BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0103.0001.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [He... 202 3e-53 >gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [Herbaspirillum seropedicae SmR1] Length = 2656 Score = 202 bits (514), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 149/277 (53%), Positives = 186/277 (67%), Gaps = 38/277 (13%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAS- 59 VDL TN SG YA GD+F NI ++ G SGNDTF A+ AN F+GG G +TV+Y+ S Sbjct: 2300 VDL--TNTLAASGAYAVGDKFTNIANLIGGSGNDTFYASSAANAFEGGAGVNTVNYARSG 2357 Query: 60 TVSVTVDLSSG------------GAG---------VGEGGXGA------------GGTSD 86 + V VDL G AG VG G GG SD Sbjct: 2358 SAGVVVDLFHGTGSAPSGVANSYAAGDTYSHIQNVVGSAGNDTFYASGDANAFTGGGGSD 2417 Query: 87 TVSYEYSNANGAIIANLGT-HAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTA 145 TVSY YS + G I +L T G+GGDA+GDTY+ I NL GSA ++S+LTG+A+AN LTA Sbjct: 2418 TVSYYYSTS-GDITVSLATGSVGSGGDASGDTYSGIANLEGSANVNSSLTGNASANTLTA 2476 Query: 146 KGTATTNAFSGGGAASGTDVFNVVDGGTNSVAVGTGLNTINVSAGSHSSLGAQSDMVNQT 205 +G +TTN+ SGGGA+SGTD++NVV+GG N+V VG+G N INVSAGSHSSLGAQSDMVNQ Sbjct: 2477 RGDSTTNSLSGGGASSGTDIYNVVEGGHNTVTVGSGNNLINVSAGSHSSLGAQSDMVNQN 2536 Query: 206 TGLSNISTINGAAGTTTLHFADLGSSITLANFANKVT 242 TG SNI++I+G AG TTLHFADLG+S+ L+NF++KV+ Sbjct: 2537 TGQSNINSISGGAGVTTLHFADLGNSLNLSNFSSKVS 2573 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 100/269 (37%), Positives = 137/269 (50%), Gaps = 50/269 (18%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 VDLN +NG+GTSGGYA GD+ NIQD+TG +GNDTF+A+ NNFDGG GS+ VSY+AST Sbjct: 920 VDLNYSNGTGTSGGYAQGDKLANIQDLTGGAGNDTFVASLAENNFDGGAGSNRVSYAAST 979 Query: 61 VSVTVDLSSGGAGVGEGGXG------------------------------AGGTSDTVSY 90 +VTVDL GVG GG G ++TVSY Sbjct: 980 SAVTVDLV---LGVGTGGYANNDTYANIQNVTGSGFDDTFIASLAANAFDGGAGNNTVSY 1036 Query: 91 EYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGTAT 150 + + +L G+ G A GDTY NI+N+ GSA+ D T D N N+ + + Sbjct: 1037 AKAGDGNGVTVDLFNGRGSNGFAAGDTYANIQNVIGSAY-DDTFVADNNHNVFDGRQGSN 1095 Query: 151 TNAFSGGGAASGTDVFNVVDG-GTNSVAVGTGLNTINVSAGSHSSLGAQSDMVNQTTGLS 209 T +++ AA N+V G GT A G I GS AQ D++ + S Sbjct: 1096 TVSYANSTAAV---TVNLVTGTGTGGFADGDTFTNIQNVVGS-----AQDDLIIAS---S 1144 Query: 210 NISTINGAAGTTTLH----FADLGSSITL 234 + +TI+G T H +A+ G+ +T+ Sbjct: 1145 DANTIDGGLSATGAHNRVSYANSGAGVTV 1173 Score = 82.4 bits (202), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/192 (39%), Positives = 102/192 (53%), Gaps = 48/192 (25%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGS--DTVSYSA 58 VDL T+G+GTSGGYA GD+ NIQD+TGS +DTF+A+ ANNFDGG GS + VSY+ Sbjct: 1173 VDLTYTDGTGTSGGYATGDKLSNIQDLTGSQFDDTFVASLAANNFDGGTGSLHNRVSYAH 1232 Query: 59 STVSVTVDLSSGGAGVGEGGXG---------AGGTSD---------------------TV 88 S+ S T+DL + G+G G G GG++D V Sbjct: 1233 SSASETIDLFN-GSGTGTYAQGDRYTNIQDVTGGSADDLIIANAAANNIDGGGGSLHNRV 1291 Query: 89 SYEYSNANGAI--IANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTAK 146 SY+ S + + N+G+ G G ANGDTY NI+++TG + D + A Sbjct: 1292 SYQNSTVSETVDLFNNVGS-GGVGSLANGDTYANIQDVTGGSADD----------LFVA- 1339 Query: 147 GTATTNAFSGGG 158 +A NAF GGG Sbjct: 1340 -SAAANAFDGGG 1350 