BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0100.0039.p
         (313 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310608|ref|YP_003774700.1| major facilitator superfamily p...   179   3e-46
gi|300314034|ref|YP_003778126.1| hypothetical protein Hsero_4757...    70   2e-13
gi|300310486|ref|YP_003774578.1| major facilitator superfamily p...    60   3e-10

>gi|300310608|ref|YP_003774700.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 407

 Score =  179 bits (454), Expect = 3e-46,   Method: Compositional matrix adjust.
 Identities = 96/211 (45%), Positives = 131/211 (62%), Gaps = 1/211 (0%)

Query: 33  MAFRLREPKLAHDGKHEQSIWDATREAFAYRPFQFLIAGYFVCGFQVVFIGAHLPSYLKD 92
           +A     P  A   + + S+ +   +A A+R +Q L  G+F CGFQ+ FI  HLP YL +
Sbjct: 186 LALARTGPVAADAPRLDYSVREVIAQATAHRGYQLLTLGFFACGFQLAFIATHLPGYL-E 244

Query: 93  KGLMDPNIAVAALALIGLFNIFGSYYAGKLGGFLPKRYLLSTIYLSRTIAISLFLLAPLS 152
              M   +A  ALALIGLFN+ GS+  G LGG    + +L  +YL R+ AI LF +AP S
Sbjct: 245 LCHMPMGLAATALALIGLFNMAGSWACGWLGGRYRPQRVLGWLYLVRSTAIGLFFIAPKS 304

Query: 153 PWSVYIFSAAMGLLWLSTVPLTNGIIAGIFGLKHLSMLSGFVFLSHQVGSFLGVWLGGYL 212
             SV +F+A MGL WL TVPLT G++A +FG  H+  L G  FLSHQVGSFLG WLGG++
Sbjct: 305 SQSVTLFAAVMGLTWLGTVPLTTGLVARLFGTAHMGTLFGVCFLSHQVGSFLGAWLGGWM 364

Query: 213 FTREGSYNTVWMITIGLGLFATVMNLPVNEK 243
               GSY+ +WM T  +G+ A +++ P+ E 
Sbjct: 365 LEVTGSYSLLWMATFVVGIVAALLHFPIEEA 395


>gi|300314034|ref|YP_003778126.1| hypothetical protein Hsero_4757
           [Herbaspirillum seropedicae SmR1]
          Length = 68

 Score = 70.1 bits (170), Expect = 2e-13,   Method: Composition-based stats.
 Identities = 31/51 (60%), Positives = 39/51 (76%)

Query: 254 RIQRPATGEECALSPEVNALADVYAVMIIEHLHSIAVDLIGKESRQLFEAW 304
           R QRPA+GEECALSPEVNALA +YA+MI    H + ++ I  ++RQL EAW
Sbjct: 15  RQQRPASGEECALSPEVNALAGLYAMMIFHRRHEVDLEQIDAQARQLIEAW 65


>gi|300310486|ref|YP_003774578.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 430

 Score = 59.7 bits (143), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 59/240 (24%), Positives = 107/240 (44%), Gaps = 22/240 (9%)

Query: 1   VMMPVEQQLISVTGWQQAFYWLAALVVAVMVPMAFRL--------------REPKLAHDG 46
           V +P+   ++   GW+   + ++A V  + +P+ + L              +  ++  +G
Sbjct: 161 VFLPLMAAMVGSYGWRSVAFLVSA-VALLAIPLVWLLLPESPAAIGQKMLGQTSEIKDEG 219

Query: 47  KHEQS----IWDATREAFAYRPFQFLIAGYFVCGFQVV-FIGAHLPSYLKDKGLMDPNIA 101
              ++      DA R +     F  L A +FVCG     +IG    +   D G+ + + A
Sbjct: 220 LSRRNPLFIALDALRTSVRMPDFWLLFASFFVCGLSTNGYIGTQFIAMCNDYGINEVSSA 279

Query: 102 VAALALIGLFNIFGSYYAGKLGGFLPKRYLLSTIYLSRTIAISLFLLA-PLSPWSVYIFS 160
            + LA +G+ ++ G+  +G L      R LL   Y  R +A+     A  L  + + IF+
Sbjct: 280 -SILAAMGMLDLVGTTLSGWLSDRYNPRVLLFWYYGLRGLALIFLPYAFGLQYYGLTIFA 338

Query: 161 AAMGLLWLSTVPLTNGIIAGIFGLKHLSMLSGFVFLSHQVGSFLGVWLGGYLFTREGSYN 220
              GL W++TVP T  +   +FG     ++ G++   HQ+G+     L GYL    G+Y 
Sbjct: 339 IFYGLDWIATVPPTVRLANDVFGRLAAPIVFGWIVAGHQLGAATATTLAGYLRASLGNYT 398


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.141    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 948,922
Number of extensions: 39349
Number of successful extensions: 187
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 186
Number of HSP's successfully gapped: 3
Length of query: 313
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 228
Effective length of database: 1,217,395
Effective search space: 277566060
Effective search space used: 277566060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 130 (54.7 bits)