BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0010.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 pro... 288 4e-79 gi|300310454|ref|YP_003774546.1| flagellar hook-associated prote... 281 5e-77 gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herb... 235 2e-63 >gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 protein [Herbaspirillum seropedicae SmR1] Length = 690 Score = 288 bits (736), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 154/226 (68%), Positives = 185/226 (81%), Gaps = 5/226 (2%) Query: 31 GDHLVMESNTAGTANTIKVQANNSLAGLAYDPSSSTPSTVTQIQAPRDATAAASGTYSVS 90 GDHLV+ESNT GTANTIKV ANNSL+G YDPSSSTPST+TQ QAPRDAT AASG Y+V+ Sbjct: 171 GDHLVLESNTGGTANTIKVTANNSLSGFFYDPSSSTPSTMTQTQAPRDATKAASGNYAVA 230 Query: 91 VSQLAQAQKITSTGFAAGYSFDTGILAIKTGTGSTAIIKPATNTLAGVRDAINASDAGVS 150 VSQLAQA+K++S A G +FD G+LAIKTG+GST IIKP TNTLAGVRDAIN+SDAGV+ Sbjct: 231 VSQLAQAEKLSSASIAPGSTFDNGLLAIKTGSGSTTIIKPTTNTLAGVRDAINSSDAGVN 290 Query: 151 ATIVSDGTAAHLVLTAKDSGSANSIRVSGTGSYAALTFDPSGKYSSPGVATGQTYDAGTG 210 ATIVSDGT HLV+TAK+SGS N+I+++GT ++A DPSG +SP VA+GQTY A G Sbjct: 291 ATIVSDGTNDHLVVTAKNSGSTNTIKITGTENFAVFNSDPSGTVTSPNVASGQTYSA--G 348 Query: 211 GLTLKVGNTTTNVA--LSGAGKT-LQDVRDAINTANAGVTASITNE 253 LTLKVG+++ N+A +G+G L V+ AIN ANAGVTASITN+ Sbjct: 349 NLTLKVGDSSFNIAPVANGSGNIDLTQVKLAINNANAGVTASITND 394 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 100/287 (34%), Positives = 140/287 (48%), Gaps = 64/287 (22%) Query: 8 LDPLTSQASSYNSLISAYGNPPXGDHLVMESNTAGTANTIKVQANN-SLAG----LAYDP 62 L PLT+QASSYN+LISAYG+ + A T+ + Q ++ S AG L DP Sbjct: 33 LTPLTTQASSYNTLISAYGSLKSAMSTYQSAIKAMTSASFSAQKSSVSNAGTGSNLTTDP 92 Query: 63 SSSTPSTVTQIQAPRDATAAASGTYSVSVSQLAQAQKITSTGFAAGYSFDTG-ILAIKTG 121 S A +G S + AQKI S G ++G +F+ G +AIK G Sbjct: 93 FS----------------ADINGDDSTKI----LAQKIQSAGISSGTTFNAGDSIAIKIG 132 Query: 122 TGSTAIIK-PATNTLAGVRDAINASDAGVSATIVSDGTAAHLVLTAKDSGSANSIRVSGT 180 T S + A +TLAGVRDAINA G +A+IV+DG HLVL + G+AN+I+V+ Sbjct: 133 TNSPVFVTLQANSTLAGVRDAINAGKTGATASIVTDGAGDHLVLESNTGGTANTIKVTAN 192 Query: 181 GSYAALTFDPSG----------------------------------KYSSPGVATGQTYD 206 S + +DPS K SS +A G T+D Sbjct: 193 NSLSGFFYDPSSSTPSTMTQTQAPRDATKAASGNYAVAVSQLAQAEKLSSASIAPGSTFD 252 Query: 207 AGTGGLTLKVGNTTTNVALSGAGKTLQDVRDAINTANAGVTASITNE 253 G L +K G+ +T + + TL VRDAIN+++AGV A+I ++ Sbjct: 253 NGL--LAIKTGSGSTTI-IKPTTNTLAGVRDAINSSDAGVNATIVSD 296 Score = 58.5 bits (140), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 58/195 (29%), Positives = 88/195 (45%), Gaps = 43/195 (22%) Query: 5 AKVLDPLTSQASSYNSLISAYGNPPXGDHLVMESNTAGTANTIKVQANNSLAGLAYDPSS 64 A V D + S + N+ I + G DHLV+ + +G+ NTIK+ + A DPS Sbjct: 276 AGVRDAINSSDAGVNATIVSDG---TNDHLVVTAKNSGSTNTIKITGTENFAVFNSDPS- 331 Query: 65 STPSTVTQIQAPRDATAAASGTYSVSVSQLAQAQKITSTGFAAGYSFDTGILAIKTGTGS 124 GT +TS A+G ++ G L +K