ VSY S + A+ +L Sbjct: 934 YAQGDKLANIQDLTGGAGNDTFVASLAENNFDGGAG-SNRVSYAASTS--AVTVDLVLGV 990 Query: 84 GTGGDANGDTYTNIENLTGSAFLDSTL 110 GTGG AN DTY NI+N+TGS F D+ + Sbjct: 991 GTGGYANNDTYANIQNVTGSGFDDTFI 1017 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 45/108 (41%), Positives = 59/108 (54%), Gaps = 6/108 (5%) Query: 3 VDLFHHTGSAGVDAAAGGGVS----FSTGDTYSNIQNVVGSSYNDTFYGDSIANKFDGAG 58 V F+ TG VD G ++ GD++SNIQN++GS Y+DTF + AN FDG Sbjct: 843 VSYFYSTGGVTVDLTNTIGTGTGGYYAAGDSFSNIQNLIGSVYDDTFVASADANSFDGGT 902 Query: 59 GT-SDTVSYEYSNANGAIIANLGTHAGT-GGDANGDTYTNIENLTGSA 104 G+ + VSY S A + N GT GG A GD NI++LTG A Sbjct: 903 GSLHNRVSYAASTAGVTVDLNYSNGTGTSGGYAQGDKLANIQDLTGGA 950 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.127 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 799,724 Number of extensions: 34989 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 20 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 130 (54.7 bits)