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 70/159 (44%), Positives = 89/159 (55%), Gaps = 36/159 (22%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGS-DTVSYSAS 59 VDL + GSG +G +AAGD + NIQD+TG S NDTFIA+ AN FDGG GS + VSY++S Sbjct: 1694 VDLIAGLGSGGAGSFAAGDVYTNIQDVTGGSANDTFIASAAANRFDGGGGSHNRVSYASS 1753 Query: 60 TVSVTVDLSSGGAGVGEGGXGA--------------GGTSDTV----------------- 88 TVS TVDL +G G GG G+ GGT+D V Sbjct: 1754 TVSETVDLFNGN---GTGGAGSLANGDTYTNIQDVTGGTADDVFIASAAANAFDGGGGTH 1810 Query: 89 -SYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTG 126 Y+N+N A NL + G+G A GDTY NI+++TG Sbjct: 1811 NRVSYANSNAAETINLVSGQGSGTLAAGDTYLNIQDVTG 1849 Score = 79.0 bits (193), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 69/167 (41%), Positives = 88/167 (52%), Gaps = 37/167 (22%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 V +N G+G SGG+A GD + +IQ++ GS+ ND F+A+G AN FDGG GS+TVSY S Sbjct: 544 VTVNLVTGTG-SGGHAQGDTYTHIQNVVGSAFNDIFVASGDANRFDGGAGSNTVSYVNSA 602 Query: 61 VSVTVDLSSGGAGVGEGGXGAGGT-------------------------------SDTVS 89 V V V+L + GVG GG G T +DTVS Sbjct: 603 VGVNVNLLT---GVGSGGAADGDTYVRIQNVIGTAGDDTFVGNADANSFDGGSGGNDTVS 659 Query: 90 YEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTG 136 Y S A G + N + GTGG+A GDTY +I+N GSAF D G Sbjct: 660 YAGS-AQG-VSVNFISGRGTGGNAEGDTYNHIQNAIGSAFDDVFFAG 704 Score = 77.0 bits (188), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 77/192 (40%), Positives = 95/192 (49%), Gaps = 54/192 (28%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 ++L S G+G +G A GD + NIQD+TG SGNDT +A+ AN DGG GS+TVSY+ ST Sbjct: 1951 INLVSNTGTGGAGSLANGDTYANIQDVTGGSGNDTIVASAAANFLDGGTGSNTVSYAGST 2010 Query: 61 VS-----VTVDLSSGGAGVGEGGXGAGGT------------SDTV--------------- 88 S VTVDLS AG G G G T +DT+ Sbjct: 2011 SSNGSTGVTVDLS---AGTGSGNYAQGDTYANIQNVIGSDFADTLTGFATVGVTSVLTGG 2067 Query: 89 -------------SYEYS----NANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLD 131 +Y Y+ +ANG I NL T G+ GDA GDT NI+NL GS+ D Sbjct: 2068 KGADKLTAIDANRAYTYASYAGSANGVSI-NLQTGVGSNGDAQGDTLFNIDNLIGSSQND 2126 Query: 132 STLTGDANANIL 143 T ANAN L Sbjct: 2127 -TFYASANANKL 2137 Score = 72.8 bits (177), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 61/140 (43%), Positives = 76/140 (54%), Gaps = 37/140 (26%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 V++N G GT GG A GD+ NIQ++ GS+ +DTF+AN N DGG Sbjct: 482 VNVNLVTGVGT-GGDAQGDQLSNIQNLKGSAYSDTFVANASVNRLDGG------------ 528 Query: 61 VSVTVDLSSGGAGVGEGGXGAGGTSDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTN 120 SGG SDTVSYE SNA A+ NL T G+GG A GDTYT+ Sbjct: 529 --------SGG-------------SDTVSYESSNA--AVTVNLVTGTGSGGHAQGDTYTH 565 Query: 121 IENLTGSAFLDSTL-TGDAN 139 I+N+ GSAF D + +GDAN Sbjct: 566 IQNVVGSAFNDIFVASGDAN 585 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 64/171 (37%), Positives = 81/171 (47%), Gaps = 53/171 (30%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 V +N G G++G A GD NI ++ GSS NDTF A+ AN DGG GSDTV+YS ST Sbjct: 2094 VSINLQTGVGSNGD-AQGDTLFNIDNLIGSSQNDTFYASANANKLDGGAGSDTVNYSLST 2152 Query: 61 VS-----VTVDLSSGGAGVGEGGXGAGGTSDTVSYEYSNANGAIIANLGTHAGTGGDANG 115 S