G S Sbjct: 332 --------------------GT-------------VTSPNVASGQTYSAGNLTLKVG-DS 357 Query: 125 TAIIKPATN-----TLAGVRDAINASDAGVSATIVSDGTAAHLVLTAKDSGSANSIRVSG 179 + I P N L V+ AIN ++AGV+A+I +DG+ HLVLT S + ++ ++G Sbjct: 358 SFNIAPVANGSGNIDLTQVKLAINNANAGVTASITNDGSNDHLVLTPTGSSATAAVSLTG 417 Query: 180 TGSYAALTFDPSGKY 194 T YA L+ G+ Sbjct: 418 TSDYAGLSMSGMGQL 432 >gi|300310454|ref|YP_003774546.1| flagellar hook-associated protein 2 [Herbaspirillum seropedicae SmR1] Length = 676 Score = 281 bits (718), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 149/227 (65%), Positives = 184/227 (81%), Gaps = 6/227 (2%) Query: 31 GDHLVMESNTAGTANTIKVQANNSLAGLAYDPSSSTPSTVTQIQAPRDATAAASGTYSVS 90 GDHLV+ESNT GTANTIK+ ANNSL+ LAYDPSS+ STVTQIQAPRDA+ AA+G Y+++ Sbjct: 171 GDHLVLESNTGGTANTIKITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIA 230 Query: 91 VSQLAQAQKITSTGFAAGYSFDTGILAIKTGTGSTAIIKPATNTLAGVRDAINASDAGVS 150 V+QLAQA K++S G A G +FD G+LAIKTG GSTAII+P TN+LAGVRDAINASDAGV+ Sbjct: 231 VNQLAQAVKVSSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVN 290 Query: 151 ATIVSDGTAAHLVLTAKDSGSANSIRVSGTGSYAALTFDPSGKYSSPGVATGQTYDAGTG 210 A+IV+DGT HLVLTAKD+GSAN++RV+GTG++AA FDPSG ++ GVAT QTY +G+ Sbjct: 291 ASIVNDGTNDHLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGS- 349 Query: 211 GLTLKVGNTTTNVA---LSGAGKT-LQDVRDAINTANAGVTASITNE 253 L L+VG+ + + L G+G L DV AIN AN GVTASITN+ Sbjct: 350 -LALQVGSKSFTINPTDLDGSGAIGLNDVMKAINDANTGVTASITND 395 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 102/287 (35%), Positives = 144/287 (50%), Gaps = 64/287 (22%) Query: 8 LDPLTSQASSYNSLISAYG---NPPXGDHLVMESNTAGTANTIKVQANNSLAG--LAYDP 62 L PL ++ SS+N+LISAYG N +++ TA + + K +N+ G L DP Sbjct: 33 LTPLKNKESSFNTLISAYGSLKNAFTSYQSALKTLTAASFSAQKTTLSNAGTGTNLTTDP 92 Query: 63 SSSTPSTVTQIQAPRDATAAASGTYSVSVSQLAQAQKITSTGFAAGYSFDTG-ILAIKTG 121 ++ +T S AQK+ S GF AG +F+ G +AIK G Sbjct: 93 FTADTNT--------------------DESTKVLAQKLQSAGFTAGQTFNAGDSVAIKVG 132 Query: 122 TGS-TAIIKPATNTLAGVRDAINASDAGVSATIVSDGTAAHLVLTAKDSGSANSIRVSGT 180 T T I A TLAGVRDAINA+ AGV+A+I +DG+ HLVL + G+AN+I+++ Sbjct: 133 TNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLESNTGGTANTIKITAN 192 Query: 181 GSYAALTFDPS---------------------GKY-------------SSPGVATGQTYD 206 S ++L +DPS GKY SS G+A G T+D Sbjct: 193 NSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAVKVSSAGIAPGSTFD 252 Query: 207 AGTGGLTLKVGNTTTNVALSGAGKTLQDVRDAINTANAGVTASITNE 253 G L +K GN +T + + +L VRDAIN ++AGV ASI N+ Sbjct: 253 NGV--LAIKTGNGSTAI-IQPKTNSLAGVRDAINASDAGVNASIVND 296 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 48/165 (29%), Positives = 78/165 (47%), Gaps = 42/165 (25%) Query: 27 NPPXGDHLVMESNTAGTANTIKVQANNSLAGLAYDPSSSTPSTVTQIQAPRDATAAASGT 86 N DHLV+ + AG+ANT++V + A +DPS + Sbjct: 295 NDGTNDHLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGT--------------------- 