BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0011.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 pro... 281 6e-77 gi|300310454|ref|YP_003774546.1| flagellar hook-associated prote... 256 1e-69 gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herb... 210 1e-55 >gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 protein [Herbaspirillum seropedicae SmR1] Length = 690 Score = 281 bits (718), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 154/251 (61%), Positives = 192/251 (76%), Gaps = 4/251 (1%) Query: 5 IQSAGYPSSQQFTAGDSVAIKIGT---NPPTFVTLQANATLAGVRDAINASKAGVTASIT 61 + S S Q ++AG+ + +K+G N N L V+ AIN + AGVTASIT Sbjct: 334 VTSPNVASGQTYSAGN-LTLKVGDSSFNIAPVANGSGNIDLTQVKLAINNANAGVTASIT 392 Query: 62 NDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLSGSTMGQLQAAQDAKLSIDGTTVTSASN 121 NDG+ DHLVLTP+GS AT+ VSLTGT D+ LS S MGQL AQDAKLSIDG VTS +N Sbjct: 393 NDGSNDHLVLTPTGSSATAAVSLTGTSDYAGLSMSGMGQLLKAQDAKLSIDGVNVTSPTN 452 Query: 122 TVKDAISGVTLNLTKVTTAADNFSLTISNDTTGVTSTATTFVSAYNALAKSIADLTKQTP 181 V++ ISGVTLNL+KVTT+ADNF+L ISNDT+G+T+ A +FV+AYN LAK++A +T QTP Sbjct: 453 KVENVISGVTLNLSKVTTSADNFTLNISNDTSGITTAANSFVTAYNTLAKAVAGMTAQTP 512 Query: 182 ATTLGQAGTSSPLASESSVQSILTQLRSALLGNVPGGSGISSLTDIGIGFQKDGTLALDA 241 +TTLGQA TS+PLASESSVQ+I++QLR+ L V GG+GI++L+DIGI FQKDGTLALD+ Sbjct: 513 STTLGQANTSAPLASESSVQTIMSQLRNTLFSTVDGGNGINTLSDIGISFQKDGTLALDS 572 Query: 242 TKLSTATTKNF 252 TKLSTA T NF Sbjct: 573 TKLSTAATNNF 583 Score = 112 bits (281), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 67/114 (58%), Positives = 76/114 (66%), Gaps = 7/114 (6%) Query: 1 LAQKIQSAGYPSSQQFTAGDSVAIKIGTNPPTFVTLQANATLAGVRDAINASKAGVTASI 60 LAQKIQSAG S F AGDS+AIKIGTN P FVTLQAN+TLAGVRDAINA K G TASI Sbjct: 106 LAQKIQSAGISSGTTFNAGDSIAIKIGTNSPVFVTLQANSTLAGVRDAINAGKTGATASI 165 Query: 61 TNDGTQDHLVLTPSGSGATSPVSLTG----TGDFVNLSG---STMGQLQAAQDA 107 DG DHLVL + G + + +T +G F + S STM Q QA +DA Sbjct: 166 VTDGAGDHLVLESNTGGTANTIKVTANNSLSGFFYDPSSSTPSTMTQTQAPRDA 219 Score = 71.2 bits (173), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 60/164 (36%), Positives = 88/164 (53%), Gaps = 13/164 (7%) Query: 2 AQKIQSAGYPSSQQFTAGDSVAIKIGTNPPTFVTLQANATLAGVRDAINASKAGVTASIT 61 A+K+ SA F G +AIK G+ T + N TLAGVRDAIN+S AGV A+I Sbjct: 237 AEKLSSASIAPGSTFDNG-LLAIKTGSGSTTIIKPTTN-TLAGVRDAINSSDAGVNATIV 294 Query: 62 NDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLSGSTMGQLQAAQDAKLSIDGTTVTSASN 121 +DGT DHLV+T SG+T+ + +TGT +F + G + + A G T ++ + Sbjct: 295 SDGTNDHLVVTAKNSGSTNTIKITGTENFAVFNSDPSGTVTSPNVAS----GQTYSAGNL 350 Query: 122 TVKDAISGVTLNLTKVTTAADNFSLT-----ISNDTTGVTSTAT 160 T+K + + N+ V + N LT I+N GVT++ T Sbjct: 351 TLK--VGDSSFNIAPVANGSGNIDLTQVKLAINNANAGVTASIT 392 >gi|300310454|ref|YP_003774546.1| flagellar hook-associated protein 2 [Herbaspirillum seropedicae SmR1] Length = 676 Score = 256 bits (655), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 144/252 (57%), Positives = 192/252 (76%), Gaps = 9/252 (3%) Query: 5 IQSAGYPSSQQFTAGDSVAIKIGTNP----PTFVTLQANATLAGVRDAINASKAGVTASI 60 I + G ++Q +++G S+A+++G+ PT + L V AIN + GVTASI Sbjct: 334 ITTTGVATNQTYSSG-SLALQVGSKSFTINPTDLDGSGAIGLNDVMKAINDANTGVTASI 392 Query: 61 TNDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLSGSTMGQLQAAQDAKLSIDGTTVTSAS 120 TNDG++DHLVLTPSG T V LTG GD+ +L+GS+MGQL AQDAKL++DG TVTS S Sbjct: 393 TNDGSKDHLVLTPSG---TDAVKLTGAGDYSDLAGSSMGQLAKAQDAKLTVDGVTVTSTS 