BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0065.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 519 e-148 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 137 8e-34 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 134 7e-33 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 83 3e-17 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 69 6e-13 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 519 bits (1336), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/287 (86%), Positives = 267/287 (93%), Gaps = 2/287 (0%) Query: 1 LKP-APQNKLDSVKEFDFTNRDFDRVRDLIYKRAGIALAESKQEMVYSRLARRLRATGIA 59 +KP P S KEFDF +RDF+RVR LIY+RAGIALA+SKQEMVYSRLARRLRATGI Sbjct: 1 MKPTVPSKGESSGKEFDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGIT 60 Query: 60 SFVQYLDMLERGGDTDEWEAFTNALTTNLTSFFRESHHFPILADFVKTLKEPATVWCSAA 119 SF QYLDMLER D++EWEAFTNALTTNLTSFFRE+HHFPILADF+KTLKEPAT+WCSAA Sbjct: 61 SFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFLKTLKEPATIWCSAA 120 Query: 120 STGEEPYTIAMTLCEAYGTLTPPAQIIATDIDTNVLATGANGVYPIERIDKLSPERIKKF 179 STGEEPYTIAMT CEAYG+LTPP QI+ATDIDTNVLATG+NGVYP+ERI+K+SPER+KKF Sbjct: 121 STGEEPYTIAMTACEAYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKF 180 Query: 180 FLRGKGNQEGYVRVRPELRKLVTFKQLNLLADGWPLTGQFDVIFCRNVMIYFDKPTQSKI 239 FLRGKG+ EGYVRVRPELRKL+TFKQLNLLADGWPL+GQFDVIFCRNVMIYFDKPTQSKI Sbjct: 181 FLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKI 240 Query: 240 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYELGAATQRKA 286 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYEL ATQR A Sbjct: 241 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYELN-ATQRGA 286 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 137 bits (346), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 87/275 (31%), Positives = 136/275 (49%), Gaps = 16/275 (5%) Query: 21 DFDRVRDLIYKRAGIALAESKQEMVYSRLARRLRATGIASFVQYLDMLERGGDTDEWEAF 80 DF + R+ Y++ GI+ +K+ V RL R+ ATG SF Y +L D E + Sbjct: 10 DFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLL 69 Query: 81 TNALTTNLTSFFRESHHFPILADFV-------KTLKEPATVWCSAASTGEEPYTIAMTLC 133 TN +T N T F RE + F L + + + P +WC S+GEE YT+AM L Sbjct: 70 TNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLAMYLM 129 Query: 134 EAY-GTLTPPAQIIATDIDTNVLATGANGVYPIERIDKLSPERIKKFFLRGKGNQEGYVR 192 E + G +II++DIDT VL G Y + + + + K+FLR +G++ + Sbjct: 130 ERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLR-QGDE---YQ 185 Query: 193 VRPELRKLVTFKQLNLL-ADGWPLTGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHA 251 + LR V F ++NL + FDVIFCRN++IYFD ++ + F + + Sbjct: 186 ICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNG 245 Query: 252 LLFAGHSENFLYVSDALKLKG---KTVYELGAATQ 283 + GHSE+ +S +++ VY+ A Q Sbjct: 246 FICLGHSESMSRISSLYRVRKFPEAIVYQKTAEAQ 280 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 134 bits (338), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 87/270 (32%), Positives = 138/270 (51%), Gaps = 17/270 (6%) Query: 16 DFTNRDFDRVRDLIYKRAGIALAESKQEMVYSRLARRLRATGIASFVQYLDMLERGGDTD 75 + ++ D + L ++ G+ L SK+ ++ SRLA RL G+ QY ++ Sbjct: 12 EVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQ 71 Query: 76 EWEAFTNALTTNLTSFFRESHHFPILADFV------KTLKEPAT-VWCSAASTGEEPYTI 128 E + + +TTN T FFRE HF DFV + ++P +W +A++TGEE YT+ Sbjct: 72 ERQRAIDLITTNETYFFREPGHF----DFVVGQWLPQLAQQPQLRIWSAASATGEEAYTL 127 Query: 129 AMTLCEAYGTLTPPAQ--IIATDIDTNVLATGANGVYPIERIDKLSPERIKKFFLRGKGN 186 AM L P A + A+DI T VL +YP+ R + ++++ L G G Sbjct: 128 AMLLDHH----RPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGE 183 Query: 187 QEGYVRVRPELRKLVTFKQLNLLADGWPLTGQFDVIFCRNVMIYFDKPTQSKILSRFVPL 246 EG+ V+ +LR V F Q NL GQ+D+IF RNV+IYFD T+ ++L + Sbjct: 184 YEGHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIAR 243 Query: 247 MKPHALLFAGHSENFLYVSDALKLKGKTVY 276 ++P L GHSE+ + L ++ ++Y Sbjct: 244 LRPGGYLVVGHSESLHGMDLGLDMQSPSIY 273 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 82.8 bits (203), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 67/193 (34%), Positives = 97/193 (50%), Gaps = 17/193 (8%) Query: 89 TSFFRESHHFPILADFV--KTLKEPATV---WCSAASTGEEPYTIAMTLCEAYGTLTPPA 143 T FFR+ +A K +PA V STGEEPY++AM L +A LTP Sbjct: 69 TWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA--GLTPGQ 126 Query: 144 QII-ATDIDTNVLATGANGVYPIE--RIDKLSPERIKKFFLRGKGNQEGYVRVRPELRKL 200 +I A DI T L A G+Y R +L+ R + F L ++G R+ +R Sbjct: 127 FVIDAYDIRTRSLEIAAAGLYGRNSFRGQELA-FRDRHFDL-----EQGQWRLHERIRSQ 180 Query: 201 VTFKQLNLLADGWPLTGQ-FDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSE 259 V F NLLA + Q +D IFCRNV+IYF++ Q +++ +MKP AL+F G +E Sbjct: 181 VRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAE 240 Query: 260 NFLYVSDALKLKG 272 + + ++ G Sbjct: 241 GGILLGPTMESAG 253 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 55/192 (28%), Positives = 85/192 (44%), Gaps = 11/192 (5%) Query: 84 LTTNLTSFFRESHHFPILADFVKTLKE---PATVWCSAASTGEEPYTIAMTLCEAYGTLT 140 LT ++ FR+ +F L + V L +W + S+GEE Y++A+ L E L Sbjct: 78 LTVQVSEMFRDPSYFLALREKVVPLLRTYPSLKIWVAGCSSGEEVYSLAILLREE--GLL 135 Query: 141 PPAQIIATDIDTNVLATGANGVYPIERIDKLSPERIKKFFLRGKGN----QEGYVRVRPE 196 + I ATDI+ + L GVY +ERI + K + G V Sbjct: 136 ERSIIYATDINQSALKKAEAGVYDLERIAGFTNNHQKSGARTSLSDYYTAAYGAVVFDKS 195 Query: 197 LRKLVTFKQLNLLADGWPLTGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAG 256 LRK V F +L D + + ++ CRNV+IYF++ Q + L F + L G Sbjct: 196 LRKNVVFSDHSLATD--SVFAEMHLVSCRNVLIYFNRQLQERALGLFRESLVRKGFLGLG 253 Query: 257 HSENFLYVSDAL 268 E+ + S A+ Sbjct: 254 SKESLRFSSHAV 265 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 872,045 Number of extensions: 34997 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 5 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)