VTVDLS+G +G+G A+G Sbjct: 2153 SSDGVNGVTVDLSAG------------------------------------SGSGNYADG 2176 Query: 116 DTYTNIENLTGSAFLD-----------STLTGDANANILTAKGTATTNAFS 155 DTYTNIEN GSA+ D S L G A A+++TA N ++ Sbjct: 2177 DTYTNIENAVGSAYQDRLTGFATVGVRSVLEGGAGADVITAVAANRANTYA 2227 Score = 67.8 bits (164), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 68/172 (39%), Positives = 87/172 (50%), Gaps = 36/172 (20%) Query: 16 AAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGS-DTVSYSASTVSVTVDLSSGGAGV 74 A GD ++NIQD+TG SGNDTF N ANNFDGG G+ + V+YS+STV+ T+DL + G Sbjct: 1513 ANGDTYINIQDVTGGSGNDTFYGNTAANNFDGGGGTHNRVNYSSSTVAETIDLLN---GQ 1569 Query: 75 GEGGXGA--------------GGTSDTVSYEYSNAN-----GAIIANLGTHAGTGGDANG 115 G GG G+ GG++D V + AN G + N ++A + D Sbjct: 1570 GSGGAGSLANGDRYTNIQDATGGSADDVFIASAAANRFDGGGGSLHNRVSYASSTADETI 1629 Query: 116 DTYTNIENLTGSAFLDSTLTGDANANILTAKG---------TATTNAFSGGG 158 D Y N TGS L+S GD ANI G T N F GGG Sbjct: 1630 DLY----NGTGSGGLNSLANGDTYANIQDVTGGAGNDTFVATVAANTFDGGG 1677 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 67/199 (33%), Positives = 101/199 (50%), Gaps = 38/199 (19%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLG-SDTVSYSAS 59 V++N G G SGG A GD +V IQ++ G++G+DTF+ N AN+FDGG G +DTVSY+ S Sbjct: 605 VNVNLLTGVG-SGGAADGDTYVRIQNVIGTAGDDTFVGNADANSFDGGSGGNDTVSYAGS 663 Query: 60 TVSVTVDLSSGGAGVGEGGXGAGGT-------------------------------SDTV 88 V+V+ SG G GG G T SDTV Sbjct: 664 AQGVSVNFISG---RGTGGNAEGDTYNHIQNAIGSAFDDVFFAGVDSRHFDGGSGGSDTV 720 Query: 89 SYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLDSTLTGDANANILTAKGT 148 Y + GA+ ++ G+GG A ++YT+I+N+ GS++ D+ + A N G Sbjct: 721 --NYGGSTGAVTVDMINLVGSGGYAQNNSYTHIQNIVGSSYSDTFIASLAANNFNGGLGG 778 Query: 149 ATTNAFSGGGAASGTDVFN 167 + T +++ AA D+F+ Sbjct: 779 SDTVSYAFSNAAVTVDLFH 797 Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 63/166 (37%), Positives = 86/166 (51%), Gaps = 39/166 (23%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSAST 60 VDL NG G S G+AAGD + NIQ++ GS+ +DTF+A+ N FDG GS+TVSY+ ST Sbjct: 1047 VDL--FNGRG-SNGFAAGDTYANIQNVIGSAYDDTFVADNNHNVFDGRQGSNTVSYANST 1103 Query: 61 VSVTVDLSSG-------------------------------GAGVGEGGXGAGGTSDTVS 89 +VTV+L +G A +GG A G + VS Sbjct: 1104 AAVTVNLVTGTGTGGFADGDTFTNIQNVVGSAQDDLIIASSDANTIDGGLSATGAHNRVS 1163 Query: 90 YEYSNANGAIIANLGTHAGT---GGDANGDTYTNIENLTGSAFLDS 132 Y+N+ + +L GT GG A GD +NI++LTGS F D+ Sbjct: 1164 --YANSGAGVTVDLTYTDGTGTSGGYATGDKLSNIQDLTGSQFDDT 1207 Score = 64.7 bits (156), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 97/282 (34%), Positives = 130/282 (46%), Gaps = 61/282 (21%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGS-DTVSYSAS 59 ++L S GSGT AAGD ++NIQD+TG GNDTF N AN FDGG G+ + VSY+ S Sbjct: 1824 INLVSGQGSGT---LAAGDTYLNIQDVTGGGGNDTFYGNTAANTFDGGGGTHNRVSYATS 1880 Query: 60 TVSVTVDLSSGGAGVGEGGXGAGGTSDTVSYEYSN--------ANGAIIANLGTHA--GT 109 TV+ TVDL G G GG G+ DT Y+N AN +A+ +A G Sbjct: 1881 TVAETVDLF---LGQGSGGAGSLANGDT----YTNIQDATGGSANDTFVASAAANAFDGG 1933 Query: 110 GGDANGDTYTN--------IENLTGSAFLDSTLTGDANANILTAKGTATTNAFSGGGAAS 161 GG N +Y N + + TG+ S GD ANI G + + AA Sbjct: 1934 GGSHNRVSYANSTVDETINLVSNTGTGGAGSLANGDTYANIQDVTGGSGNDTIVASAAA- 