449 Query: 121 NTVKDAISGVTLNLTKVTTAADNFSLTISNDTTGVTSTATTFVSAYNALAKSIADLTKQT 180 N V + ISGVTLNL+KVTT+ADNFSL+I+NDT+G+++ A TFV+AYN LAK++A+LTKQT Sbjct: 450 NKVTNVISGVTLNLSKVTTSADNFSLSINNDTSGLSTAANTFVTAYNTLAKAVANLTKQT 509 Query: 181 PATTLGQAGTSSPLASESSVQSILTQLRSALLGNVPGGSGISSLTDIGIGFQKDGTLALD 240 P+TT GQA T SPLASESSV +++ Q+R+ +LG + G +G ++L+ IGI FQKDGTLALD Sbjct: 510 PSTTKGQASTGSPLASESSVLALMGQVRNTMLGAL-GDNGATNLSQIGISFQKDGTLALD 568 Query: 241 ATKLSTATTKNF 252 ATKL+ A K+F Sbjct: 569 ATKLTNAANKDF 580 Score = 125 bits (313), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 67/114 (58%), Positives = 83/114 (72%), Gaps = 7/114 (6%) Query: 1 LAQKIQSAGYPSSQQFTAGDSVAIKIGTNPPTFVTLQANATLAGVRDAINASKAGVTASI 60 LAQK+QSAG+ + Q F AGDSVAIK+GTN PTF+TLQANATLAGVRDAINA+KAGVTASI Sbjct: 106 LAQKLQSAGFTAGQTFNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASI 165 Query: 61 TNDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLS-------GSTMGQLQAAQDA 107 T DG+ DHLVL + G + + +T +L+ ST+ Q+QA +DA Sbjct: 166 TTDGSGDHLVLESNTGGTANTIKITANNSLSSLAYDPSSNVSSTVTQIQAPRDA 219 >gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herbaspirillum seropedicae SmR1] Length = 680 Score = 210 bits (534), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 115/226 (50%), Positives = 157/226 (69%), Gaps = 8/226 (3%) Query: 31 PTFVTLQANATLAGVRDAINASKAGVTASITNDGTQD----HLVLTPSGSGATSPVSLTG 86 P T A LA V AIN +KAGV+A++ +G D LVL+ SGS PVSL G Sbjct: 365 PASATAGAAPGLADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGS---DPVSLRG 421 Query: 87 TGDFVNLSGSTMGQLQAAQDAKLSIDGTTVTSASNTVKDAISGVTLNLTKVTTAADNFSL 146 +GD+ L+ S MGQLQ QDA+LSIDG +T+ SNT+KDAISGVTLNL K T+A D +L Sbjct: 422 SGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLTL 481 Query: 147 TISNDTTGVTSTATTFVSAYNALAKSIADLTKQTPATTLGQAGTSSPLASESSVQSILTQ 206 ISND++GV + +FV+A+N LAK++A++T+Q PA GQAG LA ES V+++ + Sbjct: 482 AISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGLLAGESMVRALTGE 541 Query: 207 LRSALLGNVPGGSGISSLTDIGIGFQKDGTLALDATKLSTATTKNF 252 LR+A+L ++P G+ + SLT IG+GF+KDGTL LD+ +L+ A +NF Sbjct: 542 LRNAVLASMP-GNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNF 586 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 51/113 (45%), Positives = 69/113 (61%), Gaps = 7/113 (6%) Query: 2 AQKIQSAGYPSSQQFTAGDSVAIKIGTNPPTFVTLQANATLAGVRDAINASKAGVTASIT 61 AQ I+SAG+ S F AGDSVAIK+G P F+TL+A+AT+ G+RD INA++AGVTAS+ Sbjct: 109 AQIIKSAGFASGHLFKAGDSVAIKVGEASPHFITLKADATINGLRDTINAARAGVTASVV 168 Query: 62 NDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLS-------GSTMGQLQAAQDA 107 D DHLVL + +G + + + +S M Q QAA+DA Sbjct: 169 RDDAGDHLVLESNTAGTANLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDA 221 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 49/96 (51%), Positives = 61/96 (63%), Gaps = 4/96 (4%) Query: 1 LAQ--KIQSAGYPSSQQFTAGDSVAIKIGTNPPTFVTLQANATLAGVRDAINASKAGVTA 58 LAQ K+ SAG F G +AIK G+ T + +N TLAGVRDAINA+ AGV A Sbjct: 236 LAQSHKLSSAGIAPGSTFDLG-VLAIKAGSGSTTLIKPASN-TLAGVRDAINAAAAGVNA 293 Query: 59 SITNDGTQDHLVLTPSGSGATSPVSLTGTGDFVNLS 94 SI +DG Q+HLVLT SGA + + ++GTGDF S Sbjct: 294 SIVSDGKQEHLVLTAKESGANNALRISGTGDFAVFS 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 820,302 Number of extensions: 30986 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 8 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)