BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0039.p (248 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 437 e-124 gi|300311353|ref|YP_003775445.1| flagellar basal body rod protei... 90 2e-19 gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 78 6e-16 gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 71 6e-14 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 437 bits (1123), Expect = e-124, Method: Compositional matrix adjust. Identities = 215/260 (82%), Positives = 233/260 (89%), Gaps = 12/260 (4%) Query: 1 MIRSLWIAKTGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQT 60 MIRSLWI+KTGLDAQQTQLDVISNNLAN STNGFKRSRAVFEDL+YQT+RQPGAQSSQQT Sbjct: 1 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT 60 Query: 61 QLPSGLQIGTGVRPVATERIHTQGNTNQTNNDKDVAIQGAGFFQVLMPDGTTQYTRDGSF 120 QLPSGLQIGTGVRPVATERI TQGN NQTNNDKD+AIQG GFFQ+L+PDGTT YTRDG+F Sbjct: 61 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF 120 Query: 121 QSDAQGQLVTSSGYVVQPPITMPANMTKLTIGRDGTVSIMQAGSTATTQIGTLQIATFIN 180 Q+D+QGQLVTSSGY +QPPIT+P N TKLT+GRDG VSIMQAGSTATTQIGT+Q+ATFIN Sbjct: 121 QTDSQGQLVTSSGYTLQPPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFIN 180 Query: 181 PAGLESKGENXKKKKKP------------GAGALWQGYVETSNVNVVEEMVNMIQTQRAY 228 PAGLES+GEN + GAGALWQGYVETSNVNVVEE+VNMIQTQRAY Sbjct: 181 PAGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAY 240 Query: 229 EMNSKAITTSDQMLAKLSQL 248 E+NSKAITTSDQMLAKLSQL Sbjct: 241 EINSKAITTSDQMLAKLSQL 260 >gi|300311353|ref|YP_003775445.1| flagellar basal body rod protein FlgF [Herbaspirillum seropedicae SmR1] Length = 245 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 86/260 (33%), Positives = 115/260 (44%), Gaps = 31/260 (11%) Query: 1 MIRSLWIAKTGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQT 60 M R ++ A +G Q SNNLAN +T GF+ F Sbjct: 1 MDRLVYTAMSGAKHTLEQQATASNNLANATTTGFRAQLNTFR---------------AAP 45 Query: 61 QLPSGLQIGTGVRPVATERIHTQGNTNQTNNDKDVAIQGAGFFQVLMPDGTTQYTRDGSF 120 + GL T V +QG T DVAIQG G+ V DG YTR+GSF Sbjct: 46 VVGDGLPTRTFVVDSTAGNDFSQGPIQDTGRALDVAIQGRGWIAVQTADGGEAYTRNGSF 105 Query: 121 QSDAQGQLVTSSGYVV---QPPITMPANMTKLTIGRDGTVSIMQAGS--TATTQIGTLQI 175 + + G L T SG V PIT+P + T + I DGTVS + A +G +++ Sbjct: 106 KINENGILQTQSGQTVLGDGGPITIPPDAT-VAIAADGTVSAIPTNGIPNAVNVLGRIKL 164 Query: 176 ATFINPAGLESK-GENXKKKKKPGAG------ALWQGYVETSNVNVVEEMVNMIQTQRAY 228 +NP K G++ + GA L G +E SNVNVVE MVNMI R + Sbjct: 165 ---VNPDEKNMKRGDDGLFRTITGAAQPDANVRLASGALEGSNVNVVEAMVNMINLSRQF 221 Query: 229 EMNSKAITTSDQMLAKLSQL 248 EMN K I T++ K + + Sbjct: 222 EMNMKMIQTAESDATKATSI 241 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 78.2 bits (191), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 49/131 (37%), Positives = 69/131 (52%), Gaps = 20/131 (15%) Query: 10 TGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQTQLPSGLQIG 69 +GL+ LDVI NN+AN ST GFK+S+A F D+ ++ + G +G Sbjct: 8 SGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNS-----------PVG 56 Query: 70 TGVRPVATERIHTQGNTNQTNNDKDVAIQGAGFFQV--LMPDGTTQYTRDGSFQSDAQGQ 127 GV A + TQGN + +NN D+AI G GFFQ+ + + + Y R+G FQ D Sbjct: 57 IGVSVPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLD---- 112 Query: 128 LVTSSGYVVQP 138 SGY+V P Sbjct: 113 ---KSGYIVNP 120 Score = 54.7 bits (130), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 37/118 (31%), Positives = 57/118 (48%), Gaps = 13/118 (11%) Query: 143 PANMTKLTIGRDGTVSIMQAGSTATTQIGTLQIATFINPAGLESKGENXKKKK------- 195 P +++ ++ +DG++ I + T +GT+ + F NP GL+ G N + Sbjct: 315 PGSLSSFSVDKDGSI-IGSYSNQQTKNLGTVVLVNFANPNGLQPLGNNLYQASSAAGSPL 373 Query: 196 --KPGAGA---LWQGYVETSNVNVVEEMVNMIQTQRAYEMNSKAITTSDQMLAKLSQL 248 KP G L E SNV++ E+VNMI QR Y+ NS+ I D +L L L Sbjct: 374 VGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 431 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 71.2 bits (173), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 45/125 (36%), Positives = 62/125 (49%), Gaps = 13/125 (10%) Query: 10 TGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQTQLPSGLQIG 69 +GL LDVI NN+AN ST GFK+ + F D ++ + G +G Sbjct: 8 SGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSP-----------VG 56 Query: 70 TGVRPVATERIHTQGNTNQTNNDKDVAIQGAGFFQVL--MPDGTTQYTRDGSFQSDAQGQ 127 GV + TQGN TNN DVAI G GFF++ + D + +Y R+G FQ + G Sbjct: 57 IGVTVANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGY 116 Query: 128 LVTSS 132 L+ S Sbjct: 117 LINPS 121 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 41/119 (34%), Positives = 59/119 (49%), Gaps = 15/119 (12%) Query: 143 PANMTKLTIGRDGTVSIMQAGSTATTQI-GTLQIATFINPAGLESKGENXKKKKKPGA-- 199 P ++ +IG+DG SI+ A S T++ G + + F NP GL+ G N + + Sbjct: 303 PGTLSSFSIGKDG--SIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASEAAGSA 360 Query: 200 ----------GALWQGYVETSNVNVVEEMVNMIQTQRAYEMNSKAITTSDQMLAKLSQL 248 GAL VE SNV++ E+VNMI QR Y+ NS+ I D +L L L Sbjct: 361 LVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.126 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 672,881 Number of extensions: 26263 Number of successful extensions: 72 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 64 Number of HSP's successfully gapped: 6 Length of query: 248 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 165 Effective length of database: 1,226,865 Effective search space: 202432725 Effective search space used: 202432725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)