333 Query: 87 YSVSVSQLAQAQKITSTGFAAGYSFDTGILAIKTGTGSTAIIKPATNT-----LAGVRDA 141 IT+TG A ++ +G LA++ G+ S I + L V A Sbjct: 334 -------------ITTTGVATNQTYSSGSLALQVGSKSFTINPTDLDGSGAIGLNDVMKA 380 Query: 142 INASDAGVSATIVSDGTAAHLVLTAKDSGSANSIRVSGTGSYAAL 186 IN ++ GV+A+I +DG+ HLVLT SG+ ++++++G G Y+ L Sbjct: 381 INDANTGVTASITNDGSKDHLVLTP--SGT-DAVKLTGAGDYSDL 422 >gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herbaspirillum seropedicae SmR1] Length = 680 Score = 235 bits (600), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 126/229 (55%), Positives = 166/229 (72%), Gaps = 6/229 (2%) Query: 31 GDHLVMESNTAGTANTIKVQANNSLAGLAYDPSSSTPSTVTQIQAPRDATAAASGTYSVS 90 GDHLV+ESNTAGTAN +KV+ANNSL+ +++D S P +TQ QA RDAT+AA G+Y++S Sbjct: 173 GDHLVLESNTAGTANLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAIS 232 Query: 91 VSQLAQAQKITSTGFAAGYSFDTGILAIKTGTGSTAIIKPATNTLAGVRDAINASDAGVS 150 V QLAQ+ K++S G A G +FD G+LAIK G+GST +IKPA+NTLAGVRDAINA+ AGV+ Sbjct: 233 VEQLAQSHKLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVN 292 Query: 151 ATIVSDGTAAHLVLTAKDSGSANSIRVSGTGSYAALTFDPSGKYSSPGVATGQTYDAGTG 210 A+IVSDG HLVLTAK+SG+ N++R+SGTG +A + DPSG S+ V + + G Sbjct: 293 ASIVSDGKQEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFS--QG 350 Query: 211 GLTLKVGNTTTNV----ALSGAGKTLQDVRDAINTANAGVTASITNEVQ 255 + L VG T ++ A +GA L DV AIN A AGV+A++ E Q Sbjct: 351 AVNLSVGARTISLKPASATAGAAPGLADVARAINEAKAGVSATVQREGQ 399 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 101/285 (35%), Positives = 136/285 (47%), Gaps = 58/285 (20%) Query: 8 LDPLTSQASSYNSLISAYGNPPXGDHLVMESNTAGTANTIKVQANNSLAGLAYDPSSSTP 67 L PL S+A S+N+LISAYG GTA T N+LA L S+ Sbjct: 33 LTPLASKADSFNALISAYGK-------------LGTALTTY---QNALAELTAAKFSAQK 76 Query: 68 STVTQIQAPRDATA---AASGTYSVSVSQLAQAQKITSTGFAAGYSFDTG-ILAIKTGTG 123 + V+ D + A S + S AQAQ I S GFA+G+ F G +AIK G Sbjct: 77 AAVSNTGRSSDGKSGNDAVSADINSDASTKAQAQIIKSAGFASGHLFKAGDSVAIKVGEA 136 Query: 124 STAIIK-PATNTLAGVRDAINASDAGVSATIVSDGTAAHLVLTAKDSGSANSIRVSGTGS 182 S I A T+ G+RD INA+ AGV+A++V D HLVL + +G+AN ++V S Sbjct: 137 SPHFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLESNTAGTANLLKVRANNS 196 Query: 183 YAALTFD----------------------------------PSGKYSSPGVATGQTYDAG 208 +A++FD S K SS G+A G T+D G Sbjct: 197 LSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSHKLSSAGIAPGSTFDLG 256 Query: 209 TGGLTLKVGNTTTNVALSGAGKTLQDVRDAINTANAGVTASITNE 253 L +K G+ +T + + A TL VRDAIN A AGV ASI ++ Sbjct: 257 V--LAIKAGSGSTTL-IKPASNTLAGVRDAINAAAAGVNASIVSD 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.306 0.121 0.321 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 794,936 Number of extensions: 34558 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 8 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 129 (54.3 bits)