BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0101.0012.p
         (302 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Her...   451   e-128
gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri...    60   3e-10

>gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score =  451 bits (1160), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 223/303 (73%), Positives = 245/303 (80%), Gaps = 2/303 (0%)

Query: 1   MREHHASPARDDIRGNRYAAEFFEHEYRLDYEIHTYPKPILCWGHGIVMGGGIGLMAGCS 60
           MREHHASPAR+DIR NRYAAEFFE EYRLDY IH++ KPILCWGHGIVMGGGIGLMAGCS
Sbjct: 82  MREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKPILCWGHGIVMGGGIGLMAGCS 141

Query: 61  HRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGKLGAFLALTGALMNAEDAKFTGLADY 120
           HRVVTE+SRLAMPEITIGLYPDVGGSWFL+R PGK+G FLALTGA +NA+DA F GLADY
Sbjct: 142 HRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIFLALTGANLNAQDALFAGLADY 201

Query: 121 RIAHADKQKVIDALLLQNWGDGDDAAVLGRVL--QQAERDVAADVAFAPSALRENFDLIN 178
            +AHA K  V DALL Q WG G DA +L RVL   QA        AF PS LR +FD I 
Sbjct: 202 GMAHASKAAVFDALLSQPWGQGRDAELLDRVLAGAQAASRREGVAAFVPSNLRSHFDQIE 261

Query: 179 ALCSGQTVPDIVDAILAHKTDNPWLQKAIATLTAGAPGSAALALALQQRVRHMSLAEVFR 238
           ALC   T+P+IVDAILA +TD+ WL KA +TL AGAPGSA L  ALQ++VR +SLAEVFR
Sbjct: 262 ALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGSAWLGYALQKKVRPLSLAEVFR 321

Query: 239 LEFVVTLHCATRPDFVEGIRALIIEKDQKPKWNPARLADATPQWVDGFFADPWPASEHPL 298
           LEFVV+LHCA RPDFVEGIRALIIEKDQKP+W PA L  ATP WV+GFFADPW    HPL
Sbjct: 322 LEFVVSLHCAARPDFVEGIRALIIEKDQKPQWKPASLKQATPAWVEGFFADPWTQGGHPL 381

Query: 299 ADL 301
           ADL
Sbjct: 382 ADL 384


>gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 258

 Score = 59.7 bits (143), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 35/91 (38%), Positives = 49/91 (53%), Gaps = 2/91 (2%)

Query: 38  KPILCWGHGIVMGGGIGLMAGCSHRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGKLG 97
           KP++    G  +GGG  L   C   +  + ++   PEI +G+ P  GG+  L R   K  
Sbjct: 96  KPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAK 155

Query: 98  AF-LALTGALMNAEDAKFTGLADYRIAHADK 127
           A  LALTG +M+AE+A+  GL   RI  ADK
Sbjct: 156 AMDLALTGRMMDAEEAERAGLVS-RIVAADK 185


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.137    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 929,954
Number of extensions: 35666
Number of successful extensions: 121
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 120
Number of HSP's successfully gapped: 2
Length of query: 302
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 217
Effective length of database: 1,217,395
Effective search space: 264174715
Effective search space used: 264174715
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 130 (54.7 bits)