1992 Query: 162 GTDVFNVVDGGTNSVAV----------GTGLNTINVSAGSHSSLGAQ------------S 199 N +DGGT S V TG+ T+++SAG+ S AQ S Sbjct: 1993 -----NFLDGGTGSNTVSYAGSTSSNGSTGV-TVDLSAGTGSGNYAQGDTYANIQNVIGS 2046 Query: 200 DMVNQTTGLSNI---STINGAAGTTTLHFADLGSSITLANFA 238 D + TG + + S + G G L D + T A++A Sbjct: 2047 DFADTLTGFATVGVTSVLTGGKGADKLTAIDANRAYTYASYA 2088 Score = 62.8 bits (151), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 51/143 (35%), Positives = 71/143 (49%), Gaps = 36/143 (25%) Query: 12 SGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSASTVSVTVDLSSGG 71 SGGYA + + +IQ+I GSS +DTFIA+ ANNF+GGLG Sbjct: 739 SGGYAQNNSYTHIQNIVGSSYSDTFIASLAANNFNGGLGG-------------------- 778 Query: 72 AGVGEGGXGAGGTSDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLD 131 SDTVSY +SNA A+ +L G+GG A GD +I+N+ GS + D Sbjct: 779 -------------SDTVSYAFSNA--AVTVDLFHGTGSGGYAQGDVLAHIQNVIGSNY-D 822 Query: 132 STLTGDANANILTAKGTATTNAF 154 T A+ N +G + T ++ Sbjct: 823 DTFIASADVNNFDGRGGSNTVSY 845 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 50/130 (38%), Positives = 66/130 (50%), Gaps = 34/130 (26%) Query: 12 SGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGSDTVSYSASTVSVTVDLSSGG 71 SGGYA GD +IQ++ GS+ +DTFIA+ NNFDG GS+TVSY ST VTVDL++ Sbjct: 801 SGGYAQGDVLAHIQNVIGSNYDDTFIASADVNNFDGRGGSNTVSYFYSTGGVTVDLTNTI 860 Query: 72 AGVGEGGXGAGGTSDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYTNIENLTGSAFLD 131 + T Y A GD+++NI+NL GS + D Sbjct: 861 G------------TGTGGYY---------------------AAGDSFSNIQNLIGSVY-D 886 Query: 132 STLTGDANAN 141 T A+AN Sbjct: 887 DTFVASADAN 896 Score = 58.2 bits (139), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 51/127 (40%), Positives = 63/127 (49%), Gaps = 39/127 (30%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLG-SDTVSYSAS 59 VDL + GSG G A GD VNI ++ GS+ +DTF+A+ ANNFDGG G SDTVSY+ Sbjct: 2238 VDLKNATGSG---GDAQGDTLVNIDNVIGSAYSDTFVASTQANNFDGGSGGSDTVSYAND 2294 Query: 60 TVSVTVDLSSGGAGVGEGGXGAGGTSDTVSYEYSNANGAIIANLGTHAGTGGDANGDTYT 119 T VTVDL++ T A +G A GD +T Sbjct: 2295 TGGVTVDLTN-----------------------------------TLAASGAYAVGDKFT 2319 Query: 120 NIENLTG 126 NI NL G Sbjct: 2320 NIANLIG 2326 Score = 58.2 bits (139), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 59/160 (36%), Positives = 77/160 (48%), Gaps = 34/160 (21%) Query: 1 VDLNSTNGSGTSGGYAAGDRFVNIQDITGSSGNDTFIANGVANNFDGGLGS--DTVSYSA 58 VDL + GSG +G A GD + NIQD+TG + +D FIA+ AN FDGG GS + VSY+ Sbjct: 1432 VDLFNNVGSGGAGSLANGDTYANIQDVTGGTADDVFIASAAANAFDGGGGSLHNRVSYAN 1491 Query: 59 STVSVTVDL-----------------------SSGGAG--------VGEGGXGAGGTSDT 87 S+ TV+L +GG+G G GGT + Sbjct: 1492 SSADETVNLVTGQGSGGTGSLANGDTYINIQDVTGGSGNDTFYGNTAANNFDGGGGTHNR 1551 Query: 88 VSYEYSNANGAI-IANLGTHAGTGGDANGDTYTNIENLTG 126 V+Y S I + N G G ANGD YTNI++ TG Sbjct: 1552 VNYSSSTVAETIDLLNGQGSGGAGSLANGDRYTNIQDATG 1591 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.306 0.126 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 719,086 Number of extensions: 28538 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 16 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 130 (54.7 bits)